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    DMOG (dimethyloxaloylglycine)
    DMOG (dimethyloxaloylglycine)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0936
    CAS #: 89464-63-1Purity ≥98%

    Description: DMOG, formerly known as dimethyloxaloylglycine, is a novel, potent and cell permeable antagonist of α-ketoglutarate cofactor and competitive inhibitor for HIF prolyl hydroxylase. It acts to stabilize HIF-1α expression at normal oxygen tensions in cultured cells, at concentrations between 0.1 and 1 mM. DMOG ia able to  provide neuroprotection after traumatic brain injury. DMOG can provide enhanced efficacy with reduced systemic exposure in a murine model of colitis. DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC

    References: Biochem J. 1994 Jun 1;300 ( Pt 2):525-30; J Physiol. 2004 Oct 1;560(Pt 1):21-6.

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    Molecular Weight (MW)175.14
    FormulaC6H9NO5
    CAS No.89464-63-1
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 35 mg/mL (199.8 mM)
    Water: 35 mg/mL (199.8 mM)
    Ethanol: 35 mg/mL (199.8 mM)
    SMILES Code O=C(OC)CNC(C(OC)=O)=O
    SynonymsDimethyloxalylglycine


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    In Vitro

    In vitro activity: DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC.


    Kinase Assay:


    Cell Assay: To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

    In VivoDMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats.  
    Animal modelC57Bl6 Mice
    Formulation & Dosage8 mg DMOG dissolved in 0.5 ml saline; 8 mg/mouse; i.p. injection
    References

    Biochem J. 1994 Jun 1;300 ( Pt 2):525-30; J Physiol. 2004 Oct 1;560(Pt 1):21-6; Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    DMOG

    Immunohistochemistry of HIF-1α protein expression in mouse skeletal muscle. J Physiol.2004 Oct 1;560(Pt 1):21-6.
     

    DMOG

    Effect of DMOG treatment on protein expression by Western analysis in ischaemic and non-ischaemic mouse EDL . J Physiol. 2004 Oct 1;560(Pt 1):21-6.
     

    DMOG

    Biochem J. 1994 Jun 1;300 ( Pt 2):525-30.


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