| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
The specific molecular target of isobutyryl-L-carnitine is not clearly defined. It is an endogenous metabolite produced by the action of acyl-CoA dehydrogenases. It is involved in fatty acid oxidation and organic acid metabolism. It is used as a biomarker for metabolic disorders. Further research is needed to identify any specific biological targets or signaling functions.
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|---|---|
| ln Vitro |
Isobutyryl-L-carnitine is a natural 4-carbon acylcarnitine that is involved in fatty acid oxidation and organic acid metabolism. It is the product of the acyl-CoA dehydrogenases. Specific IC50 or EC50 values are not applicable as it is an endogenous metabolite.
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| ln Vivo |
In vivo, isobutyryl-L-carnitine is a metabolite found in human blood and urine. Its levels are altered in certain metabolic disorders. It is used as a biomarker for the diagnosis and monitoring of organic acidemias and fatty acid oxidation defects. No specific in vivo efficacy studies have been reported for this endogenous metabolite.
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| Enzyme Assay |
Non-cell assays for isobutyryl-L-carnitine include quantification in blood or urine by tandem mass spectrometry (MS/MS) or HPLC. The metabolite is extracted and analyzed against calibration standards.
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| Cell Assay |
Not applicable. Isobutyryl-L-carnitine is an endogenous metabolite used as a biomarker, not in cell-based assays.
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| Animal Protocol |
Not applicable. Isobutyryl-L-carnitine is an endogenous metabolite used as a biomarker, not in animal experiments.
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| References | |
| Additional Infomation |
O-Isobutyryl-L-carnitine is the optically active form of O-isobutyrylcarnitine with the (R)-configuration. It is a human metabolite. It is an O-isobutyrylcarnitine, methyl branched fatty acyl-L-carnitine, saturated fatty acyl-L-carnitine, and short-chain fatty acyl-L-carnitine.
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| Molecular Formula |
C11H21NO4
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|---|---|
| Molecular Weight |
231.28874
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| Exact Mass |
231.147
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| CAS # |
25518-49-4
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| Related CAS # |
Isobutyryl-L-carnitine chloride;6920-31-6
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| PubChem CID |
168379
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| Appearance |
White to off-white solid
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| Source |
Endogenously produced metabolite
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| LogP |
1.4
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
16
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| Complexity |
250
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(C)C(O[C@H](CC([O-])=O)C[N+](C)(C)C)=O
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| InChi Key |
LRCNOZRCYBNMEP-SECBINFHSA-N
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| InChi Code |
InChI=1S/C11H21NO4/c1-8(2)11(15)16-9(6-10(13)14)7-12(3,4)5/h8-9H,6-7H2,1-5H3/t9-/m1/s1
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| Chemical Name |
(3R)-3-(2-methylpropanoyloxy)-4-(trimethylazaniumyl)butanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~1080.89 mM)
H2O : ~125 mg/mL (~540.45 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (8.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (8.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (8.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3236 mL | 21.6179 mL | 43.2358 mL | |
| 5 mM | 0.8647 mL | 4.3236 mL | 8.6472 mL | |
| 10 mM | 0.4324 mL | 2.1618 mL | 4.3236 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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