| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| ln Vitro |
Hydroxytyrosol acetate showed DPPH radical scavenging activity with an EC50 (concentration required for 50% reduction of DPPH radical) of 0.26 mol/mol at 15 min, 0.22 mol/mol at 60 min, and 0.14 mol/mol at 250 min; the number of reduced DPPH radicals per molecule at these times were 2.0, 2.2, and 3.6, respectively. Its EC50 at 15 min was significantly higher (weaker activity) than that of hydroxytyrosol (0.19), oleuropein (0.22), and 3,4-DHPEA-EA (0.12), but similar to α-tocopherol (0.25) and trolox (0.24). The EC50 of Hydroxytyrosol acetate decreased by 46% between 15 and 250 min, indicating slower reactions with the radical. [1]
In stripped olive oil (0.3 mmol/kg additive) stored at 60°C, Hydroxytyrosol acetate extended the time to reach a peroxide value (PV) of 50 meq/kg to 66.54 days (mean), which was significantly longer than α-tocopherol (20.24 days) and oleuropein (8.51 days), and comparable to phenolic extract (66.03 days), 3,4-DHPEA-EA (64.38 days), and hydroxytyrosol (70.45 days). The anisidine value (AV) determination confirmed a similar order of antioxidant activity. [1] In 30% oil-in-water emulsions (0.3 mmol/kg additive) at pH 5.5 (acetate buffer, 60°C), Hydroxytyrosol acetate gave a time to reach a conjugated diene content of 0.4% of 7.78 days, which was significantly longer than hydroxytyrosol (5.99 days) and the control (3.79 days), but shorter than α-tocopherol (14.14 days) and 3,4-DHPEA-EA (13.40 days); it was similar to oleuropein (7.94 days) and phenolic extract (8.38 days). At pH 7.4 in phosphate buffer, the time to 0.4% conjugated dienes was 7.84 days for Hydroxytyrosol acetate, significantly longer than hydroxytyrosol (6.57 days) and oleuropein (7.02 days), but shorter than α-tocopherol (12.04 days) and 3,4-DHPEA-EA (8.13 days). In MOPS buffer at pH 7.4, the time was 17.65 days, similar to hydroxytyrosol (16.97 days) and α-tocopherol (21.21 days), but shorter than oleuropein (20.48 days) and 3,4-DHPEA-EA (22.58 days). The anisidine value determinations confirmed these orders of activity. [1] The partition coefficient (log P) of Hydroxytyrosol acetate was 0.82 (±0.01) at both pH 5.5 and pH 7.4, indicating moderate lipophilicity. [1] |
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| References | |
| Additional Infomation |
Reports have indicated that olives contain hydroxytyrosol acetate, and relevant data is available for reference.
Hydroxytyrosol acetate is a hydroxytyrosol derivative found in olive oil. Its acetate group may hinder the scavenging effect of the hydroxyl groups by intra- or intermolecular hydrogen bonding, contributing to reduced radical scavenging activity in the DPPH test. However, in bulk oil, van der Waals interactions between triglyceride molecules and the ester side chain may prevent such hydrogen bonding, making its antioxidant activity similar to that of hydroxytyrosol. In emulsions, the polar paradox explains that less polar antioxidants (like Hydroxytyrosol acetate) can be more effective in more polar media; it was slightly more effective than hydroxytyrosol at pH 5.5 but not at pH 7.4. Overall, Hydroxytyrosol acetate and hydroxytyrosol have similar antioxidant activity in oil and emulsions, despite differences in DPPH radical scavenging. [1] |
| Molecular Formula |
C10H12O4
|
|---|---|
| Molecular Weight |
196.1999
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| Exact Mass |
196.073
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| CAS # |
69039-02-7
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| PubChem CID |
155240
|
| Appearance |
White to off-white solid
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
355.9±27.0 °C at 760 mmHg
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| Flash Point |
141.6±17.2 °C
|
| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.564
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| LogP |
0.96
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
14
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| Complexity |
193
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C(C([H])([H])[H])=O)C([H])([H])C([H])([H])C1C([H])=C([H])C(=C(C=1[H])O[H])O[H]
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| InChi Key |
FGJGLFPNIZXRLV-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H12O4/c1-7(11)14-5-4-8-2-3-9(12)10(13)6-8/h2-3,6,12-13H,4-5H2,1H3
|
| Chemical Name |
2-(3,4-dihydroxyphenyl)ethyl acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~1274.21 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 6.25 mg/mL (31.86 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 6.25 mg/mL (31.86 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 6.25 mg/mL (31.86 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0968 mL | 25.4842 mL | 50.9684 mL | |
| 5 mM | 1.0194 mL | 5.0968 mL | 10.1937 mL | |
| 10 mM | 0.5097 mL | 2.5484 mL | 5.0968 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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