| Size | Price | |
|---|---|---|
| 10mg | ||
| 25mg | ||
| 50mg | ||
| 100mg | ||
| 250mg |
Purity: ≥98%
Iberin (NSC 321801), a sulfoxide analog of sulforaphane, is a naturally occurring antioxidant that is a member of isothiocyanate family. It inhibits cell survival with an IC50 of 2.3 μM in HL60 cell. It induces phase II enzyme activity and suppresses CDK expression, inducing apoptosis in cancer cells.
| ln Vitro |
Neuroblastoma cell proliferation is inhibited by iberin in a time- and dose-dependent manner. In neuroblastoma cells, iberin-induced cell cycle arrest is linked to suppression of cyclin-dependent kinase Cdk2, Cdk4, and Cdk6 protein expression. Ibelin-treated cells showed a higher rate of apoptotic cell death when compared to control cells. Research has revealed a connection between the activation of PARP, caspase-3, and caspase-9 and iberin-induced apoptosis [2]. Iberin increases cytotoxicity, slows the growth of human glioblastoma cells in tests of cell proliferation, and triggers apoptosis by activating caspase-3 and caspase-9 [3].
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| ln Vivo |
Iberin increases tissue levels of the phase II detoxification enzymes quinone reductase and glutathione S-transferase in various rat tissues [5]. Iberin was tested in an in vivo mouse model of foreign body infection, and the results showed no significant difference in bacterial clearance between treated and untreated mice [4].
|
| References |
[1]. Jakubikova J, et al. Isothiocyanates induce cell cycle arrest, apoptosis and mitochondrial potential depolarization in HL-60 and multidrug-resistant cell lines. Anticancer Res. 2005 Sep-Oct;25(5):3375-86.
[2]. Jadhav U, et al. Iberin induces cell cycle arrest and apoptosis in human neuroblastoma cells. [3]. Jadhav U, et al. Dietary isothiocyanate iberin inhibits growth and induces apoptosis in human glioblastoma cells. J Pharmacol Sci. 2007 Feb;103(2):247-51. [4]. Jakobsen TH, et al. Food as a source for quorum sensing inhibitors: iberin from horseradish revealed as a quorumsensing inhibitor of Pseudomonas aeruginosa. Appl Environ Microbiol. 2012 Apr;78(7):2410-21. [5]. Munday R, et al. Induction of phase II detoxification enzymes in rats by plant-derived isothiocyanates: comparison of allyl isothiocyanate with sulforaphane and related compounds. J Agric Food Chem. 2004 Apr 7;52(7):1867-71 |
| Molecular Formula |
C5H9NOS2
|
|---|---|
| Molecular Weight |
163.26106
|
| CAS # |
505-44-2
|
| SMILES |
S=C=NCCCS(C)=O
|
| InChi Key |
LELAOEBVZLPXAZ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C5H9NOS2/c1-9(7)4-2-3-6-5-8/h2-4H2,1H3
|
| Chemical Name |
1-isothiocyanato-3-(methylsulfinyl)propane
|
| Synonyms |
NSC-321801; NSC321801; NSC 321801; 1-Isothiocyanato-3-(methylsulfinyl)propane; 3-Methylsulfinylpropyl isothiocyanate; 3-Methylsulphinylpropylisothiocyanate; Iberin
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~612.52 mM)
Ethanol : ~10 mg/mL (~61.25 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (15.31 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (15.31 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (15.31 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.1252 mL | 30.6260 mL | 61.2520 mL | |
| 5 mM | 1.2250 mL | 6.1252 mL | 12.2504 mL | |
| 10 mM | 0.6125 mL | 3.0626 mL | 6.1252 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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