| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
With IC50 values of 100 and >200 µM, respectively, marein (0-1000 µM; 24 h) suppresses TNFα-induced NF-κB activation as well as HDAC activity [1]. In HepG2 cells, marein (1.25–40 μM; 24 hours) increases the absorption of glucose [2]. Marein (5–10 μM; 24 hours) elevates the amount of liver glycogen, decreases the expression of G6Pase and PEPCK in HepG2 cells, and facilitates the transfer of GLUT1 from intercellular vesicles to the plasma membrane [2]. Marein (5–10 μM; 24 hours) increases the absorption of 2-NBDG and is inhibited by STO-609 and Compound C, an AMPK inhibitor [2]. In PC12 cells, marein (0–40 μM; 24 hours) alters the cytotoxicity of MG [3].
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| Cell Assay |
Western Blot Analysis [2]
Cell Types: HepG2 cell line Tested Concentrations: 5 and 10 μM Incubation Duration: 24 hrs (hours) Experimental Results: The translocation of GLUT1 to the plasma membrane was increased and the phosphorylation levels of AS160 and GSK-3β were diminished in HepG2 cells. Western Blot Analysis[2] Cell Types: HepG2 Cell Line Tested Concentrations: 10 μM Incubation Duration: 0-10 hrs (hours) Experimental Results: Time- and dose-dependent induction of AMPK and Akt phosphorylation in HepG2 cells. Cell viability assay [3] Cell Types: PC12 Cell Line Tested Concentrations: 0-40 μM Incubation Duration: 24 hrs (hours) Experimental Results: Prevents MG-induced loss of PC12 cell viability. Apoptosis analysis[3] Cell Types: PC12 Cell Line Tested Concentrations: 5-10 μM Incubation Duration: 30 minutes Experimental Results: Protection of PC12 cells from MG-induced apoptosis. Western Blot Analysis[3] Cell Types: PC12 Cell Line Tested Concentrations: 5-10 μM Incubation Duration: 30 minutes Experimental Results: Increased Phospho-AMPKα (Thr172) in PC12 cells. |
| References |
[1]. B Orlikova, et al. Natural chalcones as dual inhibitors of HDACs and NF-κB. Oncol Rep. 2012 Sep;28(3):797-805.
[2]. Baoping Jiang, et al. Protective effects of marein on high glucose-induced glucose metabolic disorder in HepG2 cells. Phytomedicine. 2016 Aug 15;23(9):891-900. [3]. Baoping Jiang, et al. Marein protects against methylglyoxal-induced apoptosis by activating the AMPK pathway in PC12 cells. Free Radic Res. 2016;50(11):1173-1187. |
| Molecular Formula |
C21H22O11
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|---|---|
| Molecular Weight |
450.39278
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| CAS # |
535-96-6
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| SMILES |
OC[C@H]1O[C@@H](OC2C=CC(C(/C=C/C3C=CC(O)=C(O)C=3)=O)=C(O)C=2O)[C@H](O)[C@@H](O)[C@@H]1O
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~55.51 mM)
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2203 mL | 11.1015 mL | 22.2030 mL | |
| 5 mM | 0.4441 mL | 2.2203 mL | 4.4406 mL | |
| 10 mM | 0.2220 mL | 1.1101 mL | 2.2203 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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