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CTB (also known as Cholera Toxin B subunit) is a potent activator of p300 histone acetyltransferase and induces apoptosis in MCF-7 cells.
| ln Vitro |
CTB increases p300 HAT activity in a dose-dependent manner (10, 50, 100, 150, 200, and 250 μM; 10 min) [1]. MCF-7 cells' viability is inhibited by CTB (0 - 200 μM; 24 hours). There is a time-dependent induction of MCF-7 cells by CTB (85.43 μM; 24, 48, and 72 hours) [2]. With an IC50 of 85.43 μM, CTB (50 μM; 24 h) boosts p300/CBP activity[2].
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|---|---|
| Cell Assay |
Cell viability assay [2]
Cell Types: MCF-7 Cell Tested Concentrations: 0-200 μM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibits viability with IC50 of 85.43 μM and reduces autophagic corrosion in primary neurons [3]. . Apoptosis analysis [2] Cell Types: MCF-7 Cell Tested Concentrations: 85.43 μM Incubation Duration: 24, 48 and 72 h Experimental Results: Time-dependent apoptosis was induced. |
| References |
[1]. Mantelingu K, et al. Activation of p300 histone acetyltransferase by small molecules altering enzyme structure: probed by surface-enhanced Raman spectroscopy. J Phys Chem B. 2007;111(17):4527-4534.
[2]. Dastjerdi MN, et al. The effect of CTB on P53 protein acetylation and consequence apoptosis on MCF-7 and MRC-5 cell lines. Adv Biomed Res. 2013;2:24. Published 2013 Mar 6. [3]. Chen X, et al. Promoting tau secretion and propagation by hyperactive p300/CBP via autophagy-lysosomal pathway in tauopathy. Mol Neurodegener. 2020;15(1):2. Published 2020 Jan 6. |
| Molecular Formula |
C16H13NO2F3CL
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|---|---|
| Molecular Weight |
343.728
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| CAS # |
451491-47-7
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| SMILES |
ClC1=CC=C(NC(=O)C2=CC=CC=C2OCC)C=C1C(F)(F)F
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~290.93 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.27 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.27 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9093 mL | 14.5463 mL | 29.0926 mL | |
| 5 mM | 0.5819 mL | 2.9093 mL | 5.8185 mL | |
| 10 mM | 0.2909 mL | 1.4546 mL | 2.9093 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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