| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
Purity: ≥98%
| Targets |
Endogenous Metabolite
Sodium hyaluronate binds to cell surface proteins, including CD44 and RHAMM, and activates signaling pathways such as PI3K-Akt. It also promotes the synthesis of cartilage matrix, prevents its degradation, reduces inflammation, and stimulates the synthesis of endogenous HA. It acts as a regulator of cancer-associated lymphangiogenesis and enhances cell invasion and angiogenesis by promoting proteolytic MMP-9 binding to the cell surface. |
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| ln Vitro |
Because hyaluronic acid (HA) can connect to a lot of water molecules, it is utilized a lot in aesthetic treatment. Tissue hydration and resistance to mechanical injury are both enhanced by it. The processes of wound healing, ovulation, fertilization, signal transduction, and tumor physiology are all significantly impacted by HA. Rheumatoid arthritis and osteoarthritis are two joint conditions that are treated with HA. High molecular weight hyaluronic acid functions as a strong barrier to the inflammatory process, lessens the chemotaxis and migration of inflammatory cells, and guards against the damaging effects of free radicals. Because of its ability to lubricate the corneal endothelium, HA is utilized in ophthalmology. It is also used in cosmetic dermatology to increase tissue hydration and cellular resilience to mechanical damage, all while having minimal negative effects. Numerous studies have demonstrated its function in liver disorders, medications, and tumor indicators [1]. The growth and metastasis of cancer are significantly influenced by hyaluronic acid. In addition to promoting cell proliferation, adhesion, migration, and invasion, HA and HA fragment-tumor cell interactions can also induce angiogenesis, lymphangiogenesis, the epithelial-mesenchymal transition, stem cell-like properties, and resistance to chemotherapy and radiation in digestive tract tumors. therapeutic qualities[2].
Sodium hyaluronate promotes the synthesis of cartilage matrix and prevents its degradation in vitro. It reduces inflammation and stimulates the synthesis of endogenous HA. It activates the PI3K-Akt signaling pathway and enhances cell invasion and angiogenesis by promoting MMP-9 binding to the cell surface. Its effects on cell proliferation and migration have been demonstrated in various cell culture models. |
| ln Vivo |
Numerous studies on animals have shown the benefits of intra-articular hyaluronic acid administration. Studies on HA reveal that it can increase the synthesis of cartilage matrix, stop it from degrading, lessen inflammation, trigger the production of endogenous HA, and enhance the cartilage's suppleness and moisture content [1]. Fresh wounds can heal more quickly when polymer HA formulations are applied topically. They can also be utilized to treat chronic wounds and aid in the healing of venous leg ulcers [3].
Sodium hyaluronate improves tissue hydration and resistance to mechanical damage in vivo. It functions as a lubricant and cushioning agent in joints, enhancing flexibility and shock-absorbing qualities. It is used in ophthalmology as a viscoelastic agent during surgery. It promotes wound healing and prevents skin aging. It also serves as a drug delivery system to enhance the efficacy of therapeutics. |
| Enzyme Assay |
Hyaluronic acid (hyaluronan, HA) is a linear polysaccharide formed from disaccharide units containing N-acetyl-D-glucosamine and glucuronic acid. It has a high molecular mass, usually in the order of millions of Daltons, and interesting viscoelastic properties influenced by its polymeric and polyelectrolyte characteristics. HA is present in almost all biological fluids and tissues. In clinical medicine, it is used as a diagnostic marker for many diseases including cancer, rheumatoid arthritis and liver pathologies, as well as for supplementation of impaired synovial fluid in arthritic patients by means of intra-articular injections. It is also used in certain ophthalmological and otological surgeries and cosmetic regeneration and reconstruction of soft tissue[3].
Hyaluronate Sodium does not have a typical enzyme/receptor binding assay. Its activity is assessed based on its physical and chemical properties, such as viscosity, molecular weight, and purity. Its binding to cell surface receptors, such as CD44, can be assessed using radioligand binding or surface plasmon resonance. Its effects on signaling pathways can be assessed in cell-based assays. |
| Cell Assay |
Hyaluronan (HA), an extracellular and peri-cellular glycosaminoglycan with a large molecular weight, plays an important role in cancer growth and metastasis. The correlation between HA accumulation and tumor progression has been shown in various digestive cancers. HA and HA fragment-tumor cell interaction could activate the downstream signaling pathways, promoting cell proliferation, adhesion, migration and invasion, and inducing angiogenesis, lymphangiogenesis, epithelial-mesenchymal transition, stem cell-like property, and chemoradioresistance in digestive cancers.[2]
In vitro cellular experiments for Sodium hyaluronate involve treating various cell types, including chondrocytes, fibroblasts, and cancer cells, with the compound. Its effects on cell proliferation, migration, and signaling pathways are assessed. The activation of PI3K-Akt signaling is measured by Western blot. Its effects on MMP-9 expression and activity are measured by zymography or ELISA. |
| Animal Protocol |
In vivo animal experiments for Sodium hyaluronate have been conducted in models of osteoarthritis, wound healing, and cancer. The compound is administered by injection or topical application, and its effects on joint function, wound closure, and tumor growth are assessed. Its use as a drug delivery vehicle has also been evaluated in various animal models.
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| ADME/Pharmacokinetics |
Sodium hyaluronate is a biopolymer with a molecular weight that can vary depending on the source and grade. It is a white to off-white powder that is highly soluble in water. It is typically stored at room temperature and protected from moisture. Its solutions are viscous and exhibit pseudoplastic behavior. The compound is stable under normal storage conditions.
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| Toxicity/Toxicokinetics |
Sodium hyaluronate is generally recognized as safe for medical use. It is biocompatible and biodegradable, with low immunogenicity. Adverse effects are rare and are typically limited to local reactions at the site of injection. It is well-tolerated in ophthalmic and orthopedic applications. Its safety has been established through extensive clinical use.
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| References | |
| Additional Infomation |
Sodium hyaluronate (hyaluronic acid sodium salt) is an endogenous glycosaminoglycan found in connective tissue and synovial fluid. It functions as a tissue lubricant and plays important roles in wound healing, signal transduction, and tumor physiology. It is widely used in medical applications, including ophthalmology, orthopedics, and dermatology, and is available from various suppliers for research and clinical purposes.
|
| Molecular Formula |
C28H42N2NA2O23
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|---|---|
| Molecular Weight |
1500
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| Exact Mass |
799.223
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| CAS # |
9067-32-7
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| Related CAS # |
Hyaluronic acid;9004-61-9
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| Appearance |
White to off-white solid powder
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| Density |
1.78g/cm3
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| Boiling Point |
791.6ºC
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| Flash Point |
432.5ºC
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| InChi Key |
PJIUBKBHEGKTJL-VUHXTTSISA-L
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| InChi Code |
InChI=1S/C28H44N2O23.2Na/c1-5(33)29-9-18(11(35)7(3-31)47-25(9)46)49-28-17(41)15(39)20(22(53-28)24(44)45)51-26-10(30-6(2)34)19(12(36)8(4-32)48-26)50-27-16(40)13(37)14(38)21(52-27)23(42)43/h7-22,25-28,31-32,35-41,46H,3-4H2,1-2H3,(H,29,33)(H,30,34)(H,42,43)(H,44,45)/q2*+1/p-2/t7-,8-,9-,10-,11-,12-,13+,14+,15-,16-,17-,18-,19-,20+,21+,22+,25-,26+,27-,28-/m1../s1
|
| Chemical Name |
sodium
(2S,3S,4R,5R,6R)-6-(((2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl)oxy)-3-(((2S,3R,4R,5S,6R)-3-acetamido-4-(((2R,3R,4S,5S,6S)-6-carboxylato-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)oxy)-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-4,5-dihydroxytetrahydro-2H-pyran-2-carboxylate
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| Synonyms |
Hyaluronic acid sodium Sodium hyaluronate Arthrease Bio Hyaluro 12 Hyalart Hyalurone sodium NRD101 NRD 101 NRD-101 SI-4402 SI4402 SI 4402
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~3.33 mg/mL DMSO : ~1 mg/mL)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 33.33 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6667 mL | 3.3333 mL | 6.6667 mL | |
| 5 mM | 0.1333 mL | 0.6667 mL | 1.3333 mL | |
| 10 mM | 0.0667 mL | 0.3333 mL | 0.6667 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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