JNJ4796

Alias: JNJ-4796; JNJ4796; JNJ 4796
Cat No.:V4512 Purity: ≥98%
JNJ4796 is a novel, potent and oral bioactive fusion inhibitor of influenza virus, which neutralizes influenza A group 1 viruses by inhibiting hemagglutinin (HA)-mediated fusion.
JNJ4796 Chemical Structure CAS No.: 2241664-16-2
Product category: New7
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
1mg
Other Sizes
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Description: JNJ4796 is a novel, potent and oral bioactive fusion inhibitor of influenza virus, which neutralizes influenza A group 1 viruses by inhibiting hemagglutinin (HA)-mediated fusion. JNJ4796 was identified through structural knowledge of the interactions and mechanism of anti-stem bnAb CR6261. It mimics the functionality of the broadly neutralizing antibodies (bnAbs). Recent characterization of broadly neutralizing antibodies (bnAbs) against influenza virus identified the conserved hemagglutinin (HA) stem as a target for development of universal vaccines and therapeutics. Although several stem bnAbs are being evaluated in clinical trials, antibodies are generally unsuited for oral delivery.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Similar to bnAb CR6261, JNJ4796 was demonstrated to work by blocking the pH-sensitive conformational changes of HA, which in turn caused the viral and endosomal membranes to fuse and release the viral DNA into the host cell [1].
ln Vivo
Mice were protected against lethal challenge with 25 times the median lethal dose (LD50) of the H1N1 A/Puerto Rico/8/1934 virus by oral treatment of JNJ4796. In contrast to less than 50% survival with the less potent chemical JNJ8897, twice-daily dosages of JNJ4796 at 50 and 10 mg/kg, started the day before the challenge and continued for 7 days, resulted in 100% survival at day 21. 1]. After a sublethal viral challenge (LD90), oral dosages of JNJ4796 demonstrated dose-dependent effectiveness; at doses of 15 and 5 mg/kg twice daily, 100% survival was attained [1].
Animal Protocol
Animal/Disease Models: Female BALB/cAnNCrl mice were infected intranasally with 2 × 25 μL of 25 × LD50 or 1 × LD90 of H1N1 A/Puerto Rico/8/34[1 ]
Doses: 50 and 10 mg/kg. Directions: Take orally twice (two times) daily for 7 days.
Experimental Results: Day 21 survival was 100% compared to the less potent compound JNJ8897.
References
[1]. van Dongen MJP, et al. A small-molecule fusion inhibitor of influenza virus is orally active in mice. Science. 2019 Mar 8;363(6431).
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C28H27N9O3
Molecular Weight
537.572484254837
CAS #
2241664-16-2
SMILES
CC(NC1=CC=C(OC(C2=CC(C(N3CCN([C@@H](C4=NN(C)N=N4)C5=CC=CC=C5)CC3)=O)=NC=C2)=N6)C6=C1)=O
Synonyms
JNJ-4796; JNJ4796; JNJ 4796
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO : ~100 mg/mL (~186.02 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.8602 mL 9.3011 mL 18.6022 mL
5 mM 0.3720 mL 1.8602 mL 3.7204 mL
10 mM 0.1860 mL 0.9301 mL 1.8602 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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