| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
|
||
| 25g | |||
| Other Sizes |
| Animal Protocol |
Bone marrow micronucleus (BMM) assay in mice:
ICR (Crl:CD1) mice (6-8 weeks old, 5 males and 5 females per group) were used. The test substance was administered as a single oral dose at 500, 1000, or 2000 mg/kg body weight. Negative control received 0.5% methylcellulose in purified deionized water. Positive control received cyclophosphamide monohydrate at 50 mg/kg body weight. Mice were euthanized 24 hours after treatment. Additional groups were dosed with water or 2000 mg/kg of Aceturic acid and sacrificed 48 hours after treatment. Femoral bone marrow was collected, and smears were stained with May-Gruenwald-Giemsa stain. Polychromatic erythrocytes (PCEs) were examined microscopically for micronuclei (micronucleated PCEs, MPCEs). [1] Acute oral toxicity study in rats: Male and female Crl:CD Sprague-Dawley rats (n=5/sex/group) received a single oral gavage dose of Aceturic acid dissolved in 0.5% methylcellulose at the OECD limit dose of 2000 mg/kg body weight. Control groups received water or glycine at 2000 mg/kg body weight. Animals were observed for 14 days. [1] Repeated dose (28-day) dietary toxicity study in rats: Sprague-Dawley rats (10 per sex per group) were fed diets containing Aceturic acid at targeted doses of 100, 500, or 1000 mg/kg body weight/day for 28 days. A glycine control group received 1000 mg/kg body weight/day. Dietary concentrations were adjusted weekly based on mean male body weights and historical mean feed consumption (approx. 28 g/rat/day). Diets were prepared on study days -2, 6, 13, and 20, stored frozen (≤20°C), and used within 7 days. Female rats received the same diet concentrations as males, resulting in slightly higher actual doses. Clinical observations, body weights, feed consumption, functional observational battery, motor activity, hematology, coagulation, serum chemistry, and anatomic pathology were assessed. [1] |
|---|---|
| References | |
| Additional Infomation |
N-acetylglycine is an N-acylglycine, where the acyl group is specifically defined as an acetyl group. It is a human metabolite. It is an N-acetyl amino acid and an N-acylglycine. It is the conjugate acid of N-acetylglycine. N-acetylglycine has been reported to exist in fruit flies, Candida tropicalis, and other organisms with relevant data.
|
| Molecular Formula |
C4H7NO3
|
|---|---|
| Molecular Weight |
117.1033
|
| Exact Mass |
117.042
|
| CAS # |
543-24-8
|
| Related CAS # |
N-Acetylglycine-d5;1219805-82-9
|
| PubChem CID |
10972
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
405.1±28.0 °C at 760 mmHg
|
| Melting Point |
207-209 °C(lit.)
|
| Flash Point |
198.8±24.0 °C
|
| Vapour Pressure |
0.0±2.0 mmHg at 25°C
|
| Index of Refraction |
1.454
|
| LogP |
-1.41
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
8
|
| Complexity |
110
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
OKJIRPAQVSHGFK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C4H7NO3/c1-3(6)5-2-4(7)8/h2H2,1H3,(H,5,6)(H,7,8)
|
| Chemical Name |
2-acetamidoacetic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~853.97 mM)
H2O : ~50 mg/mL (~426.99 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 16.67 mg/mL (142.36 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.5397 mL | 42.6985 mL | 85.3971 mL | |
| 5 mM | 1.7079 mL | 8.5397 mL | 17.0794 mL | |
| 10 mM | 0.8540 mL | 4.2699 mL | 8.5397 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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