Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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Other Sizes |
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ln Vitro |
The production of IL-6, IL-8, and MCP-1 is dose-dependently inhibited by a pretreatment of nereptin, with IC50 values of 4.43, 3.42, and 4.17 μM, respectively [1]. The expression of IL-1β-induced cytokine (IL-6, IL-8, and MCP-1) mRNA levels in ARPE-19 cells is inhibited by nereptin (2.5–10 μM; 25 hours) treatment [1]. Nepetin treatment (2.5-10 μM; 1.5 h; ARPE-19 cells) dose-dependently reduced p65's nuclear translocation and the phosphorylation of IKKα/β and IκBα. In activated ARPE-19 cells, napetin lowers the amounts of phosphorylated ERK1/2, JNK, and p38 MAPK [1].
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Cell Assay |
RT-PCR[1]
Cell Types: ARPE-19 Cell Tested Concentrations: 2.5 μM, 5 μM, 10 μM Incubation Duration: 25 hrs (hours) Experimental Results: Inhibition of the mRNA expression of IL-6, IL-8 and MCP-1 in ARPE-19 cells . Western Blot Analysis[1] Cell Types: ARPE-19 Cell Tested Concentrations: 2.5 μM, 5 μM, 10 μM Incubation Duration: 1.5 hrs (hours) Experimental Results: Dose-dependent inhibition of IKKα/β and IκBα phosphorylation and p65 nuclear translocation. Reduces the levels of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells. |
References |
[1]. Chen X, et al. Nepetin inhibits IL-1β induced inflammation via NF-κB and MAPKs signaling pathways in ARPE-19 cells. Biomed Pharmacother. 2018 May;101:87-93.
[2]. Militão GC, et al. Cytotoxic activity of nepetin, a flavonoid from Eupatorium ballotaefolium HBK. Pharmazie. 2004 Dec;59(12):965-6. |
Molecular Formula |
C16H12O7
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Molecular Weight |
316.265
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Exact Mass |
316.0583
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CAS # |
520-11-6
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SMILES |
O1C(=C([H])C(C2C(=C(C(=C([H])C1=2)O[H])OC([H])([H])[H])O[H])=O)C1C([H])=C([H])C(=C(C=1[H])O[H])O[H]
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~125 mg/mL (~395.24 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.58 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.1619 mL | 15.8093 mL | 31.6186 mL | |
5 mM | 0.6324 mL | 3.1619 mL | 6.3237 mL | |
10 mM | 0.3162 mL | 1.5809 mL | 3.1619 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.