| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg | |||
| Other Sizes |
| Targets |
Kaempferol 3-O-gentiobioside targets α-glucosidase with an IC50 of 25.3 ± 1.2 μM [1]
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|---|---|
| ln Vitro |
Kaempferol 3-O-gentiobioside (10 μM–100 μM) dose-dependently inhibited α-glucosidase activity. At 10 μM, the inhibition rate was 32.6% ± 2.1%; at 50 μM, it reached 78.4% ± 3.5%; and at 100 μM, the maximal inhibition rate was 91.2% ± 2.8% [1]
Kinetic analysis revealed that Kaempferol 3-O-gentiobioside acts as a competitive inhibitor of α-glucosidase, with an increased Km value (from 0.52 mM to 1.36 mM) and unchanged Vmax, indicating binding to the enzyme’s active site [1] |
| Enzyme Assay |
α-Glucosidase inhibition assay: α-Glucosidase enzyme was dissolved in buffer to prepare a working solution. Kaempferol 3-O-gentiobioside was prepared into serial concentrations (10 μM–100 μM) and mixed with the enzyme solution, then incubated at 37°C for 15 minutes. The substrate p-nitrophenyl-α-D-glucopyranoside (pNPG) was added to initiate the reaction, and incubation continued for 30 minutes at 37°C. The reaction was terminated by adding a basic solution, and the absorbance of the released p-nitrophenol was measured at 405 nm. The inhibition rate was calculated relative to the control group, and IC50 was obtained by fitting the dose-response curve. For kinetic analysis, the enzyme was incubated with fixed concentrations of the compound and varying concentrations of pNPG, and Lineweaver-Burk plots were generated to determine the inhibition type [1]
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| References | |
| Additional Infomation |
Kaempferol-3-β-gentiobiose is a kaempferol O-glucoside in which the hydroxyl hydrogen at the 3-position of kaempferol is replaced by a gentiobiose group. It is a metabolite of rapeseed. It is a disaccharide derivative, kaempferol O-glucoside and trihydroxyflavonoid. It is functionally related to gentiobiose. Kaempferol-3-gentiobiose has been reported to be found in Meconopsis grandis, soybean (Glycine max) and other organisms with relevant data. Kaempferol-3-O-gentiobiose is a natural flavonoid glycoside isolated from senna leaves (Cassia alata L.)[1]. Its antidiabetic effect is achieved by inhibiting α-glucosidase, an enzyme that catalyzes the hydrolysis of complex carbohydrates into glucose, thereby delaying the absorption of glucose in the intestine and reducing postprandial blood glucose rise[1]. As a competitive α-glucosidase inhibitor, kaempferol-3-O-gentiobiose has shown potential for development as a natural antidiabetic drug, particularly for the treatment of type 2 diabetes [1].
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| Molecular Formula |
C₂₇H₃₀O₁₆
|
|---|---|
| Molecular Weight |
610.52
|
| Exact Mass |
610.153
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| CAS # |
22149-35-5
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| PubChem CID |
9960512
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| Appearance |
White to yellow solid powder
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| Density |
1.8±0.1 g/cm3
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| Boiling Point |
991.0±65.0 °C at 760 mmHg
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| Flash Point |
328.0±27.8 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.764
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| LogP |
-0.52
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
16
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
43
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| Complexity |
1000
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| Defined Atom Stereocenter Count |
10
|
| SMILES |
C1=CC(=CC=C1C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O)O)O)O)O
|
| InChi Key |
BITPRCODIALMOV-DEFKTLOSSA-N
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| InChi Code |
InChI=1S/C27H30O16/c28-7-14-17(32)20(35)22(37)26(41-14)39-8-15-18(33)21(36)23(38)27(42-15)43-25-19(34)16-12(31)5-11(30)6-13(16)40-24(25)9-1-3-10(29)4-2-9/h1-6,14-15,17-18,20-23,26-33,35-38H,7-8H2/t14-,15-,17-,18-,20+,21+,22-,23-,26-,27+/m1/s1
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| Chemical Name |
5,7-dihydroxy-2-(4-hydroxyphenyl)-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one
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| Synonyms |
Kaempferol 3-O-gentiobioside Kaempferol 3-gentiobioside
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~81.90 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6379 mL | 8.1897 mL | 16.3795 mL | |
| 5 mM | 0.3276 mL | 1.6379 mL | 3.2759 mL | |
| 10 mM | 0.1638 mL | 0.8190 mL | 1.6379 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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