Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Purity: ≥98%
dTRIM24 is a novel, potent and selective PROTAC degrader of TRIM24 with anticancer activity. The addressable pocket of a protein is often not functionally relevant in disease. This is true for the multidomain, bromodomain-containing transcriptional regulator TRIM24. TRIM24 has been posited as a dependency in numerous cancers, yet potent and selective ligands for the TRIM24 bromodomain do not exert effective anti-proliferative responses. Recruitment of the VHL E3 ubiquitin ligase by dTRIM24 elicits potent and selective degradation of TRIM24.
ln Vitro |
The TRIM24 bromodomain is degraded by dTRIM24. Efficient and targeted degradation of TRIM24 is initiated by the recruitment of VHL E3 ubiquitin ligase by dTRIM24. evaluating the effects of TRIM24 inhibition and chemical degradation on antiproliferative outcomes. dTRIM24, IACS-9571, VL-269, and eTRIM24-treated MOLM-13 cells were grown and their growth was tracked throughout time. Compared to IACS-9571, dTRIM24 significantly reduced growth, and this was followed by increased PARP cleavage, which is a sign of apoptosis. Throughout the trial, nearly total TRIM24 degradation was seen in dTRIM24-treated cells, which is consistent with the prolonged proliferation defect shown following dTRIM24 treatment [2].
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References |
[1]. Gechijian LN, et al. Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands. Nat Chem Biol. 2018 Apr;14(4):405-412
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Molecular Formula |
C55H68N8O13S2
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Molecular Weight |
1113.30423164368
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CAS # |
2170695-14-2
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SMILES |
O=C([C@H]1N(C([C@H](C(C)(C)C)NC(COCCOCCOCCNC(C2=CC=CC(S(=O)(NC3=C(OC4=CC=CC(OCCC)=C4)C=C(C(N5C)=C3)N(C)C5=O)=O)=C2)=O)=O)=O)C[C@H](O)C1)NCC6=CC=C(C7=C(C)N=CS7)C=C6
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InChi Key |
QUQTXFIMYFMULC-OGOZKGDESA-N
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InChi Code |
InChI=1S/C55H68N8O13S2/c1-8-20-75-40-12-10-13-41(28-40)76-47-30-45-44(61(6)54(69)62(45)7)29-43(47)60-78(70,71)42-14-9-11-38(26-42)51(66)56-19-21-72-22-23-73-24-25-74-33-48(65)59-50(55(3,4)5)53(68)63-32-39(64)27-46(63)52(67)57-31-36-15-17-37(18-16-36)49-35(2)58-34-77-49/h9-18,26,28-30,34,39,46,50,60,64H,8,19-25,27,31-33H2,1-7H3,(H,56,66)(H,57,67)(H,59,65)/t39-,46+,50-/m1/s1
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Chemical Name |
(2S,4R)-1-((S)-15-(tert-Butyl)-1-(3-(N-(1,3-dimethyl-2-oxo-6-(3-propoxyphenoxy)-2,3-dihydro-1H-benzo[d]imidazol-5-yl)sulfamoyl)phenyl)-1,13-dioxo-5,8,11-trioxa-2,14-diazahexadecan-16-oyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide
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Synonyms |
dTRIM-24; dTRIM 24; dTRIM24
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
MEthanol : ~150 mg/mL (~134.73 mM)
DMSO : ~100 mg/mL (~89.82 mM) Ethanol :< 1 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.8982 mL | 4.4912 mL | 8.9823 mL | |
5 mM | 0.1796 mL | 0.8982 mL | 1.7965 mL | |
10 mM | 0.0898 mL | 0.4491 mL | 0.8982 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Design and chemical characterization of dTRIM24 as a degrader of TRIM24. Global displacement of TRIM24 from chromatin and transcriptional response to TRIM24 degradation. From: Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.Nat Chem Biol.2018 Apr;14(4):405-412. th> |
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Characterization of the cellular mechanism of degradation of dTRIM24. Design and chemical characterization of dTRIM24 as a degrader of TRIM24. From: Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.Nat Chem Biol.2018 Apr;14(4):405-412. td> |
Genetic dependency on TRIM24 in acute leukemia. Antiproliferative effect of selective TRIM24 degradation in acute leukemia. From: Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHLNat Chem Biol.2018 Apr;14(4):405-412. td> |