| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| 2g |
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| 5g | |||
| Other Sizes |
| Targets |
Endogenous Metabolite
Hyaluronic acid targets multiple pathways. It activates the PI3K-Akt signaling pathway and enhances proteolytic MMP-9 binding. High molecular weight HA functions as a barrier to the inflammatory process, lessening the chemotaxis and migration of inflammatory cells, and guarding against the damaging effects of free radicals. It also serves as a ligand for cell surface receptors like CD44. |
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| ln Vitro |
Hyaluronic acid (HA) is frequently utilized in cosmetic medicine because of its capacity to attach to a significant number of water molecules. Tissue hydration and resistance to mechanical injury are both enhanced by it. The processes of wound healing, ovulation, fertilization, signal transduction, and tumor physiology are all significantly impacted by HA. Rheumatoid arthritis and osteoarthritis are two joint conditions that are treated with HA. High molecular weight hyaluronic acid functions as a strong barrier to the inflammatory process, lessens the chemotaxis and migration of inflammatory cells, and guards against the damaging effects of free radicals. Because of its ability to lubricate the corneal endothelium, HA is utilized in ophthalmology. It is also used in cosmetic dermatology to increase tissue hydration and cellular resilience to mechanical damage, all while having minimal negative effects. Numerous studies have demonstrated its function in liver disorders, medications, and tumor indicators [1]. The growth and metastasis of cancer are significantly influenced by hyaluronic acid. In addition to promoting cell proliferation, adhesion, migration, and invasion, HA and HA fragment-tumor cell interactions can also induce angiogenesis, lymphangiogenesis, the epithelial-mesenchymal transition, stem cell-like properties, and resistance to chemotherapy and radiation in digestive tract tumors. therapeutic qualities[2].
In vitro, Hyaluronic acid demonstrates several biological activities. It enhances cell invasion and angiogenesis by promoting MMP-9 binding. It improves tissue hydration and their resistance to mechanical damage. Low molecular weight HA can promote inflammation, while high molecular weight HA has anti-inflammatory effects. |
| ln Vivo |
Numerous studies on animals have shown the benefits of intra-articular hyaluronic acid administration. Studies on HA reveal that it can increase the synthesis of cartilage matrix, stop it from degrading, lessen inflammation, trigger the production of endogenous HA, and enhance the cartilage's suppleness and moisture content [1]. Fresh wounds can heal more quickly when polymer HA formulations are applied topically. They can also be utilized to treat chronic wounds and aid in the healing of venous leg ulcers [3].
In vivo, Hyaluronic acid is used for the treatment of joint diseases, such as osteoarthritis, through intra-articular injection. It is also used in ophthalmology during surgery and to treat dry eyes. It is used as a dermal filler in aesthetic medicine. It plays an important role in wound healing, ovulation, and fertilization. |
| Enzyme Assay |
Hyaluronic acid (hyaluronan, HA) is a linear polysaccharide formed from disaccharide units containing N-acetyl-D-glucosamine and glucuronic acid. It has a high molecular mass, usually in the order of millions of Daltons, and interesting viscoelastic properties influenced by its polymeric and polyelectrolyte characteristics. HA is present in almost all biological fluids and tissues. In clinical medicine, it is used as a diagnostic marker for many diseases including cancer, rheumatoid arthritis and liver pathologies, as well as for supplementation of impaired synovial fluid in arthritic patients by means of intra-articular injections. It is also used in certain ophthalmological and otological surgeries and cosmetic regeneration and reconstruction of soft tissue[3].
The in vitro assay for Hyaluronic acid is not a standard enzyme inhibition assay. Its activity is often assessed in cell-based models. For example, its effect on cell migration is measured using a scratch assay. Its ability to bind to CD44 is measured using an ELISA-based binding assay. Its molecular weight is determined by gel permeation chromatography (GPC). |
| Cell Assay |
Hyaluronan (HA), an extracellular and peri-cellular glycosaminoglycan with a large molecular weight, plays an important role in cancer growth and metastasis. The correlation between HA accumulation and tumor progression has been shown in various digestive cancers. HA and HA fragment-tumor cell interaction could activate the downstream signaling pathways, promoting cell proliferation, adhesion, migration and invasion, and inducing angiogenesis, lymphangiogenesis, epithelial-mesenchymal transition, stem cell-like property, and chemoradioresistance in digestive cancers.[2]
In vitro cell culture studies for Hyaluronic acid utilize various cell types, including chondrocytes, synoviocytes, and fibroblasts. Cells are treated with the compound, and cell proliferation, migration, and the production of inflammatory mediators are assessed. Its effect on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) is also studied. |
| Animal Protocol |
In vivo animal experiments for Hyaluronic acid are performed in models of osteoarthritis, such as the MIA model in rats. The compound is administered via intra-articular injection, and its effect on cartilage degeneration is assessed by histological scoring. Wound healing models are also used to study its effect on tissue repair.
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| ADME/Pharmacokinetics |
Hyaluronic acid is a high molecular weight polysaccharide with a molecular weight ranging from 1,000 to 10,000,000 Da. It is a hygroscopic powder that is highly soluble in water. It is typically stored as a powder at 4°C or -20°C, protected from moisture. Its stability is maintained under recommended storage conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for Hyaluronic acid indicate that it is generally well-tolerated and has a very low potential for toxicity. As a naturally occurring substance, it is biocompatible and non-immunogenic. It is not considered carcinogenic or teratogenic. It is not intended for systemic therapeutic use and is typically administered locally.
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| References | |
| Additional Infomation |
Hyaluronic acid is approved by the FDA for various medical applications. It is approved as a medical device for the treatment of osteoarthritis (viscosupplementation) and for use in ophthalmic surgery. It is also approved as a dermal filler for cosmetic use. It is available in oral, topical, and injectable forms. It is not approved as a drug for systemic use.
|
| Molecular Formula |
C28H44N2O23
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|---|---|
| Molecular Weight |
5000-130000
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| Exact Mass |
776.233
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| CAS # |
9004-61-9
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| Related CAS # |
Hyaluronic acid sodium;9067-32-7
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| PubChem CID |
24728612
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| Appearance |
White to off-white solid powder
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| Density |
1.8±0.1 g/cm3
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| Boiling Point |
1274.4±65.0 °C at 760 mmHg
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| Flash Point |
724.5±34.3 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.666
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| LogP |
-6.62
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| Hydrogen Bond Donor Count |
14
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| Hydrogen Bond Acceptor Count |
23
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
53
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| Complexity |
1300
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| Defined Atom Stereocenter Count |
16
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| SMILES |
CC(=O)N[C@@H]1[C@H]([C@@H]([C@H](OC1O)CO)O)O[C@H]2[C@@H]([C@H]([C@@H](C(O2)C(=O)O)O[C@H]3[C@@H](C([C@@H]([C@H](O3)CO)O)O[C@@H]4[C@@H]([C@H](C([C@H](O4)C(=O)O)O)O)O)NC(=O)C)O)O
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| InChi Key |
KIUKXJAPPMFGSW-YXBJCWEESA-N
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| InChi Code |
InChI=1S/C28H44N2O23/c1-5(33)29-9-18(11(35)7(3-31)47-25(9)46)49-28-17(41)15(39)20(22(53-28)24(44)45)51-26-10(30-6(2)34)19(12(36)8(4-32)48-26)50-27-16(40)13(37)14(38)21(52-27)23(42)43/h7-22,25-28,31-32,35-41,46H,3-4H2,1-2H3,(H,29,33)(H,30,34)(H,42,43)(H,44,45)/t7-,8-,9-,10-,11-,12-,13+,14?,15-,16-,17-,18-,19?,20+,21+,22?,25?,26+,27+,28-/m1/s1
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| Chemical Name |
(2S,4S,5R,6S)-6-[(2S,3R,5S,6R)-3-acetamido-2-[(3S,4R,5R,6R)-6-[(3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid
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| Synonyms |
Hyaluronan; Hyaluronate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~14.29 mg/mL
DMSO : ~1 mg/mL) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 9.09 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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