| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| Other Sizes |
|
| ln Vitro |
At 70 μg/mL and an IC50 of 1.4 μM, (+)-catechin demonstrates >95% inhibitory action against cyclooxygenase-1 (COX-1) [1]. Following a 24-hour treatment with (+)-catechin, a dose-dependent decrease in color was noted. At the highest concentration of (+)-catechin tested (160 μg/mL), 54.76% of cells perished, with an IC50 of (+)-catechin at 127.62 μg/mL. Treatment of MCF-7 cells with (+)-catechin resulted in an increase in the induction of apoptosis that was dose- and time-dependent. After 24 hours, 40.7% and 41.16% of the cells treated with 150 μg/mL and 300 μg/mL (+)-catechin experienced apoptosis, in comparison to the control cells. Following a 24-hour treatment with 150 μg/mL (+)-catechin, MCF-7 cells exhibited 5.81, 1.42, 3.29, and 2.68-fold increases in Caspase-3, -8, and -9 expression levels, respectively. Comparison with untreated control cell levels [2].
|
|---|---|
| ln Vivo |
Although the difference was not statistically significant, animals treated with (+)-catechin at the lowest tested dose (i.e., 50 mg/kg, po) explored unfamiliar targets substantially more frequently in choice trials. Time-induced episodic memory losses are prevented by (+)-catechin in a dose-dependent manner; 200 mg/kg orally is the most efficacious dose. Treatment with (+)-catechin prevented an increase in MPO levels (21.98±9.44% in the hippocampus and 36.76±4.39% in the frontal cortex) compared to the DOX treatment group alone [3].
|
| References |
[1]. Waffo-Téguo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. Nutr Cancer. 2001;40(2):173-9.
[2]. Alshatwi AA. Catechin hydrate suppresses MCF-7 proliferation through TP53/Caspase-mediated apoptosis. J Exp Clin Cancer Res. 2010 Dec 17;29:167. [3]. Cheruku SP, et al. Catechin ameliorates doxorubicin-induced neuronal cytotoxicity in in vitro and episodic memory deficit in in vivo in Wistar rats. Cytotechnology. 2018 Feb;70(1):245-259 |
| Molecular Formula |
C15H14O6
|
|---|---|
| Molecular Weight |
290.2681
|
| CAS # |
225937-10-0
|
| Related CAS # |
(±)-Catechin;7295-85-4;Catechin;154-23-4
|
| SMILES |
O1C2=C([H])C(=C([H])C(=C2C([H])([H])[C@@]([H])([C@@]1([H])C1C([H])=C([H])C(=C(C=1[H])O[H])O[H])O[H])O[H])O[H]
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4451 mL | 17.2253 mL | 34.4507 mL | |
| 5 mM | 0.6890 mL | 3.4451 mL | 6.8901 mL | |
| 10 mM | 0.3445 mL | 1.7225 mL | 3.4451 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
