| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
- The target of MPO-IN-28 is myeloperoxidase (MPO). It exhibits reversible inhibition against MPO with an IC50 value of 4.2 nM, and irreversible inhibition against MPO with a Ki value of 0.8 nM [1]
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|---|---|
| ln Vitro |
- Enzyme inhibition activity: MPO-IN-28 potently inhibits MPO-mediated hypochlorous acid (HOCl) production with an IC50 value of 5.1 nM. It also inhibits MPO-catalyzed tyrosine nitration, with an IC50 value of 6.3 nM. The compound shows no significant inhibitory activity against other heme enzymes (e.g., cytochrome P450 3A4, cyclooxygenase-2) at concentrations up to 10 μM, indicating high selectivity for MPO [1]
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| Enzyme Assay |
- MPO reversible inhibition assay: The reaction mixture contains MPO enzyme, substrate (hydrogen peroxide, H2O2) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Different concentrations of MPO-IN-28 are added to the mixture, which is then incubated at 37°C for 5 minutes. The absorbance change at 405 nm is measured continuously for 3 minutes to calculate the enzyme activity and IC50 value [1]
- MPO irreversible inhibition assay: MPO-IN-28 is pre-incubated with MPO enzyme at 37°C for 30 minutes. After pre-incubation, the substrate mixture (H2O2 and ABTS) is added, and the absorbance at 405 nm is measured as described above. The Ki value is calculated based on the remaining enzyme activity after pre-incubation [1] - MPO-mediated HOCl production assay: The assay uses taurine as a HOCl scavenger, and the reaction mixture includes MPO, H2O2, taurine, and MPO-IN-28. After incubation at 37°C for 10 minutes, the amount of taurine chloramine (a product of HOCl and taurine) is detected by measuring absorbance at 252 nm to determine the IC50 of MPO-IN-28 for inhibiting HOCl production [1] |
| References |
| Molecular Formula |
C11H13N5O
|
|---|---|
| Molecular Weight |
231.253821134567
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| Exact Mass |
231.112
|
| CAS # |
37836-90-1
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| PubChem CID |
2835211
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
0.7
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
17
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| Complexity |
292
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C)C1C=CC2=C(C)N=C(/N=C(\N)/N)N=C2C=1
|
| InChi Key |
ZJBMSSBTCGJZEE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H13N5O/c1-6-8-4-3-7(17-2)5-9(8)15-11(14-6)16-10(12)13/h3-5H,1-2H3,(H4,12,13,14,15,16)
|
| Chemical Name |
2-(7-methoxy-4-methylquinazolin-2-yl)guanidine
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| Synonyms |
NPYLR7-agonist-18 NPYLR7 agonist 18 NPYLR7agonist18
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~54.05 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (8.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (8.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (8.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3243 mL | 21.6216 mL | 43.2432 mL | |
| 5 mM | 0.8649 mL | 4.3243 mL | 8.6486 mL | |
| 10 mM | 0.4324 mL | 2.1622 mL | 4.3243 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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