| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| Targets |
Licoflavone A targets protein tyrosine phosphatase-1B (PTP1B), an enzyme involved in insulin signaling and metabolic regulation. By inhibiting PTP1B with an IC50 of 54.5 μM, the compound modulates insulin signaling pathways. Licoflavone A may also target tyrosinase, as licorice flavonoids have been shown to have strong inhibitory effects on tyrosinase. The compound is a flavonoid from licorice root with potential applications in metabolic and cosmetic research.
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| ln Vitro |
In vitro, Licoflavone A inhibits protein tyrosine phosphatase-1B (PTP1B) with an IC50 of 54.5 μM. It is a flavonoid isolated from the roots of Glycyrrhiza uralensis. Compounds isolated from licorice roots including Licoflavone A showed significant PTP1B inhibitory activity in vitro. Licoflavone A has been shown to reduce the release of inflammatory factors in liver tissue, providing protection against LPS-induced acute hepatitis. It has strong inhibitory effects on tyrosinase.
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| ln Vivo |
In vivo, Licoflavone A has been shown to provide protection against LPS-induced acute hepatitis by reducing the release of inflammatory factors in liver tissue. As a PTP1B inhibitor, it may have potential for metabolic disorder research. The compound is a flavonoid from licorice root with potential applications in pharmacology and cosmetics. Further in vivo studies are needed to fully characterize its therapeutic potential.
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| Enzyme Assay |
In vitro enzyme assays for Licoflavone A involve measuring PTP1B inhibition. PTP1B activity is assessed using a suitable substrate, and the compound is added at varying concentrations. IC50 values are calculated from dose-response curves. For tyrosinase inhibition studies, tyrosinase activity is measured using L-DOPA as a substrate. Anti-inflammatory activity is assessed by measuring cytokine release in LPS-stimulated cells. Assays are performed in appropriate buffer systems with positive controls such as known PTP1B inhibitors.
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| Cell Assay |
In vitro cell-based assays for Licoflavone A are conducted in various cell lines. Cells are cultured in appropriate media at 37°C with 5% CO2 and treated with the compound at varying concentrations. PTP1B inhibition is assessed by measuring downstream signaling pathway activation. Anti-inflammatory activity is assessed by measuring cytokine production in LPS-stimulated cells. Cell viability is assessed by standard assays. Experiments are performed in triplicate with appropriate positive and negative controls.
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| Animal Protocol |
Licoflavone A in vivo studies are conducted in animal models of inflammation and metabolic disorders. Animals are treated with Licoflavone A via oral administration or injection. For anti-inflammatory studies, LPS-induced acute hepatitis models are used. Inflammatory cytokine levels in liver tissue are measured. For metabolic studies, glucose and insulin levels are assessed. Animals are monitored for clinical signs. Tissues and blood samples are collected for histopathological and biomarker analysis at study endpoints. Studies are conducted in accordance with institutional animal care guidelines.
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| ADME/Pharmacokinetics |
Licoflavone A (MW 322.35 g/mol, C20H18O4) is soluble in DMSO at 100 mg/mL (310.22 mM). It is practically insoluble in water (<0.1 mg/mL). The compound has a density of 1.279 g/cm3 (predicted). For in vivo administration, it can be formulated in 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% saline at 4 mg/mL (12.41 mM). Licoflavone A is a natural product isolated from the roots of Glycyrrhiza uralensis.
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| Toxicity/Toxicokinetics |
Licoflavone A is generally well-tolerated in preclinical studies. The compound is a natural flavonoid from licorice with established safety profiles. Its PTP1B inhibitory and anti-inflammatory activities have been demonstrated with acceptable safety profiles. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard safety precautions should be followed when handling. Comprehensive toxicological evaluation would be required for therapeutic development.
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| References | |
| Additional Infomation |
Licoflavone A is a type of flavonoid compound. It has been reported to exist in licorice (Glycyrrhiza uralensis), rough licorice (Glycyrrhiza aspera), and other organisms with relevant data. See also: Root (part) of inflated licorice (Glycyrrhiza inflata).
Licoflavone A is a flavonoid isolated from Glycyrrhiza uralensis roots that inhibits protein tyrosine phosphatase-1B (PTP1B) with an IC50 of 54.5 μM. It has anti-inflammatory effects and provides protection against LPS-induced acute hepatitis. The compound has strong inhibitory effects on tyrosinase and potential applications as a whitening agent. Its molecular formula is C20H18O4 with a molecular weight of 322.36 g/mol. All applications are limited to non-human research use. |
| Molecular Formula |
C20H18O4
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|---|---|
| Molecular Weight |
322.3545
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| Exact Mass |
322.121
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| CAS # |
61153-77-3
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| PubChem CID |
5319000
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| Appearance |
Off-white to yellow solid powder
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| Density |
1.279±0.06 g/cm3(Predicted)
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| Boiling Point |
551.1±50.0 °C(Predicted)
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| LogP |
4.379
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
24
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| Complexity |
524
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
HJGURBGBPIKRER-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H18O4/c1-12(2)3-4-14-9-16-18(23)11-19(24-20(16)10-17(14)22)13-5-7-15(21)8-6-13/h3,5-11,21-22H,4H2,1-2H3
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| Chemical Name |
7-hydroxy-2-(4-hydroxyphenyl)-6-(3-methylbut-2-enyl)chromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~310.22 mM)
H2O : ~1 mg/mL (~3.10 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.76 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.76 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1022 mL | 15.5111 mL | 31.0222 mL | |
| 5 mM | 0.6204 mL | 3.1022 mL | 6.2044 mL | |
| 10 mM | 0.3102 mL | 1.5511 mL | 3.1022 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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