Cidofovir (GS 0504)

Alias: HPMPC; Cidofovir; GS-504; GS 504;Vistide; HPMPC; (S)-HPMPC; GS-504
Cat No.:V1444 Purity: ≥98%
Cidofovir (also known as HPMPC; GS-0504; HPMPC; (S)-HPMPC; Vistide) is a potent and injectable antiviral medication approved for the treatment of cytomegalovirus (CMV) retinitis with IC50of 0.94 μM.
Cidofovir (GS 0504) Chemical Structure CAS No.: 113852-37-2
Product category: DNA(RNA) Synthesis
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
10mg
25mg
50mg
100mg
250mg
500mg
1g
Other Sizes

Other Forms of Cidofovir (GS 0504):

  • Cidofovir hydrate (HPMPC)
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Cidofovir (also known as HPMPC; GS-0504; HPMPC; (S)-HPMPC; Vistide) is a potent and injectable antiviral medication approved for the treatment of cytomegalovirus (CMV) retinitis with IC50 of 0.94 μM. It is frequently used to treat AIDS patients' cytomegalovirus (CMV) retinitis. In cultured cells, cidofovir prevents human cytomegalovirus (HCMV) infection. Even when added to the cells 48 hours after infection, cidofovir inhibits the formation of CMV plaques, with an IC50 of 0.9 μg/mL for the Davis strain and 1.6 μg/mL for the AD-169 strain, respectively.

Biological Activity I Assay Protocols (From Reference)
ln Vitro

In vitro activity: Cidofovir inhibits the growth of the human cytomegalovirus (HCMV) in cultured cells. With an IC50 of 0.9 μg/mL for the Davis and 1.6 μg/mL for the AD-169 strains, respectively, cidofovir inhibits the formation of CMV plaques in cells even when added 48 hours after infection. [1] Cidofovir also prevents the infection of the herpes simplex virus. Cidofovir also inhibits the production of viral DNA and the expression of proteins specific to HSV-l in monkey kidney cells, as well as cell fusion brought on by HSV-1.[3]

ln Vivo
Cidofovir (5 mg/kg/day) administered subcutaneously for five days dramatically lowers the average virus infectivity titer in the salivary gland, spleen, lung, and blood of infected guinea pigs. Infected animals with cidofovir have significantly lower splenic average tissue index and lymphocytosis.[2]. All signs (skin lesions, hindlimb paralysis, and death) in hairless mice intracutaneously infected with HSV-1 or HSV-2 are suppressed by cidofovir. The most noteworthy aspect of cidofovir is that it provides strong protection against HSV-1 or HSV-2 infection with just one dose—even up to four days after infection. Cidofovir suppresses the growth of the extremely aggressive melanoma tumor that develops from subcutaneously grafted mouse melanoma B16 cells in C57B16/J mice.[5]
Cell Assay
Cell Line: Crandell-Reese feline kidney(CRFK) cells
Concentration: 10-100 μM
Incubation Time: 72 hours
Result: Reduced CRFK cells by 9.1%.
Animal Protocol
Female weanling BALB/c mice infected with cowpox virus (CPV)
100 mg/kg
Subcutaneous injection; 3-6 days interval; 21 days
References

[1]. Antimicrob Agents Chemother . 1988 Dec;32(12):1839-44.

[2]. Antiviral Res . 1990 May;13(5):237-52.

[3]. Antiviral Res . 1992 Sep;19(3):181-92.

[4]. Antimicrob Agents Chemother . 1991 Apr;35(4):701-6.

[5]. Br J Dermatol . 2000 Oct;143(4):741-8.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C8H14N3O6P
Molecular Weight
279.19
Exact Mass
279.06
Elemental Analysis
C, 34.42; H, 5.05; N, 15.05; O, 34.38; P, 11.09
CAS #
113852-37-2
Appearance
Solid powder
SMILES
C1=CN(C(=O)N=C1N)C[C@@H](CO)OCP(=O)(O)O
InChi Key
VWFCHDSQECPREK-LURJTMIESA-N
InChi Code
InChI=1S/C8H14N3O6P/c9-7-1-2-11(8(13)10-7)3-6(4-12)17-5-18(14,15)16/h1-2,6,12H,3-5H2,(H2,9,10,13)(H2,14,15,16)/t6-/m0/s1
Chemical Name
[(2S)-1-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxypropan-2-yl]oxymethylphosphonic acid
Synonyms
HPMPC; Cidofovir; GS-504; GS 504;Vistide; HPMPC; (S)-HPMPC; GS-504
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: <1 mg/mL
Water: <1 mg/mL
Ethanol: <1 mg/mL
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 3.5818 mL 17.9090 mL 35.8179 mL
5 mM 0.7164 mL 3.5818 mL 7.1636 mL
10 mM 0.3582 mL 1.7909 mL 3.5818 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
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  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
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  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Clinical Trial Information
NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01295645 Active
Recruiting
Drug: Cidofovir
Other: No Cidofovir
Transplantation Infection M.D. Anderson Cancer Center March 17, 2011 Phase 2
NCT00000799 Completed Drug: Cidofovir
Drug: Probenecid
Cytomegalovirus Retinitis
HIV Infections
National Institute of Allergy
and Infectious Diseases
(NIAID)
April 1996 Not Applicable
NCT00550589 Completed Drug: cidofovir
Procedure: biopsy
Precancerous Condition
Anal Cancer
AIDS Malignancy Consortium September 2007 Phase 2
NCT00001126 Completed Drug: Cidofovir Papilloma National Institute of Allergy
and Infectious Diseases
(NIAID)
November 2005 Phase 1
NCT02931539 Completed Drug: Maribavir
Drug: Ganciclovir
Cytomegalovirus (CMV) Shire December 22, 2016 Phase 3
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