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| 25mg |
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| 50mg |
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| 100mg |
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ML-SA1 is a potent agonist of TRPML1 (transient receptor potential mucolipin 1) with broad spectrum of antiviral activity. It can inhibit Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity.
| ln Vitro |
It is possible that ML-SA1 (25 μM; 0–14 hours; A549 cells) will directly impact DENV2 entrance into cells [1]. Even at concentrations as high as 200 μM, ML-SA1 (0~200 μM; A549 cells) did not exhibit any cytotoxicity in cell lines. With an IC50 of 8.93 μM, ML-SA1 (0~50 μM; A549 cells) strongly suppresses DENV2 at the RNA level[1]. In A549 cells, the ML-SA1 dose model decreased ZIKV RNA and protein levels; its IC50 value for ZIKV RNA was 52.99 μM. In vitro, ML-SA1 appears to be a useful dye for DENV2 and ZIKV and functions as an activator of TRPML. In order to prevent viral infection, ML-SA1 stimulates lysosomal acidification and staining activity. ML-SA1 prevents the spread of viruses.
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| Cell Assay |
Western Blot analysis [1]
Cell Types: A549 cells Tested Concentrations: 25μM Incubation Duration: 0~14 hrs (hours) Experimental Results: May affect DENV2 entry into host cells. Induces autophagy in Huh7 cells or A549 cells [1]. |
| References |
[1]. Xia Z, et al. ML-SA1, a selective TRPML agonist, inhibits DENV2 and ZIKV by promoting lysosomal acidification and protease activity. Antiviral Res. 2020;182:104922.
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| Molecular Formula |
C22H22N2O3
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|---|---|
| Molecular Weight |
362.4217
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| CAS # |
332382-54-4
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| SMILES |
O=C(C([H])([H])N1C(C2=C([H])C([H])=C([H])C([H])=C2C1=O)=O)N1C2=C([H])C([H])=C([H])C([H])=C2C([H])(C([H])([H])[H])C([H])([H])C1(C([H])([H])[H])C([H])([H])[H]
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~20.83 mg/mL (~57.47 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7592 mL | 13.7961 mL | 27.5923 mL | |
| 5 mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | |
| 10 mM | 0.2759 mL | 1.3796 mL | 2.7592 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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