| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| ln Vitro |
At a concentration of 25.0 µM, isomucronulitol showed moderate inhibitory activity on LPS-stimulated IL-12 p40 production in bone marrow-derived dendritic cells (BMDCs), as assessed by the inhibition rate compared to vehicle-treated DCs. It exhibited weaker activity than compounds 1 (isoliquiritigenin) and 2 (liquiritigenin) but stronger activity than its glucoside derivative (compound 12) [1].
The compound did not exhibit cytotoxicity in BMDCs at the tested concentration (25.0 µM) as evaluated by a colorimetric MTT assay [1]. |
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| Cell Assay |
Bone marrow-derived dendritic cells (BMDCs) were obtained from wild-type C57BL/6 mice. Mouse tibia and femur were flushed with culture medium to collect bone marrow cells. Cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 50 µM β-mercaptoethanol, 2 mM glutamine, and 3% J558L hybridoma cell culture supernatant containing granulocyte-macrophage colony-stimulating factor. Fresh medium was replaced every second day. On day 6, non-adherent cells and loosely adherent DC aggregates were harvested, washed, and resuspended in RPMI 1640 with 5% fetal bovine serum [1].
For cytokine production measurement, BMDCs were seeded in 48-well plates at 1×10⁵ cells per well in 0.5 mL medium and treated with isomucronulitol at a concentration of 25.0 µM for 1 hour prior to stimulation with 10 ng/mL lipopolysaccharide (LPS) from Salmonella minnesota. Supernatants were collected 18 hours after stimulation. Concentrations of murine IL-12 p40 in the culture supernatants were determined by ELISA. The inhibitory effect on IL-12 p40 production was expressed as inhibition rate (%) relative to vehicle-treated DCs [1]. Cell viability of BMDCs treated with isomucronulitol at 25.0 µM was evaluated using a colorimetric MTT assay, which confirmed no cytotoxicity at the tested concentration [1]. |
| Toxicity/Toxicokinetics |
isomucronulitol showed no cytotoxicity in bone marrow-derived dendritic cells at a concentration of 25.0 µM as determined by MTT assay [1].
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| References | |
| Additional Infomation |
Isomucronulatol is an ether compound belonging to the flavonoid class. It has been reported that Isomucronulatol is found in black locust, acacia, and other organisms with relevant data.
isomucronulitol (compound 11) is an isoflavonoid skeleton compound isolated from Astragalus membranaceus roots. Its structure was elucidated by comparing spectroscopic data with reported values. In the biological evaluation, isoflavonoid skeletons (including compound 11) showed stronger inhibitory effects on LPS-induced IL-12 p40 production than their corresponding glucosides (compound 12) [1]. |
| Molecular Formula |
C17H18O5
|
|---|---|
| Molecular Weight |
302.3218
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| Exact Mass |
302.115
|
| CAS # |
52250-35-8
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| Related CAS # |
(R)-Isomucronulatol; 64474-51-7
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| PubChem CID |
602152
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| Appearance |
White to off-white solid
|
| Density |
1.3±0.1 g/cm3
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| Boiling Point |
426.8±45.0 °C at 760 mmHg
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| Melting Point |
152 - 153 °C
|
| Flash Point |
211.9±28.7 °C
|
| Vapour Pressure |
0.0±1.1 mmHg at 25°C
|
| Index of Refraction |
1.607
|
| LogP |
2.63
|
| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
363
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
O1C2C([H])=C(C([H])=C([H])C=2C([H])([H])C([H])(C2C([H])=C([H])C(=C(C=2O[H])OC([H])([H])[H])OC([H])([H])[H])C1([H])[H])O[H]
|
| InChi Key |
NQRBAPDEZYMKFL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H18O5/c1-20-14-6-5-13(16(19)17(14)21-2)11-7-10-3-4-12(18)8-15(10)22-9-11/h3-6,8,11,18-19H,7,9H2,1-2H3
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| Chemical Name |
3-(2-hydroxy-3,4-dimethoxyphenyl)-3,4-dihydro-2H-chromen-7-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~330.78 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.27 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.27 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.27 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3078 mL | 16.5388 mL | 33.0775 mL | |
| 5 mM | 0.6616 mL | 3.3078 mL | 6.6155 mL | |
| 10 mM | 0.3308 mL | 1.6539 mL | 3.3078 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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