Size | Price | Stock | Qty |
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100mg |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Fipronil is a phenylpyrazole-based insecticide with a broad-spectrum insecticidal activity. It acts by disrupting the insect CNS/central nervous system by blocking GABA-gated chloride channels and glutamate-gated chloride channels, leading to central nervous system toxicity. Fipronil can be used to kill parasites such as fleas and lice on cats and dogs.
ln Vitro |
High dosages of flupora mango (0.1–25 µM; 72 h) cause human hepatocytes to express CYP1A1 and CYP3A4 mRNA [1]. The induction of caspase-3/7 in HepG2 cells is time- and dose-dependent, with flupora mango (0.1-25 µM; 72 h) showing significant effects on caspase-3/7 activity, a key indicator of the cell sterilizing process [1].
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ln Vivo |
Fluorophora cockroach, with IC50 values of 800 and 10 nM, respectively, blocks both glutathione-induced chloride ion desensitizing and non-desensitizing currents [2]. With IC50 values of 28 and 35 nM in the calm and active states, respectively, fluorophora inhibits the intercepted GABA uptake [2].
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Cell Assay |
RT-PCR[1]
Cell Types: Human hepat cells Tested Concentrations: 0.1-25 µM Incubation Duration: 72 hrs (hours) Experimental Results: The expression of CYP1A1 and CYP3A4 mRNA was Dramatically increased in a dose-dependent manner. Apoptosis analysis[1] Cell Types: HepG2 Cell Tested Concentrations: 0.05, 0.1, 0.5, 1, 3.125, 6.25, 12.5, 25, 50 µM Incubation Duration: 24, 48, 72 hrs (hours) Experimental Results: Demonstrated time and dose dependence Induces caspase-3/7 activity from 0.1 µM to 6.25 µM, while slowly decreasing from 12.5 µM to 50 µM. |
References |
[1]. Das PC, et al. Fipronil induces CYP isoforms and cytotoxicity in human hepatocytes. Chem Biol Interact. 2006 Dec 15;164(3):200-14.
[2]. Narahashi T, et al. Glutamate-activated chloride channels: Unique fipronil targets present in insects but not in mammals. Pestic Biochem Physiol. 2010 Jun 1;97(2):149-152. |
Molecular Formula |
C12H4CL2F6N4OS
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Molecular Weight |
437.1414
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Exact Mass |
435.9387
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CAS # |
120068-37-3
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Related CAS # |
Fipronil-13C6
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SMILES |
ClC1C([H])=C(C(F)(F)F)C([H])=C(C=1N1C(=C(C(C#N)=N1)S(C(F)(F)F)=O)N([H])[H])Cl
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Synonyms |
MB-46030 HSDB-7051MB46030 HSDB7051MB 46030 HSDB 7051 Fipronil
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~250 mg/mL (~571.89 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.76 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.76 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.2876 mL | 11.4380 mL | 22.8760 mL | |
5 mM | 0.4575 mL | 2.2876 mL | 4.5752 mL | |
10 mM | 0.2288 mL | 1.1438 mL | 2.2876 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.