| Size | Price | Stock | Qty |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
Epigoitrin exerts its effects through multiple mechanisms. It has antiviral activity against influenza A virus (FM1) by inhibiting virus attachment and multiplication. It reduces the host's susceptibility to influenza virus under stress. It also decreases norepinephrine formation by bovine dopamine-β-hydroxylase in a cell-free assay. Its antiviral activity is mediated through mitochondrial antiviral signaling.
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| ln Vitro |
In vitro, epigoitrin has antiviral activity against influenza A1 virus FM1 by inhibiting virus attachment and multiplication. It also decreases norepinephrine formation by bovine dopamine-β-hydroxylase. These activities have been characterized in various in vitro assays.
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| ln Vivo |
In vivo, epigoitrin provides protection against influenza infection by reducing the host's susceptibility to the influenza virus under stress. Its antiviral properties make it a candidate for research into novel antiviral treatments.
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| Enzyme Assay |
Non-cellular assays for epigoitrin involve assessing its inhibition of dopamine-β-hydroxylase using a cell-free system. The enzyme is incubated with its substrate, dopamine, and a cofactor in the presence of varying concentrations of epigoitrin. The formation of norepinephrine is measured, and the IC₅₀ for inhibition is determined. Its antiviral activity can be assessed in cell-free systems by measuring its direct interaction with viral proteins.
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| Cell Assay |
In vitro cellular assays for epigoitrin involve treating influenza virus-infected cells with the compound and measuring viral replication. Cells are infected with influenza A virus (FM1) and treated with varying concentrations of epigoitrin. Viral attachment and multiplication are assessed by measuring viral RNA levels (qRT-PCR) or viral titers (plaque assay). The EC₅₀ for inhibition of viral replication is determined.
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| Animal Protocol |
In vivo animal experiments with epigoitrin are conducted in mouse models of influenza infection. Mice are infected with influenza virus and treated with epigoitrin via oral administration. Viral load in the lungs is measured, and survival rates are assessed. The compound's ability to protect against influenza infection is evaluated.
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| ADME/Pharmacokinetics |
Epigoitrin has a molecular weight of 129.18 and a molecular formula of C₅H₇NOS. It is a yellow crystalline solid with a purity of ≥98%. The compound is soluble in DMSO and is typically stored at 2-8°C. It is stable under recommended storage conditions. Its IUPAC name is (5R)-5-ethenyl-1,3-oxazolidine-2-thione.
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| Toxicity/Toxicokinetics |
Epigoitrin is a research compound for laboratory use only and is not intended for human therapeutic or diagnostic use. In preclinical studies, it has been generally well-tolerated at therapeutic doses. Standard laboratory safety precautions should be followed when handling the compound.
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| References |
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| Additional Infomation |
(R)-Goitrin is a 5-vinyl-1,3-oxazolidine-2-thione with the R configuration. It is a component of the traditional Chinese medicine Isatis indigotica root. It possesses antiviral activity and is also a plant metabolite. It is the enantiomer of (S)-goitrin. Goitrin has been reported to be present in cabbage, Sophora flavescens, and other organisms with relevant data.
Epigoitrin ((R)-Goitrin; CAS 1072-93-1) is a natural alkaloid found in Isatis indigotica and cruciferous vegetables. It has antiviral activity against influenza A virus by inhibiting virus attachment and multiplication. Epigoitrin provides protection against influenza infection and is available from various commercial suppliers for research applications. |
| Molecular Formula |
C5H7NOS
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|---|---|
| Molecular Weight |
129.17
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| Exact Mass |
129.024
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| Elemental Analysis |
C, 46.49; H, 5.46; N, 10.84; O, 12.39; S, 24.82
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| CAS # |
1072-93-1
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| Related CAS # |
Goitrin;500-12-9
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| PubChem CID |
3032313
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| Appearance |
White to yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
150.6±43.0 °C at 760 mmHg
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| Melting Point |
50ºC
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| Flash Point |
44.9±28.2 °C
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| Vapour Pressure |
3.8±0.3 mmHg at 25°C
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| Index of Refraction |
1.570
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| LogP |
0.38
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
8
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| Complexity |
124
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| Defined Atom Stereocenter Count |
1
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| SMILES |
S=C1N([H])C([H])([H])[C@@]([H])(C([H])=C([H])[H])O1
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| InChi Key |
UZQVYLOFLQICCT-SCSAIBSYSA-N
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| InChi Code |
InChI=1S/C5H7NOS/c1-2-4-3-6-5(8)7-4/h2,4H,1,3H2,(H,6,8)/t4-/m1/s1
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| Chemical Name |
2-Oxazolidinethione, 5-vinyl-, (R)-
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| Synonyms |
Epigoitrin; (R)-Goitrin; (R) Goitrin; BA-51-090278; BA 51-090278; BA 51 090278; BA 51090278; BA51090278
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~387.06 mM)
Ethanol : ~3.33 mg/mL (~25.78 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (19.35 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (19.35 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (19.35 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 10% DMSO+40% PEG300+5% Tween-80+45% Saline: ≥ 2.5 mg/mL (19.35 mM) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.7417 mL | 38.7087 mL | 77.4174 mL | |
| 5 mM | 1.5483 mL | 7.7417 mL | 15.4835 mL | |
| 10 mM | 0.7742 mL | 3.8709 mL | 7.7417 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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