HQNO

Cat No.:V2181 Purity: ≥98%
HQNO, secreted byP.
HQNO Chemical Structure CAS No.: 341-88-8
Product category: Mitochondrial Metabolism
This product is for research use only, not for human use. We do not sell to patients.
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

HQNO, secreted by P. aeruginosa, is a novel and potent electron transport chain inhibitor with a Kd of 64 nM for complex III. HQNO is a potent inhibitor of mitochondrial NDH-2 in many species.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
HQNO is effective in reducing free radicals that are highly reactive for mitochondrial type II NADH:quinone oxidase (NDH-2) in a variety of species, such as Staphylococcus aureus, Saccharomyces cerevisiae, Gluconobacter oxidans, Toxoplasma gondii, Yarrowia lipolytica, and Plasmodium falciparum. For wild-type (WT) and I379E C, the range of HQNO concentrations was 0 to 100 μM and 0 to 300 μM, respectively. thermarum NDH-2 variations, in accordance with IC50 values. WT NDH-2's IC50 value in the presence of 400μM methoquinone (MD) is 10.5±1.3μM HQNO. The IC50 value of HQNO reduced to 7.3±1.2 μM in the presence of 50 μM MD, while >50 μM HQNO resulted in near total suppression (~15% residual activity). HQNO inhibition was measured at 50μM MD, and the IC50 value was 54.3±1.2μM[2].
References
[1]. N J Jacobs, et al. Evidence for Involvement of the Electron Transport System at a Late Step of Anaerobic Microbial Heme Synthesis. Biochim Biophys Acta. 1977 Jan 6;459(1):141-4.
[2]. Jessica Petri, et al. Structure of the NDH-2 - HQNO Inhibited Complex Provides Molecular Insight Into Quinone-Binding Site Inhibitors. Biochim Biophys Acta Bioenerg. 2018 Jul;1859(7):482-490.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C16H21NO2
Molecular Weight
259.34344
CAS #
341-88-8
SMILES
CCCCCCCC1=[N+](C2=CC=CC=C2C(=C1)O)[O-]
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO : ~5 mg/mL (~19.28 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 3.8559 mL 19.2797 mL 38.5594 mL
5 mM 0.7712 mL 3.8559 mL 7.7119 mL
10 mM 0.3856 mL 1.9280 mL 3.8559 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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