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    Golgicide A (GCA)
    Golgicide A (GCA)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0188
    CAS #: 1139889-93-2Purity ≥98%

    Description: Golgicide A (GCA), a quinoline compound, is a cell-permeable, potent and rapidly reversible inhibitor of GBF1, which is one of the guanine nucleotide exchange factors (ArfGEFs). It is also an inhibitor of shiga toxin cytotoxicity. Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM in Vero cells. Immunofluoresence experiments demonstrates that Golgicide A causes complete dispersal of the medial-Golgi markers giantin and the cis-Golgi marker GM130 and results in a rapid redistribution of COPI from the Golgi.

    References: Nat Chem Biol. 2009 Mar;5(3):157-65; J Virol. 2011 Jan;85(2):946-56.

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    Molecular Weight (MW)284.3
    FormulaC17H14F2N2 
    CAS No.1139889-93-2
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 57 mg/mL (200.5 mM)
    Water:  <1 mg/mL 
    Ethanol: 4 mg/mL (14.1 mM)
    SMILESFC1=CC2=C(NC(C3=CC=CN=C3)[[email protected]@]4([H])[[email protected]]2([H])C=CC4)C(F)=C1
    SynonymsGolgicide A; GCA


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    In Vitro

    In vitro activity: Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM. Golgicide A causes a decrease in GBF1-mediated Arf1 activation, arrests secretion of soluble and membrane-anchored proteins and then impairs retrograde toxin transport. Golgicide A decreases HCV RNA levels in FLRP1 cells and J6/JFH1 cells. In addition, Golgicide A causes redistribution of NS5A and accumulation of infectious viral particles in J6/JFH1 cells.


    Cell Assay: Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM in Vero cells. Immunofluoresence experiments demonstrates that Golgicide A causes complete dispersal of the medial-Golgi markers giantin and the cis-Golgi marker GM130 and results in a rapid redistribution of COPI from the Golgi. Also, Golgicide A causes a decrease in GBF1-mediated Arf1 activation, impairs retrograde toxin transport and arrests secretion of soluble and membrane-anchored proteins. Golgicide A decreases HCV RNA levels and causes redistribution of NS5A in FLRP1 cells and J6/JFH1 cells. In addition, Golgicide A causes accumulation of infectious viral particles in J6/JFH1 cells.

    In VivoN/A
    Animal modelN/A
    Formulation & DosageN/A
    References

    Nat Chem Biol. 2009 Mar;5(3):157-65; J Virol. 2011 Jan;85(2):946-56.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    Golgicide A
    The effects of GCA are similar to expression of inactive GBF1-E794K. Nat Chem Biol. 2009 Mar;5(3):157-65.
     
    Golgicide A
    GCA causes a decrease in GBF1-dependent Arf1 activation. Nat Chem Biol. 2009 Mar;5(3):157-65.


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