Levetiracetam (UCB-L 059, SIB-S 1)

Alias: Levetiracetam, UCBL059, UCB L059, UCB-L059, SIB S1, SIBS1, SIB-S1, Keppra, Etiracetam, UCB6474, UCB-6474, UCB 6474,
Cat No.:V0335 Purity: ≥98%
Levetiracetam (also known as UCB-L059, SIB-S1)is a potent and selective M2 muscarinic acetylcholine receptors (mAChR) inhibitor andan anticonvulsant medication used to treat epilepsy.
Levetiracetam (UCB-L 059, SIB-S 1) Chemical Structure CAS No.: 102767-28-2
Product category: DNA Methyltransferase
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
50mg
250mg
500mg
1g
Other Sizes

Other Forms of Levetiracetam (UCB-L 059, SIB-S 1):

  • Etiracetam
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Levetiracetam (also known as UCB-L059, SIB-S1) is a potent and selective M2 muscarinic acetylcholine receptors (mAChR) inhibitor and an anticonvulsant medication used to treat epilepsy. Levetiracetam and related compounds bind to SV2A expressed in fibroblasts, indicating that SV2A is sufficient for Levetiracetam binding. Levetiracetam irreversibly inhibits the high-voltage-activated (HVA) calcium current by approximately 18% on the average in freshly isolated CA1 hippocampal neurons of rats. Levetiracetam selectively inhibits N-type Ca2+ channels of CA1 pyramidal hippocampal neurons.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Levetiracetam inhibits the activity of O6-methylguanine-DNA-methyltransferase (MGMT) by increasing HDAC transcription and enlisting corepressor complexes on the promoter [1]. Glioblastoma multiforme stem-like cells (GSC) are made more sensitive to temozolomide (250 μM) treatment by levetiracetam (40 μg/mL) [1]. Levetiracetam (40 μg/mL) treatment of GCSCs results in down-regulated MGMT expression[1].
ln Vivo
Levetiracetam (10, 25, or 50 mg/kg) suppresses EEG and behavioral seizure activity in neonates experiencing hypoxia [2].
Cell Assay
Cell Viability Assay[1]
Cell Types: GCSC neurospheres
Tested Concentrations: 40 μg/mL
Incubation Duration: 48 hrs (hours)
Experimental Results: Slight antitumor effect exerted by the treatment with Temozolomide (250 µM) or Levetiracetam (40 μg/mL) alone was strongly enhanced when Temozolomide and Levetiracetam were added in combination.

Western Blot Analysis[1]
Cell Types: Glioblastoma multiforme stem-like cells (GSCs)
Tested Concentrations: 40 μg/mL
Incubation Duration: 48 hrs (hours)
Experimental Results: A high level of MGMT expression in untreated GCSCs; this expression was slightly diminished after treatment with Temozolomide (250 µM) and Levetiracetam singularly but it was dramatically diminished after the combined treatment with Temozolomide and Levetiracetam.
Animal Protocol
Animal/Disease Models: Male Long-Evans rats[2]
Doses: 10, 25, or 50 mg/kg
Route of Administration: intraperitoneal (ip)injection 60 min before hypoxia.
Experimental Results: Treatment resulted in a significant decrease in hypoxic seizure (HS) duration at 25 mg/ kg and at 50 mg/kg. Anticonvulsant activity was maximal at 50 mg/kg, at which HSs were decreased by 63.6%.
References
[1]. Bianca Maria Scicchitano, et al. Levetiracetam enhances the temozolomide effect on glioblastoma stem cell proliferation and apoptosis. Cancer Cell Int. 2018 Sep 10;18:136.
[2]. Delia M Talos, et al. Antiepileptic effects of levetiracetam in a rodent neonatal seizure model. Pediatr Res. 2013 Jan;73(1):24-30.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C8H14N2O2
Molecular Weight
170.21
CAS #
102767-28-2
SMILES
O=C(N)[C@H](CC)N1C(CCC1)=O
InChi Key
HPHUVLMMVZITSG-LURJTMIESA-N
InChi Code
InChI=1S/C8H14N2O2/c1-2-6(8(9)12)10-5-3-4-7(10)11/h6H,2-5H2,1H3,(H2,9,12)/t6-/m0/s1
Chemical Name
(S)-2-(2-oxopyrrolidin-1-yl)butanamide
Synonyms
Levetiracetam, UCBL059, UCB L059, UCB-L059, SIB S1, SIBS1, SIB-S1, Keppra, Etiracetam, UCB6474, UCB-6474, UCB 6474,
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO:34 mg/mL (199.8 mM)
Water:34 mg/mL (199.8 mM)
Ethanol:34 mg/mL (199.8 mM)
Solubility (In Vivo)
Saline: 30 mg/mL
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 5.8751 mL 29.3755 mL 58.7510 mL
5 mM 1.1750 mL 5.8751 mL 11.7502 mL
10 mM 0.5875 mL 2.9375 mL 5.8751 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

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