Isoprenaline HCl (Isoproterenol HCl)

Alias: NCI-c55630; Isoprenaline hydrochloride; NCIc55630; NCI c55630; Isoproterenol

Cat No.:V1119 Purity: ≥98%

COA Product Data Instructions for use SDS

Isoprenaline HCL (NCIc55630; NCI c55630; Isoproterenol), the hydrochloride salt of Isoprenaline, is a non-selective beta-adrenergic receptor agonist that has been used for the treatment of bradycardia and heart block.

Isoprenaline HCl (Isoproterenol HCl) Chemical Structure CAS No.: 51-30-9
Product category: Adrenergic Receptor

This product is for research use only, not for human use. We do not sell to patients.

Size	Price	Stock	Qty
500mg
1g
2g
5g
10g
Other Sizes

ADD TO CART CHECKOUT BULK INQUIRY

1-708-310-1919 sales@invivochem.com

Other Forms of Isoprenaline HCl (Isoproterenol HCl):

Official Supplier of:

Top Publications Citing lnvivochem Products

Nature 2021, 597(7874):119-125.

Cell 2020,183:1202-1218.e25.

Science Adv 2022: 8, eabm9427.

Nature 597, 119–125 (2021)

Cell Stem Cell 2020,26(6):845-861.e12.

Science Trans Med 2023,15(701):eadd7872.

STTT 2023,8(1):91.

Cell Reports 2023,42(4):112313

Science Trans Med 2023, 15: eadd7872.

Cancer Cell 2021,39(4):529-547.e7.

Cancer Discov 2022,12(4):1106-1127.

Immunity 2023,56(3):620-634.e11.

J Am Chem Soc 2022,144:21831-21836.

Cell 2020,183:1202-1218.e25.

Science Adv 2022:8,eabm9427.

Nature 2021,597(7874):119-125.

Cell 2020,183:1202-1218.e25.

PNAS Nexus 2022,1(3):pgac063.

ACS Nano 2023,17(10):9110-9125.

Biomaterial 2023 Sep16:302:122333.

Purity & Quality Control Documentation

Current batch：

Purity: ≥98%

COA

MSDS

NMR

Instructions

Product Description
Bioactivity & Protocols
Physicochemical Properties
Solubility Data
Calculators
Clinical Trial Information

Product Description

Biological Activity I Assay Protocols (From Reference)

Targets

β adrenergic receptor

ln Vitro

In vitro activity: Isoprenaline (Isoproterenol) hydrochloride (300 nM, 3 min) increases in intact rat fat cells the low-Km cAMP phosphodiesterase (cAMP-PDE) activity that is inhibited by particulate cGMP and cilostamide by approximately 100%[1].
Isoprenaline suppresses insulin-stimulated glucose transport activity in rat adipocytes. In the absence of adenosine, isoprenaline causes a time-dependent (t1/2 of about 2 min) decrease in the accessibility of GLUT4 on the cell surface that is stimulated by insulin that is greater than 50%. This decrease is directly correlated with the observed inhibition of transport activity[2].
Isoprenaline (5 nM and 10 μM) raises the levels of cyclic AMP; this effect is enhanced by cyclic GMP-elevating agents (50 nM ANF or 30 nM SNP plus 100 nM DMPPO), cilostamide (10 mM), and rolipram, a cyclic AMP-specific PDE (PDE 4) inhibitor (10 mM)[3].
Isoprenaline causes the Gi alpha-2 gene's transcriptional activity to rise to 140% of the control value, while Gs alpha gene specific hybridization stays the same[4].
Isoprenaline (20 nM) raises the total iK amplitude and, both in the absence and in the presence of 300 nM nisoldipine to block the L-type Ca2+ current, results in a negative shift of roughly 10 mV in the iK activation curve[5].
Isoprenaline (20 nM) raises the rate of spontaneous pacemaker occurrence of sino-atrial node pacemaker cells in isolated rabbit pacemaker cells by sixteen percent[5].

ln Vivo

Isoprenaline (also known as isoproterenol) hydrochloride (oral, 0.27–0.64 μg/kg) is extensively metabolized through a limited number of reactions in dogs[6].

Animal Protocol

Dogs
0.27-0. 64 μg/kg
oral

References

[1]. Evidence that insulin and isoprenaline activate the cGMP-inhibited low-Km cAMP phosphodiesterase in rat fat cells by phosphorylation. Proc Natl Acad Sci U S A. 1990 Jan;87(2):533-7.

[2]. Cell surface accessibility of GLUT4 glucose transporters in insulin-stimulated rat adipose cells. Modulation by isoprenaline and adenosine. Biochem J. 1992 Nov 15;288 (Pt 1):325-30.

[3]. Effects of cyclic GMP elevation on isoprenaline-induced increase in cyclic AMP and relaxation in rat aortic smooth muscle: role of phosphodiesterase 3. Br J Pharmacol. 1996 Oct;119(3):471-8.

[4]. Isoprenaline stimulates gene transcription of the inhibitory G protein alpha-subunit Gi alpha-2 in rat heart. Circ Res. 1993 Mar;72(3):696-700.

[5]. Modulation of delayed rectifier potassium current, iK, by isoprenaline in rabbit isolated pacemaker cells. Exp Physiol. 2000 Jan;85(1):27-35.

[6]. Metabolism of isoprenaline in dog and man. Br J Pharmacol . 1972 Nov;46(3):458-72.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Physicochemical Properties

Molecular Formula

C11H18CLNO3

Molecular Weight

247.72

Exact Mass

247.1

Elemental Analysis

C, 53.34; H, 7.32; Cl, 14.31; N, 5.65; O, 19.38

CAS #

51-30-9

Related CAS #

Isoprenaline; 7683-59-2; Isoprenaline hemisulfate; 299-95-6

Appearance

Solid powder

SMILES

CC(C)NCC(C1=CC(=C(C=C1)O)O)O.Cl

InChi Key

IROWCYIEJAOFOW-UHFFFAOYSA-N

InChi Code

InChI=1S/C11H17NO3.ClH/c1-7(2)12-6-11(15)8-3-4-9(13)10(14)5-8;/h3-5,7,11-15H,6H2,1-2H3;1H

Chemical Name

4-[1-hydroxy-2-(propan-2-ylamino)ethyl]benzene-1,2-diol;hydrochloride

Synonyms

NCI-c55630; Isoprenaline hydrochloride; NCIc55630; NCI c55630; Isoproterenol

HS Tariff Code

2934.99.03.00

Storage

Powder -20°C 3 years

4°C 2 years

In solvent -80°C 6 months

-20°C 1 month

Shipping Condition

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Solubility Data

Solubility (In Vitro)

DMSO: 50~80 mg/mL (201.8~323 mM)

Water: ~50 mg/mL (~201.8 mM)

Ethanol: <1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (8.40 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.

Solubility in Formulation 2: ≥ 2.08 mg/mL (8.40 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.

View More

Solubility in Formulation 3: ≥ 2.08 mg/mL (8.40 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.

Solubility in Formulation 4: ~2.1 mg/mL or 8.40 mM in 10% DMSO: 40% PEG300: 5% Tween-80: 45% Saline
~2.1 mg/mL or 8.40 mM in 10% DMSO: 90% (20% SBE-β-CD in Saline)

Solubility in Formulation 5: 100 mg/mL (403.68 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.

(Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.0368 mL	20.1841 mL	40.3682 mL
	5 mM	0.8074 mL	4.0368 mL	8.0736 mL
	10 mM	0.4037 mL	2.0184 mL	4.0368 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Mass

Concentration

Volume

Molecular Weight*

Calculate Reset

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

Calculate the Mass of a compound required to prepare a solution of known volume and concentration
Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

Enter 350.26 in the Molecular Weight (MW) box
Enter 10 in the Concentration box and choose the correct unit (mM)
Enter 5 in the Volume box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculate Reset

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
Enter 25 into the Concentration (End) box and select the correct unit (mM)
Enter 25 into the Volume (End) box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box

Molecular formula

Total molecular weight

g/mol

Calculate Reset

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4 c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate Reset

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
Click the “Calculate” button
The answer appears in the Volume (to add to vial) box

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)

Dosage mg/kg

Average weight of animals g

Dosing volume per animal μL

Number of animals

Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)

% DMSO

% Tween 80

% ddH₂O

Calculate Reset

Calculation results

Working concentration： mg/mL；

Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O，mix and clarify.

Note： (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.

Clinical Trial Information

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05219799	Recruiting	Drug: Propranolol Hydrochloride Drug: Isoproterenol	Obesity Vasodilation Healthy	University of Missouri-Columbia	March 14, 2023	Early Phase 1
NCT05997732	Recruiting	Drug: Phenylephrine Hydrochloride Drug: Isoproterenol Hydrochloride	Vasoconstriction Vasodilation	University of Alberta	October 31, 2023	Phase 4