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Mefenamic Acid (CI 473; CN-35355)

Alias: CI-473; CN-35355;CI473; CN35355;CI 473; CN 35355;Mefenamic Acid; Ponstel; Parkemed; Coslan
Cat No.:V1073 Purity: ≥98%
Mefenamic Acid (CI-473; CN-35355; CI 473; CN 35355;Mefenamic Acid; Ponstel; Parkemed; Coslan), a nonsteroidal anti-inflammatory drug (NSAID), is potent, nonselective,competitive inhibitor of the enzymes COX-1 and COX-2 with anti-inflammatory activity.
Mefenamic Acid (CI 473; CN-35355)
Mefenamic Acid (CI 473; CN-35355) Chemical Structure CAS No.: 61-68-7
Product category: COX
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
2g
5g
10g
25g
50g
100g
Other Sizes

Other Forms of Mefenamic Acid (CI 473; CN-35355):

  • Mefenamic acid-d4 (Mefenamic acid d4)
  • Mefenamic Acid-d3 (Mefenamic Acid d3)
  • Mefenamic acid-13C6 (Mefenamic acid 13C6)
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Mefenamic Acid (CI-473; CN-35355; CI 473; CN 35355; Mefenamic Acid; Ponstel; Parkemed; Coslan), a nonsteroidal anti-inflammatory drug (NSAID), is potent, nonselective, competitive inhibitor of the enzymes COX-1 and COX-2 with anti-inflammatory activity. Mefenamic acid displays a wide range of biological acitivity such as anti-inflammatory, analgesic, and antipyretic properties. It also demonstrated potent anti-proliferative activity in vitro against colorectal cancer cells.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Mefenamic acid is a non-steroidal anti-inflammatory drug that inhibits hCOX-1, having IC50 values of 40 nM and 3 μM for hCOX-1 and hCOX-2, respectively [1]. Mefenamic acid (0-100 μM) is cytotoxic to KB, Saos-2, and 1321N cells; however, U-87MG cells are resistant [2].
ln Vivo

Animal Protocol


ADME/Pharmacokinetics
Absorption, Distribution and Excretion
Mefenamic acid is rapidly absorbed after oral administration.
The fecal route of elimination accounts for up to 20% of the dose, mainly in the form of unconjugated 3-carboxymefenamic acid.3 The elimination half-life of mefenamic acid is approximately two hours. Mefenamic acid, its metabolites and conjugates are primarily excreted by the kidneys. Both renal and hepatic excretion are significant pathways of elimination.
1.06 L/kg [Normal Healthy Adults (18-45 yr)]
Oral cl=21.23 L/hr [Healthy adults (18-45 yrs)]
CROSSES PLACENTAL BARRIER IN MONKEYS...EXCRETED IN URINE, BILE &/OR FECES...
ABSORBED SLOWLY FROM GI TRACT...HIGH PERCENTAGE...BOUND TO PLASMA PROTEINS. PEAK PLASMA LEVELS APPEAR IN 2 TO 4 HR...ANALGESIA MAY PERSIST FOR UP TO 6 HR...ABOUT 50%...IS EXCRETED IN URINE WITHIN 24 HR /HUMAN, ORAL/
Metabolism / Metabolites
Mefenamic acid undergoes metabolism by CYP2C9 to 3-hydroxymethyl mefenamic acid, and further oxidation to a 3-carboxymefenamic acid may occur. The activity of these metabolites has not been studied. Mefenamic acid is also glucuronidated directly.
MEFENAMIC ACID...IS TRANSFORMED TO HYDROXYMETHYL DERIVATIVE & TO ACID BY MAJOR, IF NOT ONLY, METABOLIC PATHWAY IN DOG, MONKEY, & HUMAN.
Mefenamic acid has known human metabolites that include 3-hydroxymethyl mefenamic aci.
Biological Half-Life
2 hours
...ABOUT 50%...IS EXCRETED IN URINE WITHIN 24 HR /HUMAN, ORAL/
Toxicity/Toxicokinetics
Effects During Pregnancy and Lactation
◉ Summary of Use during Lactation
Because there is little published experience with mefenamic acid during breastfeeding and it is potentially toxic, other agents may be preferred, especially while nursing a newborn or preterm infant.
◉ Effects in Breastfed Infants
Relevant published information was not found as of the revision date.
◉ Effects on Lactation and Breastmilk
Relevant published information was not found as of the revision date.
Protein Binding
90%
Interactions
...WARFARIN CAN BE DISPLACED FROM PLASMA PROTEIN BY MEFENAMIC ACID...INCR IN FREE WARFARIN CAN RANGE FROM 66-400% WITH CORRESPONDING INCR IN ANTICOAGULANT ACTION. BECAUSE OF DOSAGES INVOLVED, INTERACTIONS ARE MORE LIKELY TO BE IMPORTANT IN PT TREATED WITH WARFARIN THAN IN WORKERS WHO USE IT AS PESTICIDE...
EFFECTS OF ORAL ANTICOAGULANT AGENTS ARE ENHANCED.
References

[1]. Expression and selective inhibition of the constitutive and inducible forms of human cyclo-oxygenase. Biochem J. 1995 Jan 15;305 (Pt 2):479-84.

[2]. In Vitro Cytotoxic Effects of Celecoxib, Mefenamic Acid, Aspirin and Indometacin on Several Cells Lines. J Dent (Shiraz). 2016 Sep;17(3):219-25.

Additional Infomation
Mefenamic acid is an aminobenzoic acid that is anthranilic acid in which one of the hydrogens attached to the nitrogen is replaced by a 2,3-dimethylphenyl group. Although classed as a non-steroidal anti-inflammatory drug, its anti-inflammatory properties are considered to be minor. It is used to relieve mild to moderate pain, including headaches, dental pain, osteoarthritis and rheumatoid arthritis. It has a role as an analgesic, an antirheumatic drug, a non-steroidal anti-inflammatory drug, an antipyretic, an EC 1.14.99.1 (prostaglandin-endoperoxide synthase) inhibitor, an environmental contaminant and a xenobiotic. It is an aminobenzoic acid and a secondary amino compound.
A non-steroidal anti-inflammatory agent with analgesic, anti-inflammatory, and antipyretic properties. It is an inhibitor of cyclooxygenase.
Mefenamic acid is a Nonsteroidal Anti-inflammatory Drug. The mechanism of action of mefenamic acid is as a Cyclooxygenase Inhibitor.
Mefenamic Acid is an anthranilic acid and non-steroidal anti-inflammatory drug (NSAID) with anti-inflammatory, antipyretic and analgesic activities. Mefenamic acid inhibits the activity of the enzymes cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes. The resulting decrease in prostaglandin synthesis, by prostaglandin synthase, is responsible for the therapeutic effects of mefenamic acid. Mefenamic acid also causes a decrease in the formation of thromboxane A2 synthesis, by thromboxane synthase, thereby inhibiting platelet aggregation.
A non-steroidal anti-inflammatory agent with analgesic, anti-inflammatory, and antipyretic properties. It is an inhibitor of cyclooxygenase.
Drug Indication
For the treatment of rheumatoid arthritis, osteoarthritis, dysmenorrhea, and mild to moderate pain, inflammation, and fever.
FDA Label
Mechanism of Action
Mefenamic acid binds the prostaglandin synthetase receptors COX-1 and COX-2, inhibiting the action of prostaglandin synthetase. As these receptors have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity, the symptoms of pain are temporarily reduced.
Therapeutic Uses
Anti-Inflammatory Agents, Non-Steroidal; Cyclooxygenase Inhibitors
MEFENAMIC ACID (PONSTEL) ... PROVIDES ANALGESIA IN MAN SIMILAR TO THAT PRODUCED BY ASPIRIN.
...INDICATED FOR RELIEF OF PAIN RESULTING FROM DENTAL EXTRACTIONS.
DRUG PREFERABLY SHOULD BE TAKEN WITH FOOD.
MEFENAMIC ACID SHOWED FAVORABLE RESULTS RELATIVE TO SOME ANALGESICS.
Drug Warnings
SINCE IT IS NOT SUPERIOR TO ESTABLISHED ANALGESICS & CAN CAUSE SERIOUS TOXICITY, USE OF MEFENAMIC ACID IS NOT RECOMMENDED. IF IT IS USED, ADMIN SHOULD NOT BE EXTENDED BEYOND 7 DAYS. IF DIARRHEA OCCURS, DRUG MUST BE DISCONTINUED & NOT USED AGAIN. IT SHOULD NOT BE USED IN CHILDREN OR IN WOMEN OF CHILDBEARING AGE.
IT IS CONTRAINDICATED IN PT WITH ULCERATION OF UPPER OR LOWER INTESTINAL TRACT...& PT KNOWN TO BE HYPERSENSITIVE TO DRUG. ...CONSIDER ITS USE ONLY IN CASES WHICH EITHER CAN NOT TOLERATE OR DO NOT RESPOND TO LESS TOXIC AGENTS.
MEFENAMIC ACID IS CONTRAINDICATED IN PT...WITH IMPAIRED RENAL FUNCTION, & SHOULD BE USED WITH CAUTION IN ASTHMATICS BECAUSE IT MAY EXACERBATE CONDITION.
Maternal Medication usually Compatible with Breast-Feeding: Mefenamic acid: Reported Sign or Symptom in Infant or Effect on Lactation: None. /from Table 6/
Pharmacodynamics
Mefenamic acid, an anthranilic acid derivative, is a member of the fenamate group of nonsteroidal anti-inflammatory drugs (NSAIDs). It exhibits anti-inflammatory, analgesic, and antipyretic activities. Similar to other NSAIDs, mefenamic acid inhibits prostaglandin synthetase.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C15H15NO2
Molecular Weight
241.29
Exact Mass
241.11
CAS #
61-68-7
Related CAS #
Mefenamic acid-d4;1216745-79-7;Mefenamic Acid-d3;1189707-81-0;Mefenamic acid-13C6;1325559-19-0
PubChem CID
4044
Appearance
White to off-white solid powder
Density
1.2±0.1 g/cm3
Boiling Point
398.8±30.0 °C at 760 mmHg
Melting Point
230 °C
Flash Point
195.0±24.6 °C
Vapour Pressure
0.0±1.0 mmHg at 25°C
Index of Refraction
1.639
LogP
5.33
Hydrogen Bond Donor Count
2
Hydrogen Bond Acceptor Count
3
Rotatable Bond Count
3
Heavy Atom Count
18
Complexity
292
Defined Atom Stereocenter Count
0
InChi Key
HYYBABOKPJLUIN-UHFFFAOYSA-N
InChi Code
InChI=1S/C15H15NO2/c1-10-6-5-9-13(11(10)2)16-14-8-4-3-7-12(14)15(17)18/ h3-9,16H,1-2H3,(H,17,18)
Chemical Name
2-(2,3-dimethylanilino)benzoic acid
Synonyms
CI-473; CN-35355;CI473; CN35355;CI 473; CN 35355;Mefenamic Acid; Ponstel; Parkemed; Coslan
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO:48 mg/mL (198.9 mM)
Water:<1 mg/mL
Ethanol:<1 mg/mL
Solubility (In Vivo)
Solubility in Formulation 1: 2.5 mg/mL (10.36 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.

Solubility in Formulation 2: ≥ 2.5 mg/mL (10.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 4.1444 mL 20.7220 mL 41.4439 mL
5 mM 0.8289 mL 4.1444 mL 8.2888 mL
10 mM 0.4144 mL 2.0722 mL 4.1444 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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g/mol

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Clinical Trial Information
NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01060696 Unknown † Drug: Mefenamic acid or
Hyoscine or placebo
Infertility Mahidol University January 2009 Not Applicable
NCT05064449 CompletedHas Results Drug: Soticlestat
Drug: Itraconazole
Drug: Mefenamic acid
Healthy Volunteers Takeda October 14, 2021 Phase 1
NCT03070678 Completed Drug: Sotagliflozin (SAR439954)
Drug: Mefenamic acid
Diabetes Mellitus Sanofi March 14, 2017 Phase 1
NCT06280014 Completed Procedure: Surgery of impacted
mandibular wisdom teeth
Tooth, Impacted
Tooth Diseases
Yuzuncu Yıl University January 20, 2022 Not Applicable
NCT06369012 Recruiting Drug: Mefenamic acid 500 mg Abnormal Uterine Bleeding Egymedicalpedia March 1, 2024 Not Applicable
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