| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
Darbufelone (Ki=10±5 μM) inhibits PGHS-2 in a non-competitive manner. At 325 nm (lambda(ex)=280 nm), darbufelone quenches the fluorescence of PGHS-2, with a Kd of 0.98±0.03 μM[1]. A549, H520, and H460 cell lines—which were developed based on three distinct histological subtypes of non-small cell lung cancer (NSCLC)—were utilized to assess the potential antiproliferative effects of darbufelone (adenocarcinoma, squamous cell carcinoma, and large cell lung cancer, respectively). Elevate the concentration of dabufilon (between 5 and 60 μM) and conduct a 72-hour test. With increasing drug concentrations, these three cell lines' cytostatic effects grew over time. A549 and H520 had IC50 values of 20±3.6 and 21±1.8 μM, respectively, but H460 has a substantially lower IC50 value of 15±2.7 μM [2].
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| ln Vivo |
Darbufelone is a dual inhibitor of LTB4 and PGF2R synthesis in cells. In animal models of inflammation and arthritis, dabufilon is both orally active and non-ulcerogenic [1]. Tumor volume decreased in mice receiving dabufilone at a dose of 80 mg/kg/day in a time-dependent manner. Conversely, dabufilone at lesser dosages (20 or 40 mg/kg/day) did not significantly reduce the weight of tumors. Mice given dabufilon (80 mg/kg/day) at necropsy showed a 30.2% decrease in tumor weight as compared to controls [2].
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| References |
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| Molecular Formula |
C18H24N2O2S.CH4O3S
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|---|---|
| Molecular Weight |
428.56602
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| Exact Mass |
428.144
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| CAS # |
139340-56-0
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| Related CAS # |
Darbufelone;139226-28-1
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| PubChem CID |
6439420
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| Appearance |
White to light yellow solid powder
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| Boiling Point |
448.6ºC at 760mmHg
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| Flash Point |
225.1ºC
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| Vapour Pressure |
1.16E-08mmHg at 25°C
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| LogP |
5.139
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
28
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| Complexity |
609
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(C)(C)C1=CC(=CC(=C1O)C(C)(C)C)/C=C\2/C(=O)N=C(S2)N.CS(=O)(=O)O
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| InChi Key |
BAZGFSKJAVQJJI-CHHCPSLASA-N
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| InChi Code |
InChI=1S/C18H24N2O2S.CH4O3S/c1-17(2,3)11-7-10(8-12(14(11)21)18(4,5)6)9-13-15(22)20-16(19)23-13;1-5(2,3)4/h7-9,21H,1-6H3,(H2,19,20,22);1H3,(H,2,3,4)/b13-9-;
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| Chemical Name |
(5Z)-2-amino-5-[(3,5-ditert-butyl-4-hydroxyphenyl)methylidene]-1,3-thiazol-4-one;methanesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~110 mg/mL (~256.67 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 5.5 mg/mL (12.83 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 55.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3333 mL | 11.6667 mL | 23.3334 mL | |
| 5 mM | 0.4667 mL | 2.3333 mL | 4.6667 mL | |
| 10 mM | 0.2333 mL | 1.1667 mL | 2.3333 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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