Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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25mg |
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50mg |
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Other Sizes |
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Purity: ≥98%
Corydaline is a bioactive and naturally occurring isoquinoline alkaloid extracted from Corydalis yanhusuo. It exhibits anti-angiogenic, anti-allergic and gastric-emptying and antinociceptive activities by acting as an AChE inhibitor.
ln Vitro |
Corydaline treatment (12.5-50 μM; 24 hours) reduces COX-2 production and activation of JNK MAPK and P38 MAPK, but not ERK MAPK, which prevents EV71 replication [2].
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ln Vivo |
Corydanine (10 mg/kg; subcutaneous; once) therapy revealed analgesic effects by dramatically decreasing writhing behavior in mice [3].
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Cell Assay |
Western Blot analysis [2]
Cell Types: Vero cells infected with EV71 Tested Concentrations: 12.5μM, 25μM, 50μM Incubation Duration: 24 hrs (hours) Experimental Results: The phosphorylation and expression of P38MAPK and JNKMAPK were diminished. COX-2. |
Animal Protocol |
Animal/Disease Models: Male CD1 mice (30-35 g, 7-8 weeks old) were injected with acetic acid [3].
Doses: 10 mg/kg. Route of Administration: subcutaneousadministration; primary Experimental Results:demonstrated anti-nociceptive effect on mice. |
References |
[1]. Hai-Tao Xiao, et al. Acetylcholinesterase inhibitors from Corydalis yanhusuo. Nat Prod Res. 2011 Sep;25(15):1418-22.
[2]. Hui-Qiang Wang, et al. Corydaline inhibits enterovirus 71 replication by regulating COX-2 expression. J Asian Nat Prod Res. 2017 Nov;19(11):1124-1133. [3]. Teresa Kaserer, et al. Identification and characterization of plant-derived alkaloids, corydine and corydaline, as novel mu opioid receptor agonists. Sci Rep. 2020 Aug 14;10(1):13804. |
Molecular Formula |
C22H27NO4
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Molecular Weight |
369.4541
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CAS # |
518-69-4
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SMILES |
O(C([H])([H])[H])C1=C(C([H])=C([H])C2=C1C([H])([H])N1C([H])([H])C([H])([H])C3=C([H])C(=C(C([H])=C3[C@@]1([H])[C@@]2([H])C([H])([H])[H])OC([H])([H])[H])OC([H])([H])[H])OC([H])([H])[H]
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~90.22 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.77 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.7067 mL | 13.5336 mL | 27.0673 mL | |
5 mM | 0.5413 mL | 2.7067 mL | 5.4135 mL | |
10 mM | 0.2707 mL | 1.3534 mL | 2.7067 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.