| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| ln Vitro |
magnolioside was evaluated for neuroprotective activity against glutamate-induced toxicity in primary cultured rat cortical cells. At a concentration of 0.1 μM, it exhibited a cell viability of 34.7 ± 3.0% (p < 0.05 vs. glutamate-injured group). At 1 μM, cell viability was 12.2 ± 5.5% (not significant), and at 10 μM, cell viability was 10.2 ± 3.0% (not significant). These results indicate that magnolioside shows weak or negligible neuroprotective activity at higher concentrations, with only marginal activity at 0.1 μM. [1]
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| Cell Assay |
Primary cultures of mixed cortical cells containing both neurons and glia were prepared from 17–19-day-old fetal rats. Cultures were allowed to mature for at least two weeks before use. magnolioside was dissolved in DMSO (final concentration in culture: 0.1%). Cortical cell cultures were washed with DMEM and then incubated with the test compound for 1 hour. Subsequently, the cultures were exposed to 100 μM glutamate and maintained for 24 hours. After incubation, neuronal damage was assessed by measuring the efflux of lactate dehydrogenase (LDH), which reflects the integrity of the cellular membrane. Cell viability was calculated as 100 × (LDH released from glutamate-treated minus LDH released from glutamate + test compound-treated) / (LDH released from glutamate-treated minus LDH released from control). The LDH released from control and glutamate-treated cultures were 12.0 ± 1.3 and 49.5 ± 4.5 units/mL, respectively. [1]
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| References | |
| Additional Infomation |
Magnolioside is a coumarin compound, belonging to the glycoside class. It has been reported to exist in Magnolia denudata, Helichrysum pilosa, and other organisms with relevant data.
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| Molecular Formula |
C16H18O9
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|---|---|
| Molecular Weight |
354.3087
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| Exact Mass |
354.095
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| CAS # |
20186-29-2
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| PubChem CID |
3084335
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| Appearance |
White to off-white solid
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| LogP |
-1.1
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
25
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| Complexity |
510
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| Defined Atom Stereocenter Count |
5
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| SMILES |
O1[C@]([H])([C@@]([H])([C@]([H])([C@@]([H])([C@@]1([H])C([H])([H])O[H])O[H])O[H])O[H])OC1C([H])=C2C([H])=C([H])C(=O)OC2=C([H])C=1OC([H])([H])[H]
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| InChi Key |
WBAVLTNIRYDCPM-YMILTQATSA-N
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| InChi Code |
InChI=1S/C16H18O9/c1-22-9-5-8-7(2-3-12(18)23-8)4-10(9)24-16-15(21)14(20)13(19)11(6-17)25-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1
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| Chemical Name |
7-methoxy-6-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~141.12 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.06 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.06 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (7.06 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8224 mL | 14.1119 mL | 28.2239 mL | |
| 5 mM | 0.5645 mL | 2.8224 mL | 5.6448 mL | |
| 10 mM | 0.2822 mL | 1.4112 mL | 2.8224 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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