| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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Purity: ≥98%
Mildronate (Quaterin; Meldonium; Kvaterin; Mildronate), a structural analogue of gammabutyrobetaine and performance-enhancing drug developed by Latvia Institute of Organic Synthesis, is a potent inhibitor of biosynthesis of L-carnitine by gamma-butyrobetaine (GBB) hydroxylase, also a competitive inhibitor of renal carnitine reabsorption.
| ln Vitro |
By targeting PFKP to regulate glycolysis and consequently promote Nrf2's translocation from the cytoplasm to the nucleus, medonium (20–40 μM; 24 hours) reduces lung injury and mitigates oxidative stress and mitochondrial damage under hypoxic settings [3].
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| ln Vivo |
In mice, meldonium (50, 100, or 200 mg/kg; once daily for three days) moderately lessens lung damage brought on by hypoxia [3].
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| Cell Assay |
RT-PCR[3]
Cell Types: Rat alveolar type II epithelial RLE-6TN cells in hypoxia incubator Tested Concentrations: 20, or 40 μM Incubation Duration: 24 h Experimental Results: Dramatically diminished the mRNA expression of PFKP, PDK1, and PKM2 compared with the hypoxia group. Western Blot Analysis[3] Cell Types: Rat alveolar type II epithelial RLE-6TN cells in hypoxia incubator Tested Concentrations: 20, or 40 μM Incubation Duration: 24 h Experimental Results: Dramatically decreased the protein expression of PFKP, PKM2, and LDHA . |
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| Animal Protocol |
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| References |
[1]. Dambrova M, et al. Pharmacological effects of meldonium: Biochemical mechanisms and biomarkers of cardiometabolic activity. Pharmacol Res. 2016 Nov;113(Pt B):771-780.
[2]. Jolanta Pupure, et al. Neuroprotective properties of mildronate, a mitochondria-targeted small molecule. Neurosci Lett. 2010 Feb 12;470(2):100-5. [3]. Daohui Wang, et al. Meldonium Ameliorates Hypoxia-Induced Lung Injury and Oxidative Stress by Regulating Platelet-Type Phosphofructokinase-Mediated Glycolysis. Front Pharmacol. 2022 Apr 5:13:863451. |
| Molecular Formula |
C6H14N2O2
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| Molecular Weight |
147.19
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| CAS # |
76144-81-5
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| Related CAS # |
Meldonium dihydrate;86426-17-7
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| SMILES |
[O-]C(C([H])([H])C([H])([H])N([H])[N+](C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H])=O
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| Synonyms |
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (684.04 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7939 mL | 33.9697 mL | 67.9394 mL | |
| 5 mM | 1.3588 mL | 6.7939 mL | 13.5879 mL | |
| 10 mM | 0.6794 mL | 3.3970 mL | 6.7939 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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