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| 25mg |
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Cyclosporin D is a nonpolar cyclic oligopeptide and a novel metabolite of Cyclosporin A, which is an immunosuppressant agent that binds cyclophilin D, inhibiting the phosphatase activity of calcineurin in T cells. Cyclosporin D is a minor analogue of the cyclosporin complex produced by Trichoderma.
| ln Vitro |
In vitro, EF-2 phosphorylation that is dependent on Ca2+/calmodulin is inhibited by cyclosporin D [1].
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| ln Vivo |
In vivo biological effects of phorbol ester TPA on mouse skin are strongly inhibited by cyclosporine D [1].
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| References |
[1]. Gschwendt M, et al. The weak immunosuppressant cyclosporine D as well as the immunologically inactive cyclosporine H are potent inhibitors in vivo of phorbol ester TPA-induced biological effects in mouse skin and of Ca2+/calmodulin dependent EF-2 phosphorylation in vitro. Biochem Biophys Res Commun. 1988 Jan 29;150(2):545-51.
[2]. Kaiser P, et al. A new approach for the determination of immunosuppressive drugs using HPLC-MS/MS and Cs+ adducts. Ger Med Sci. 2006 Jan 18;4:Doc01. [3]. Minguillón J, et al. Concentrations of cyclosporin A and FK506 that inhibit IL-2 induction in human T cells do not affect TGF-beta1 biosynthesis, whereas higher doses of cyclosporin A trigger apoptosis and release of preformed TGF-beta1. J Leukoc Biol. 2005 May;77(5):748-58. Epub 2005 Feb 16. |
| Molecular Formula |
C₆₃H₁₁₃N₁₁O₁₂
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|---|---|
| Molecular Weight |
1216.64
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| CAS # |
63775-96-2
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| SMILES |
C/C=C/C[C@H]([C@@H](O)[C@H]1C(N[C@H](C(N(CC(N([C@H](C(N[C@H](C(N([C@H](C(N[C@H](C(N[C@@H](C(N([C@H](C(N([C@H](C(N([C@H](C(N1C)=O)C(C)C)C)=O)CC(C)C)C)=O)CC(C)C)C)=O)C)=O)C)=O)CC(C)C)C)=O)C(C)C)=O)CC(C)C)C)=O)C)=O)C(C)C)=O)C
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| Synonyms |
Cyclosporin DVal2-cyclosporine
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~20.55 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.05 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8219 mL | 4.1097 mL | 8.2194 mL | |
| 5 mM | 0.1644 mL | 0.8219 mL | 1.6439 mL | |
| 10 mM | 0.0822 mL | 0.4110 mL | 0.8219 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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