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    Fosbretabulin disodium (CA 4DP)
    Fosbretabulin disodium (CA 4DP)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1617
    CAS #: 168555-66-6 Purity ≥98%

    Description: Combretastatin A4 Phosphate Disodium (also called CA4P, Fosbretabulin; CA 4DP; CA-4P; Zybrestat) is the water-soluble prodrug that has to be activated to the active form, Combretastatin A4 (CA4), which is a highly potent MTA (microtubule-targeting agent) or tubulin inhibitor that acts as a potent anticancer agent that binds β-tubulin with a Kd of 0.4 μM in a cell-free assay. It was originally isolated from African tree Combretum caffrum. Combretastatin A4 inhibits the polymerization of tubulin with an IC50 of 2.4 μM, and also disrupts tumor vasculature. Under in vivo conditions, the prodrug fosbretabulin is dephosphorylated and activated to its active form, combretastatin A4, which binds to tubulin dimers and prevents microtubule polymerization, resulting in mitotic arrest and apoptosis in endothelial cells. 

    References: Br J Cancer. 1995 Apr;71(4):705-11; Cancer Res. 1997 May 15;57(10):1829-34.

    Related CAS#: 222030-63-9 (free acid); 168555-66-6 (disodium); 404886-32-4 ( tromethamine)

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    Molecular Weight (MW)440.29 
    FormulaC18H19O8P.2Na 
    CAS No.168555-66-6 (disodium); 
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: <1 mg/mL
    Water: 28 mg/mL (63.59 mM)
    Ethanol: <1 mg/mL
    Solubility (In vivo)Saline with a few drops of 5% Na2CO3: 30 mg/mL 
    SynonymsCA 4DP; CA 4P; Combretastatin A4 disodium phosphate; Combretastatin A4 phosphate disodium; CA4P; CA4DP; Combretastatin A4 phosphate; Fosbretabulin disodium; Zybrestat


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    In Vitro

    In vitro activity: Fosbretabulin disodium (Combretastatin A-4 phosphate disodium, CA4P disodium) is the water-soluble prodrug of combretastatin A4 (CA4), which is originally isolated from African tree Combretum caffrum. CA4 is a tubulin-binding agent that binds at or near the colchicine binding site of β-tubulin (Kd = 0.40 μM), inhibits tubulin assembly with IC50 of 2.4 μM. CA4 is cytotoxic towards proliferating but not quiescent endothelial cells, has potent and selective toxicity towards tumor vasculature. CA4P (1 mM, 30 minutes) disrupts the endothelial microtubule cytoskeleton and mediates changes in endothelial cell morphology. CA4P stimulates actin stress fiber formation and membrane blebbing and increases monolayer permeability via Rho/Rho-kinase. CA4P increases endothelial cell permeability, while inhibiting endothelial cell migration and capillary tube formation predominantly through disruption of VE-cadherin/β-catenin/Akt signaling pathway, thereby leading to rapid vascular collapse and tumor necrosis.


    Kinase Assay: The assembly of microtubules from isolated tubulin is carried out spectrophotometrically at 350 nm and utilises the increase in turbidity which is associated with microtubule formation. Assembly is initiated by temperature increase from 10 to 35 °C. The effect of drugs on the increase in light absorption is carried. Drugs are dissolved in DMSO (<4%), which does not affect control assembly.


    Cell Assay: For the proliferation assay, the minimal concentration of FBS (1%) diluted in X-VIVO medium is used to allow sufficient viability of endothelial cells. After detachment, the cells are seeded at a concentration of 2×104 HUVECs in each well of 24-well plates, allowed to adhere overnight, and then incubated with or without cytokines (5 ng/ml FGF-2 or 5 ng/ml VEGF-A). CA4P is added at 0 – 50 nM. After incubation for 12, 24, 36, and 48 hours, cells are detached by trypsin/EDTA and manually counted using trypan blue exclusion.

    In VivoCA4P causes rapid, extensive and irreversible vascular shutdown in experimental tumor models following the administration of a single dose at 10% of the maximum tolerated dose (MTD). CA4P causes a 93% reduction in vascular volume 6 h following drug administration. CA4P(100 mg/kg, 6 h following administration) reduces tumor blood by approximately 100-fold, compared with approximately 7-fold in the spleen. 
    Animal modelBD9 rats implanted with tumor 
    Formulation & DosageDissolved in 0.9% saline with a few drops of 5% Na2CO3; 100 mg/kg, 3 ml/kg; i.p. injection
    References

    Br J Cancer. 1995 Apr;71(4):705-11; Cancer Res. 1997 May 15;57(10):1829-34. 


    These protocols are for reference only. InvivoChem does not independently validate these methods.

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