| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| 1g |
|
||
| 2g |
|
||
| 5g |
|
||
| Other Sizes |
|
Purity: ≥98%
Evodiamine (d-Evodiamine; Q-100579; SC-16015; Evo; Isoevodiamine) is a naturally occuring quinazolinocarboline alkaloid extracted from the fruit of Evodiae Fructus, exhibiting moderate antiproliferative activities against various human tumor cells. Evodiamine functions as a TRPV1 agonist that inhibits angiogenesis and inflammation. Through caspase-independent apoptotic pathways, it induces a significant amount of apoptosis in Bcl-2- and Akt-overexpressing U937 cells but not in human peripheral blood mononuclear cells. Evodiamine also prevents NF-kappaB and Akt activation brought on by tumor necrosis factor (TNF), but it has no impact on JNK or p38 MAPK activation.
| Targets |
NF-κB
|
|---|---|
| ln Vitro |
Evodiamine (Isoevodiamine), an alkaloid extract from Evodiae Fructus exhibits antitumor activities against the human tumor cells. Human peripheral blood mononuclear cells are not affected by evodiamine's substantial induction of apoptosis in Bcl-2- and Akt-overexpressing U937 cells, however. [1] NF-kappaB and Akt activation caused by tumor necrosis factor (TNF) is also inhibited by evodiamine, but it has no impact on JNK or p38 MAPK activation. [2] Evodiamine also functions as a human topoisomerase I inhibitor. [3]
|
| ln Vivo |
LD50: Mice 77mg/kg (i.v.) [4]
|
| Cell Assay |
Evodiamine is dissolved in DMSO and diluted before use with the proper medium. The MTT assay is used to test the new scaffolds for growth inhibitory activities against the human cancer cell lines A549 (lung cancer), MDA-MB-435 (breast cancer), and HCT116 (colon cancer). Camptithecin and evodiamine are used as examples.
|
| Animal Protocol |
Rats: Twelve male, healthy The control group, which received oral 10 mg/kg dapoxetine alone, and the combination group, which received oral 10 mg/kg dapoxetine co-administered with 100 mg/kg evodiamine, are both groups of Sprague-Dawley rats. Different pharmacokinetic parameters are calculated using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to estimate the plasma concentration of dapoxetine and desmethyl dapoxetine.
Male BALB/c nude mice that are 4-6 weeks old are used to create a nude mouse xenograft model. Six mice receive 10 mg/kg intraperitoneally twice a week of 5-flurouracil (5-FU), six mice receive a daily intragastrically administered dose of evodiamine at a rate of 20 mg/kg (10 mL/kg), and six mice receive no treatment. Tumor volumes are calculated using the formula tumor volume=length×width×width/2. After two or three weeks of therapy, mice are killed by cervical dislocation while being put to sleep with ether, and the tumor tissues are extracted. |
| References |
| Molecular Formula |
C19H17N3O
|
|
|---|---|---|
| Molecular Weight |
303.36
|
|
| Exact Mass |
303.14
|
|
| Elemental Analysis |
C, 75.23; H, 5.65; N, 13.85; O, 5.27
|
|
| CAS # |
518-17-2
|
|
| Related CAS # |
(±)-Evodiamine;518-18-3
|
|
| Appearance |
Solid powder
|
|
| SMILES |
CN1[C@@H]2C3=C(CCN2C(=O)C4=CC=CC=C41)C5=CC=CC=C5N3
|
|
| InChi Key |
TXDUTHBFYKGSAH-SFHVURJKSA-N
|
|
| InChi Code |
InChI=1S/C19H17N3O/c1-21-16-9-5-3-7-14(16)19(23)22-11-10-13-12-6-2-4-8-15(12)20-17(13)18(21)22/h2-9,18,20H,10-11H2,1H3/t18-/m0/s1
|
|
| Chemical Name |
(1S)-21-methyl-3,13,21-triazapentacyclo[11.8.0.02,10.04,9.015,20]henicosa-2(10),4,6,8,15,17,19-heptaen-14-one
|
|
| Synonyms |
|
|
| HS Tariff Code |
2934.99.9001
|
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
|
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
|
|||
|---|---|---|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (3.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2964 mL | 16.4821 mL | 32.9641 mL | |
| 5 mM | 0.6593 mL | 3.2964 mL | 6.5928 mL | |
| 10 mM | 0.3296 mL | 1.6482 mL | 3.2964 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA