| Size | Price | Stock | Qty |
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| 5g |
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| 10g |
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| 25g |
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| 50g |
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| Other Sizes |
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| ln Vitro |
Acyclic deoxyguanosine analog ganciclovir (BW 759) possesses strong anti-CMV action and resembles acyclovir in structure. Compared to 72 μM for acyclovir, the median dose of ganciclovir needed to suppress viral reproduction by 50% is 2.15 μM [4]. Using ganciclovir-5'-triphosphate (ganciclovir-TP), ganciclovir inhibits viral DNA replication, which is the primary mechanism of action against CMV. Viral DNA polymerase is specifically and potently inhibited by this inhibitor. Three biological enzymes mostly convert ganciclovir to its triphosphate form: phosphoglycerate kinase, guanylate kinase, and deoxyguanosine kinase, which is activated in CMV-infected cells [5].
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| ln Vivo |
For a total of five intraperitoneal injections, ganciclovir (BW 759) (50 mg/kg) drastically lowers white blood cells, red blood cells, and platelets in neonatal mice. It can also spread to the outer ear and brain. The space of lymph. 3]. In mice infected with the cytomegalovirus (MCMV), ganciclovir (1–80 mg/kg; intravenously; once daily for 5 days) postpones wasting syndrome and mortality [6].
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| Animal Protocol |
Animal/Disease Models: non-inbred Oncins France 1 (OF1) mice and albino rats without MCMV immunization [3]
Doses: 50 mg/kg Route of Administration: intraperitoneal (ip) injection, twice a day, for 5 consecutive injections (mouse ) or 3 days (adult rats) ) (pharmacokinetic/PK/PK study) Experimental Results: In adult rats, intracochlear diffusion of ganciclovir reaches the same concentration as in blood. In pregnant mice, transplacental spread was observed with a fetal to maternal blood ratio of 0.5. In neonatal mice, plasma concentrations of ganciclovir peak within 2 hrs (hrs (hours)) and gradually decrease thereafter. In adult mice, concentrations peaked at 1 hour but became undetectable at 2 hrs (hrs (hours)) post-injection. White blood cells, red blood cells and platelets were Dramatically diminished in newborn mice. Animal/Disease Models: Female SCID (severe combined immunodeficient) mouse vaccinated with MCMV[6] Doses: 0, 1, 10, 80 and 160 mg/kg Route of Administration: subcutaneous injection, one time/day for 5 days Experimental Results: Within a certain dose range, dose-dependent Delayed wasting syndrome |
| References |
[1]. Faulds D, et al. Ganciclovir. A review of its antiviral activity, pharmacokinetic properties and therapeutic efficacy in cytomegalovirus infections. Drugs. 1990;39(4):597-638.
[2]. Maggs DJ, et al. In vitro efficacy of ganciclovir, cidofovir, penciclovir, foscarnet, idoxuridine, and acyclovir against feline herpesvirus type-1. Am J Vet Res. 2004 Apr;65(4):399-403. [3]. Boujemla I, et al. Pharmacokinetics and tissue diffusion of ganciclovir in mice and rats. Antiviral Res. 2016;132:111-115. [4]. Fletcher CV, et al. Evaluation of ganciclovir for cytomegalovirus disease. DICP. 1989 Jan;23(1):5-12. [5]. Matthews T, et al. Antiviral activity and mechanism of action of ganciclovir. Rev Infect Dis. 1988 Jul-Aug;10 Suppl 3:S490-4. [6]. Duan J, Paris W, Kibler P, Bousquet C, Liuzzi M, Cordingley MG. Dose and duration-dependence of ganciclovir treatment against murine cytomegalovirus infection in severe combined immunodeficient mice. Antiviral Res. 1998;39(3):189-197. |
| Molecular Formula |
C9H12N5NAO4
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|---|---|
| Molecular Weight |
277.21
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| Elemental Analysis |
C, 38.99; H, 4.36
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| CAS # |
107910-75-8
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| Related CAS # |
Ganciclovir;82410-32-0;Ganciclovir hydrate;1359968-33-4
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| Appearance |
Solid powder
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| SMILES |
C1=NC2=C(N1COC(CO)CO)N=C(N=C2[O-])N.[Na+]
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| InChi Key |
JJICLMJFIKGAAU-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C9H13N5O4.Na/c10-9-12-7-6(8(17)13-9)11-3-14(7)4-18-5(1-15)2-16;/h3,5,15-16H,1-2,4H2,(H3,10,12,13,17);/q;+1/p-1
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| Chemical Name |
sodium;2-amino-9-(1,3-dihydroxypropan-2-yloxymethyl)purin-6-olate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 8 mg/mL(28.86 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (90.18 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6074 mL | 18.0369 mL | 36.0737 mL | |
| 5 mM | 0.7215 mL | 3.6074 mL | 7.2147 mL | |
| 10 mM | 0.3607 mL | 1.8037 mL | 3.6074 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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