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Description: Losartan Potassium (formerly DuP-753; MK-954; DuP753; MK954; Cozaar; Lorzaar; Losaprex), the potassium salt of losartan, is an oral, selective, and non-peptide angiotensin II receptor antagonist approved as an antihypertensive drug. It is an AT II antagonist that competes with angiotensin II for binding to AT1 receptors with IC50 of 20 nM.
References: Circulation. 2001 Feb 13;103(6):904-12; Circulation. 2000 Apr 4;101(13):1586-93.
Related CAS #: 124750-99-8 (Ka+) 114798-26-4 (free)
Product Catalog 2023
Guide to Product Handling
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
In vitro activity: Losartan competes with the binding of angiotensin II to AT1 receptors. The concentration that inhibits 50% of the binding of angiotensin II (IC50) is 20 nM. Losartan (40 μM) affects ISC but prevents the effect of ANGII on ISC. Losartan significantly reduces Ang II-mediated cell proliferation in endometrial cancer cells. The combination of losartan and anti-miR-155 has a significantly greater antiproliferative effect compared to each drug alone.
Cell Assay: An MTT assay is used to measure cell proliferation and viability. For the assay, 5000 cells in 200 μL media per well are seeded in a 96 well plate. After overnight incubation to allow for cell attachment, the medium is removed by suction. MTT at 1 mg/mL concentration in serum-free medium is added and then incubated for 4 h at 37°C. After removal of MTT solution, 100 μL of DMSO is added to dissolve formazan crystals. Absorbance at 570 nm and at 600 nm as a reference is then measured using a microplate reader. The difference in absorbance is thus relative to the extent of cell survival.
Circulation. 2001 Feb 13;103(6):904-12; Circulation. 2000 Apr 4;101(13):1586-93.
Lot#: V177301,Purity ≥98%
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