| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Maltose targets carbohydrate-metabolizing enzymes including α-glucosidase and maltase-glucoamylase, which hydrolyze maltose to glucose for absorption. As a substrate for these enzymes, it is involved in carbohydrate digestion and energy metabolism.
|
|---|---|
| ln Vitro |
Maltose is hydrolyzed by maltase and α-glucosidase enzymes in the small intestine to produce two glucose molecules, which are rapidly absorbed and utilized for energy. In vitro, it serves as a substrate for studying enzyme activity and carbohydrate metabolism.
|
| ln Vivo |
In vivo, maltose is readily digested to glucose by intestinal brush border enzymes and is absorbed, contributing to postprandial blood glucose elevation. It is commonly used in oral rehydration solutions and as an energy source in parenteral and enteral nutrition.
|
| Enzyme Assay |
In vitro enzyme assays for maltose typically involve incubating the disaccharide with α-glucosidase or maltase enzymes and measuring glucose production using glucose oxidase-peroxidase (GOD-POD) or other glucose detection methods. Enzyme activity is determined by the rate of glucose release.
|
| Cell Assay |
In vitro cell-based assays for maltose use intestinal epithelial cell lines (e.g., Caco-2) cultured on transwell inserts to study maltose transport and hydrolysis. Cells are treated with maltose, and glucose transport across the monolayer is measured. Brush border enzyme activity can be assessed in cell lysates.
|
| Animal Protocol |
In vivo animal studies for maltose involve oral administration to rodents followed by measurement of blood glucose levels at various time points to assess glycemic response. Intestinal maltase activity can be measured in tissue homogenates. Standard protocols for carbohydrate tolerance testing are employed.
|
| ADME/Pharmacokinetics |
Maltose monohydrate has a molecular weight of 360.31 g/mol and formula C₁₂H₂₂O₁₁·H₂O. It is highly water-soluble and is rapidly hydrolyzed and absorbed in the small intestine. It has a glycemic index similar to glucose and is used as a readily available energy source.
|
| Toxicity/Toxicokinetics |
Maltose is generally recognized as safe (GRAS) as a food ingredient. It is non-toxic at normal dietary levels. High doses may cause gastrointestinal effects such as osmotic diarrhea in individuals with disaccharidase deficiencies.
|
| References | |
| Additional Infomation |
Maltose monohydrate is widely used as a food additive, sweetener, nutrient, and pharmaceutical excipient. It is an ingredient in oral rehydration solutions, infant formulas, and parenteral nutrition products. It is also used as a substrate in biochemical research for studying carbohydrate metabolism and enzyme activity.
|
| Molecular Formula |
C12H24O12
|
|---|---|
| Molecular Weight |
360.3118
|
| Exact Mass |
360.126
|
| CAS # |
6363-53-7
|
| Related CAS # |
Maltose;69-79-4;Maltose monohydrate-d14
|
| PubChem CID |
23615261
|
| Appearance |
White to off-white solid powder
|
| Density |
1.768g/cm3
|
| Boiling Point |
667.931ºC at 760 mmHg
|
| Melting Point |
119-121 °C (dec.)(lit.)
|
| Flash Point |
357.752ºC
|
| Vapour Pressure |
0mmHg at 25°C
|
| Index of Refraction |
1.652
|
| Hydrogen Bond Donor Count |
9
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
24
|
| Complexity |
367
|
| Defined Atom Stereocenter Count |
9
|
| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@H]([C@@H](CO)O)[C@@H]([C@H](C=O)O)O)O)O)O)O.O
|
| InChi Key |
HBDJFVFTHLOSDW-DNDLZOGFSA-N
|
| InChi Code |
InChI=1S/C12H22O11.H2O/c13-1-4(16)7(18)11(5(17)2-14)23-12-10(21)9(20)8(19)6(3-15)22-12;/h1,4-12,14-21H,2-3H2;1H2/t4-,5+,6+,7+,8+,9-,10+,11+,12+;/m0./s1
|
| Chemical Name |
(2R,3R,4R,5R)-2,3,5,6-tetrahydroxy-4-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexanal;hydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~100 mg/mL (~277.54 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (277.54 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7754 mL | 13.8769 mL | 27.7539 mL | |
| 5 mM | 0.5551 mL | 2.7754 mL | 5.5508 mL | |
| 10 mM | 0.2775 mL | 1.3877 mL | 2.7754 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
