Size | Price | Stock | Qty |
---|---|---|---|
5mg |
|
||
10mg |
|
||
25mg |
|
||
50mg |
|
||
100mg |
|
||
250mg |
|
||
500mg |
|
||
Other Sizes |
|
Purity: ≥98%
Cardiogenol C is a diaminopyrimidine compound that induces the differentiation of MHC- (myosin heavy chain) positive cardiomyocytes from embryonic stem cells with an EC50 of 0.1 µM. Cardiogenol C upregulates cardiac markers and induces cardiac functional properties in lineage-committed progenitor cells. Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
ln Vitro |
When compared to untreated control cells, P19 cells treated with 1 μM of cardiogenol C hydrochloride for 7 days exhibit cardiogenic effects and considerably higher levels of atrial natriuretic factor (ANF, nppa) [1]. Heartogenol C hydrochloride (0.01–100 μM; 7 days) markedly elevated the expression of ANF. Additionally, this little chemical dramatically enhanced the expression of another widely used cardiac marker gene (NKX2-5) in C2C12 cells [2]. In C2C12 cells, cardiac Nav1.5 sodium channel protein expression is upregulated in a dose-dependent manner by cardiogenol C hydrochloride (0.001-100 μM; 7 days) [2]. The addition of 1 μM of cardiogenol C hydrochloride for 35 days, beginning on day 0, resulted in a considerable increase in the CB percentage of beating cardiomyocytes and in myocardial differentiation. This tiny chemical encourages the heart's developing beating cardiomyocytes, which are produced from cardiovascular progenitor cells [2]. Even at 10 μM, cardiogenol C hydrochloride (0.01-100 μM; 7 days) has no effect on cell proliferation. Moreover, Cardiogenol C dissolved in DMSO or water can have comparable results. On C2C12 cells, the maximum dosage of 100 μM exhibits notable cytotoxicity [2].
|
---|---|
Cell Assay |
RT-PCR[2]
Cell Types: C2C12 Cell Tested Concentrations: 0.01 μM; 0.1μM; 1μM; 3μM; 10μM; 30μM; 100 μM Incubation Duration: 7 days Experimental Results: ANF and NKX2.5 mRNA levels increased in a dose-dependent manner. Western Blot Analysis[2] Cell Types: C2C12 Cell Tested Concentrations: 0.001 μM; 0.01μM; 0.1μM; 1μM; 10 μM Incubation Duration: 7 days Experimental Results: Increased cardiac Nav1.5 sodium channel protein levels. Cell proliferation experiment [2] Cell Types: C2C12 cell Tested Concentrations: 0.01 μM; 0.1 μM; 1 μM Incubation Duration: 7 days Experimental Results: 0.01-10 μM treatment had no toxic effect on C2C12 cells. |
References |
[1]. Wu X, et al. Small molecules that induce cardiomyogenesis in embryonic stem cells.J Am Chem Soc. 2004 Feb 18;126(6):1590-1.
[2]. Mike AK, et al. Small molecule cardiogenol C upregulates cardiac markers and induces cardiac functional properties in lineage-committed progenitor cells. Cell Physiol Biochem. 2014;33(1):205-21. |
Molecular Formula |
C13H17CLN4O2
|
---|---|
Molecular Weight |
296.755
|
CAS # |
1049741-55-0
|
Related CAS # |
Cardiogenol C;671225-39-1
|
SMILES |
InChI=1S/C13H16N4O2.ClH/c1-19-11-4-2-10(3-5-11)16-13-15-7-6-12(17-13)14-8-9-18/h2-7,18H,8-9H2,1H3,(H2,14,15,16,17)1H
|
InChi Key |
2-((2-((4-methoxyphenyl)amino)pyrimidin-4-yl)amino)ethan-1-ol hydrochloride
|
InChi Code |
QQAHYSZVJLHCNA-UHFFFAOYSA-N
|
Chemical Name |
Cardiogenol C Cardiogenol C HCl Cardiogenol C hydrochloride.
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
DMSO : ≥ 59 mg/mL (~198.82 mM)
H2O : ~2 mg/mL (~6.74 mM) |
---|---|
Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (8.42 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.42 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 3.33 mg/mL (11.22 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.3697 mL | 16.8486 mL | 33.6973 mL | |
5 mM | 0.6739 mL | 3.3697 mL | 6.7395 mL | |
10 mM | 0.3370 mL | 1.6849 mL | 3.3697 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.