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    Docetaxel (RP 56976)
    Docetaxel (RP 56976)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1604
    CAS #: 114977-28-5 Purity ≥98%

    Description: Docetaxel, also known as RP56976 and NSC 628503, an analog of paclitaxel, is a mitotic inhibitor that inhibits the depolymerization of microtubules by binding to and stabilizing microtubules. Docetaxel has shown potent and a broad spectrum of antineoplastic activities that has been extensively used in the treatment of various cancers. It is a semi-synthetic, second-generation taxane derived from a compound found in the European yew tree Taxus baccata. Docetaxel displays potent and broad antineoplastic properties; it binds to and stabilizes tubulin, thereby inhibiting microtubule disassembly which results in cell- cycle arrest at the G2/M phase and cell death.

    References: Biochem Biophys Res Commun. 1992 Aug 31;187(1):164-70; J Immunol. 1999 Jan 1;162(1):467-73.

    Related CAS#: 148408-66-6 (hydrate); 167074-97-7 [10-Oxo Docetaxel, Docetaxel Impurity]; 162784-72-7 [7-Epi-10-oxo-docetaxel, Docetaxel Impurity]; 153381-68-1 [7-Epi-docetaxel, 4-epi-Docetaxel; 7-Epidocetaxel; 7-Epitaxotere]

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    Molecular Weight (MW)807.88
    FormulaC43H53NO14 
    CAS No.114977-28-5 (Docetaxel); 
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 100 mg/mL (123.8 mM)
    Water:<1 mg/mL
    Ethanol: 53 mg/mL (65.6 mM)
    Solubility (In vivo)5% DMSO+30% PEG 300+5% Tween 80+ddH2O: 2 mg/mL 
    Synonyms

    RP56976; RP-56976; RP 56976; NSC 628503; NSC628503; NSC-628503; Docetaxel hydrate, Trade name: Taxotere.

    Chemical Name: (2aR,4S,4aS,6R,9S,11S,12S,12bS)-12b-acetoxy-9-(((2R,3S)-3-((tert-butoxycarbonyl)amino)-2-hydroxy-3-phenylpropanoyl)oxy)-4,6,11-trihydroxy-4a,8,13,13-tetramethyl-5-oxo-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-1H-7,11-methanocyclodeca[3,4]benzo[1,2-b]oxet-12-yl benzoate. 

    SMILES Code: O=C(O[[email protected]@H](C1[[email protected]@]2(C)[[email protected]@H](O)C[[email protected]@]3([H])OC[[email protected]]31OC(C)=O)[[email protected]]4(O)C[[email protected]](OC([[email protected]](O)[[email protected]@H](NC(OC(C)(C)C)=O)C5=CC=CC=C5)=O)C(C)=C(C4(C)C)[[email protected]@H](O)C2=O)C6=CC=CC=C6


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    In Vitro

    In vitro activity: Docetaxel is a cytotoxic agent, especially for proliferating cells, which is related to its ability to promote the formation of microtubule bundles and induce sustained mitotic arrest, followed by apoptosis of mitotically arrested cells or permanent mitotic block. Docetaxel suppresses microtubule dynamic instability as well as tread-milling, resulting in the failure of chromosomes to segregate to the daughter cells, which in turn triggers premature exit from mitosis rather than a block at this phase of the cell cycle. Docetaxel inhibits the clonogenic survival of Human cancer cell Hs746T (stomach), AGS (stomach), HeLa (cervix), CaSki (cervix), BxPC3 (pancreas), Capan-1 (pancreas) with IC50 of 1 nM, 1 nM, 0.3 nM, 0.3 nM, 0.3 nM and 0.3 nM respectively. Docetaxel inhibits endothelial cell migration that does not affects microtubule gross morphology or inhibit cell proliferation, although they does produce more subtle effects on microtubule dynamics. Docetaxel inhibits HUVEC migration with an observed IC50 of 1 pM. HUVEC chemotaxis stimulated by either of two angiogenic factors, thymidine phosphorylase or VEGF, is inhibited by Docetaxel with IC 50 of 10 pM and is ablated at 1 NM.


    Kinase Assay: Single-drug concentration-response curves are assessed. Seeding is done at a density of 2,000 cells/well for PC-3 and LNCaP, in 96-well plates. Cells are treated with each single drug and their combination for 72 h at different drug concentrations. Docetaxel is used at concentrations of 0.1-1,000 nM. GLU is used at concentrations of 0.1-300 µm. Cytotoxicity is assessed at the end of drug exposure using SRB assay. Following 72 h exposure the cells are fixed with 10% trichloroacetic acid (150 µL) for 1 h at 4°C. Then, cells are stained for 10 min at room temperature with 0.4% SRB dissolved in 1% acetic acid. The plates are then air dried for 24 h and the dye is made soluble with 150 µL Tris (10 mM, PH 7.4) for 5 min on a shaker at 1,600 rpm. Absorbance is then measured at 545 nM using microplate reader. Results are expressed as the relative percentage of absorbance compared to control.


    Cell Assay: 2000 cells in 180 μL of medium are seeded in a 96-well plate, and 20 μL of drug solution is simultaneously added in triplicate to each well. The plate is incubated for 3 days at 37°C in a humidified atmosphere of 5% CO2. On day 3, 25 μL of MTT reagent is added to each well. After 4 h of incubation, the medium is removed by aspiration. 0.2 mL of dimethylsulphoxide (DMSO) is added to each well and thoroughly mixed by using a mechanical plate mixer to dissolve the resulting MTT-formazan crystals. Absorbance at 540 nm (OD) is measured by a reader.

    In VivoDocetaxel (33 mg/kg/dose, given i.v. every 4 days for 3 injections) results in a tumor growth delay of 19.3 days in M2OL2 colon xenografts. Docetaxel also shows great antitumor activities in MX-1, SK-MEL-2, LX-1 and OVCAR-3 xenografts. Docetaxel inhibits the angiogenic response to fibroblast growth factor 2 with IC 50 of 5.4 mg/kg when injected twice weekly over a 14-day period, and angiogenesis is completely blocked in mice that receives 10 mg/kg Docetaxel. Docetaxel has selectivity for endothelial cell migration and/or microvessel formation because infiltration of inflammatory cells into the Matrigel plug is much less sensitive to inhibition by Docetaxel.
    Animal modelHuman colon carcinomas xenografts CX-1
    Formulation & DosageDissolved in 50 mg/mL stock solution in ethanol by adding an equal volume of polysorbate 80 and diluting with 5% dextrose in water to the final volume;  33 mg/kg; i.v. injection 
    ReferencesBiochem Biophys Res Commun. 1992 Aug 31;187(1):164-70; J Immunol. 1999 Jan 1;162(1):467-73.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    Docetaxel

     

    Docetaxel

     


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