| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
Linarin targets multiple pathways. It is a selective inhibitor of acetylcholinesterase (AChE). By inhibiting AChE, it increases the concentration of acetylcholine in the synaptic cleft, which can improve cholinergic neurotransmission. It also exhibits significant antioxidant activity, playing a key role in neutralizing free radicals and modulating oxidative stress pathways. Its anti-inflammatory effects are likely mediated through the modulation of inflammatory cytokines.
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| ln Vitro |
In vitro, linarin has been shown to be a selective inhibitor of acetylcholinesterase (AChE). It also demonstrates significant antioxidant activity. These in vitro activities confirm its mechanism as an AChE inhibitor and an antioxidant. Its analgesic, antipyretic, and anti-inflammatory effects have also been demonstrated in various in vitro models.
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| ln Vivo |
In vivo, linarin has demonstrated analgesic, antipyretic, anti-inflammatory, and neuroprotective effects. Its ability to inhibit AChE suggests potential for in vivo efficacy in models of Alzheimer's disease and other cognitive disorders. Its antioxidant and cardioprotective effects also suggest potential therapeutic applications in cardiovascular diseases.
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| Enzyme Assay |
Non-cell-based assays for linarin would typically involve measuring its ability to inhibit acetylcholinesterase (AChE) activity. The enzyme is incubated with a substrate, such as acetylthiocholine, and linarin at various concentrations. The enzymatic activity is measured by the production of a yellow color (thiocholine reacting with DTNB). An IC50 value is determined.
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| Cell Assay |
Cellular assays for linarin are performed using neuronal cell lines to study its neuroprotective effects. The cells are treated with linarin and then exposed to oxidative stress or other insults. Cell viability and markers of oxidative stress are measured. Acetylcholinesterase activity can also be measured in cell lysates.
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| Animal Protocol |
In vivo animal models for linarin would likely involve the use of rodent models of Alzheimer's disease, such as the scopolamine-induced amnesia model. The compound would be administered, and its effects on cognitive function would be assessed using behavioral tests like the Morris water maze. In models of inflammation, its anti-inflammatory effects would be measured.
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| ADME/Pharmacokinetics |
Linarin has a molecular weight of 592.54 g/mol and a molecular formula of C28H32O14. It is a flavone glycoside that is practically insoluble in water and most organic solvents, but soluble in pyridine and concentrated acids and alkalies. Detailed PK parameters such as half-life and bioavailability are not extensively detailed in the provided search results.
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| Toxicity/Toxicokinetics |
Detailed toxicological data for linarin are not provided in the search results. As a natural product, it is generally considered to have a good safety profile, but thorough toxicological studies would be required for therapeutic development. The compound is intended for research use only and is not for human consumption.
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| References | |
| Additional Infomation |
Linarin acid has been reported to be found in Silene firma, Pinus sylvestris, and other organisms with available data.
Linarin is a flavone glycoside found in various plants. It is a selective acetylcholinesterase (AChE) inhibitor with analgesic, antipyretic, anti-inflammatory, neuroprotective, cardioprotective, and antioxidative activities. It has the CAS number 480-36-4 and is supplied for research purposes as a natural product standard. |
| Molecular Formula |
C28H32O14
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|---|---|
| Molecular Weight |
592.55
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| Exact Mass |
592.179
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| CAS # |
480-36-4
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| PubChem CID |
5317025
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
885.2±65.0 °C at 760 mmHg
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| Melting Point |
258-260ºC
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| Flash Point |
292.2±27.8 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.694
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| LogP |
2.1
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
42
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| Complexity |
955
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| Defined Atom Stereocenter Count |
10
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| SMILES |
C[C@H]1[C@@H]([C@H]([C@H]([C@@H](O1)OC[C@@H]2[C@H]([C@@H]([C@H]([C@@H](O2)OC3=CC(=C4C(=C3)OC(=CC4=O)C5=CC=C(C=C5)OC)O)O)O)O)O)O)O
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| InChi Key |
YFVGIJBUXMQFOF-PJOVQGMDSA-N
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| InChi Code |
InChI=1S/C28H32O14/c1-11-21(31)23(33)25(35)27(39-11)38-10-19-22(32)24(34)26(36)28(42-19)40-14-7-15(29)20-16(30)9-17(41-18(20)8-14)12-3-5-13(37-2)6-4-12/h3-9,11,19,21-29,31-36H,10H2,1-2H3/t11-,19+,21-,22+,23+,24-,25+,26+,27+,28+/m0/s1
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| Chemical Name |
5-hydroxy-2-(4-methoxyphenyl)-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one
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| Synonyms |
Linarin Buddleoside
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~421.91 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.51 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (3.51 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6876 mL | 8.4381 mL | 16.8762 mL | |
| 5 mM | 0.3375 mL | 1.6876 mL | 3.3752 mL | |
| 10 mM | 0.1688 mL | 0.8438 mL | 1.6876 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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