Ryanodine receptors (RyRs) – no IC50/Ki/EC50 values provided in this reference. [1]

Cyantraniliprole (also known as HGW-86), an anthranilic diamide analog, is a novel and potent second-generation insecticide which is able to deter insect feeding and reduce disease transmission. Cyantraniliprol is a valuable tool for managing Frankliniella occidentalis (Pergande) and also is as effective as abamectin in deterring potato psyllid feeding and could significantly reduce transmission of Lso and the spread of ZC. Cyantraniliprole is approved for use in the United States, Canada, China, and India. Because of its uncommon mechanism of action as a ryanoid, it has activity against pests such as Diaphorina citri that have developed resistance to other classes insecticides. Cyantraniliprole is highly toxic to bees, which resulted in registration of its use as a pesticide being delayed in the USA.

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Kinase Assay:

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Cell Assay:

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Cyantraniliprole contact toxicity (glass vial method, 24 h) against Diaphorina citri adults: LC50 = 0.27 μg AI/mL (95% CI: 0.18–0.43). [1]
Cyantraniliprole contact toxicity against Tamarixia radiata (primary parasitoid) adults: LC50 = 80.23 μg AI/mL (95% CI: 11.20–149.00); the LC50 for T. radiata was 297‑fold higher than for D. citri. [1]
Sublethal effects on D. citri feeding: honeydew excretion was significantly reduced at all tested concentrations (0.13–10.00 μg AI/mL) compared to control (Fig. 1). [1]
Sublethal effects on settling behavior: at 72 h after release, significantly fewer D. citri adults settled on citrus plants treated with 0.025 μg AI/mL or higher concentrations compared to control (Fig. 2). [1]
Sublethal effects on developmental stages: egg production significantly reduced at ≥0.25 μg AI/mL; first‑instar emergence significantly reduced at ≥0.02 μg AI/mL; adult emergence significantly reduced at ≥0.25 μg AI/mL (Fig. 3). [1]

Foliar application of cyantraniliprole (HGW 10SE, 1.5 L/ha, 1436 g AI/ha) significantly reduced D. citri nymph ranking (0.35 ± 0.04) and adults per tap (0.30 ± 0.05) compared to fenpropathrin (0.96 and 0.99) and untreated control (1.59 and 1.32) over 8‑9 weeks post‑treatment (Tables 2, 3). [1]
Foliar application also significantly reduced citrus leafminer (Phyllocnistis citrella) larvae (1.16 ± 0.17 per flush) and pupae (0.03 ± 0.01) compared to fenpropathrin and control (Table 3). [1]
Drench application of cyantraniliprole (HGW 20SC) at 2.2 L/ha significantly reduced D. citri nymph ranking (0.44 ± 0.05) and adults per tap (0.09 ± 0.02) compared to thiamethoxam and control (Table 4). [1]
Drench application also significantly reduced P. citrella larvae and pupae at all tested rates (1.1, 1.5, 2.2 L/ha) compared to thiamethoxam and control (Table 5). [1]

Glass vial toxicity bioassay: 20 mL glass scintillation vials were coated with 1 mL of technical‑grade cyantraniliprole dissolved in acetone or acetone alone, rotated for 30 min to evaporate acetone. 20–30 adult D. citri or T. radiata were placed in each vial (6 concentrations, 5–6 replicates). Vials were held at 25±2 °C, 50±5% RH, 14:10 h light:dark for 24 h. Mortality was assessed and corrected with Abbott’s formula; LC50 and 95% CI calculated by probit regression. [1]
Feeding assay (honeydew excretion): citrus leaf discs (60 mm) were dipped in cyantraniliprole (0.13–10.00 μg AI/mL) or water, air‑dried 1 h, placed in agar‑coated petri dishes. Five D. citri adults were added, dishes sealed with filter paper, turned upside down, incubated for 48 h. Filter papers were stained with ninhydrin; honeydew droplets counted. [1]
Settling behavior assay: citrus plants (14–16 weeks old) were sprayed with cyantraniliprole (0.02–5.00 μg AI/mL) or water until runoff. Six plants (5 treated + control) were placed in a plexiglass cage (choice test). 50 D. citri adults released per cage; number settled on each plant recorded at 24, 48, 72 h. Six cages (replicates). [1]
Developmental stages assay: potted citrus plants (2–3 months old) were sprayed with cyantraniliprole (0.02–5.00 μg AI/mL) or water, air‑dried, then exposed to 5 pairs of D. citri adults for 72 h (covered). After removal, eggs per plant counted. First‑instar nymphs counted at 3 days; adult emergence monitored weekly. Experiment repeated twice. [1]
Field foliar application: 5‑year‑old ‘Navel’ citrus trees, randomized complete block design with 4 replicates (4 trees per replicate). Treatments: cyantraniliprole (HGW 10SE, 1.5 L/ha), fenpropathrin (1.2 L/ha), untreated control. Foliar spray with hand gun at 200 psi, 0.3 gal/tree. Sampling for D. citri nymphs (10 feather flush/replicate, ranking 0‑3) and adults (10 stem taps/replicate) weekly for 8‑9 weeks, starting 3 days post‑application. P. citrella larvae and pupae counted per flush. [1]
Field drench application: similar design with 5 treatments: cyantraniliprole (HGW 20SC) at 1.1, 1.5, 2.2 L/ha, thiamethoxam (0.8 L/ha), untreated control. Drench applied with vehicle‑mounted system at 0.26 L/tree within drip line. Sampling as above. [1]

Pest Manag Sci.2013 Sep;69(9):1066-72.

Cyanobacterium is a carboxamide insecticide, a derivative of chlorantraniliprole in which the chlorine atom on the benzene ring is replaced by a cyano group. It is a rannidazole receptor agonist used as an insecticide to control whiteflies, thrips, aphids, fruit flies, and fruit borers on crops such as onions, potatoes, and tomatoes. It is highly toxic to bees. It is a pyrazole insecticide, a nitrile compound, an organochlorine compound, an organobromine compound, a pyridine compound, and a secondary carboxamide.

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Cyantraniliprole (Cyazypyr™) is a second‑generation anthranilic diamide. It binds to insect ryanodine receptors, causing uncontrolled Ca²⁺ release from sarcoplasmic reticulum, leading to muscle paralysis and death. It has root systemic and translaminar activity. In this study, it demonstrated high efficacy against Asian citrus psyllid (Diaphorina citri) and citrus leafminer (Phyllocnistis citrella) with significant sublethal effects (reduced feeding, settling, oviposition, egg hatch, adult emergence). It was 297‑fold less toxic to the parasitoid Tamarixia radiata than to D. citri, suggesting compatibility with biological control. Foliar and drench applications provided long‑lasting residual control (>1 month). This chemistry is recommended for rotation with neonicotinoids in resistance management programs. [1]

C19H14BRCLN6O2

473.71

472.005

736994-63-1

Cyantraniliprole-d3;1392493-34-3

11578610

White to off-white solid powder

1.612g/cm3

561.256ºC at 760 mmHg

293.237ºC

1.704

4.433

2

5

4

29

672

0

O=C(C1=CC(Br)=NN1C2=NC=CC=C2Cl)NC3=C(C(NC)=O)C=C(C#N)C=C3C

DVBUIBGJRQBEDP-UHFFFAOYSA-N

InChI=1S/C19H14BrClN6O2/c1-10-6-11(9-22)7-12(18(28)23-2)16(10)25-19(29)14-8-15(20)26-27(14)17-13(21)4-3-5-24-17/h3-8H,1-2H3,(H,23,28)(H,25,29)

5-bromo-2-(3-chloropyridin-2-yl)-N-[4-cyano-2-methyl-6-(methylcarbamoyl)phenyl]pyrazole-3-carboxamide

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.

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Solubility in Formulation 2: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.

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 (Please use freshly prepared in vivo formulations for optimal results.)