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    Carboplatin
    Carboplatin

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1425
    CAS #: 41575-94-4Purity ≥98%

    Description: Carboplatin (formerly known as JM-8, CBDCA, NSC-241240; Paraplat; Paraplatin; Blastocarb; Carboplat; Carbosin; Carbosol; Carbotec; Displata; Ercar) is an approved anticancer drug that acts as a DNA synthesis inhibitor by binding to DNA (DNA alkylator) and interfering with the cell's repair mechanism in cancer cells. It is used to treat some forms of cancer (mainly ovarian carcinoma, lung, head and neck cancers). It is activated intracellularly to form reactive platinum complexes that bind to nucleophilic groups such as GC-rich sites in DNA, thereby inducing intrastrand and interstrand DNA cross-links, as well as DNA-protein cross-links. These carboplatin-induced DNA and protein effects result in apoptosis and cell growth inhibition. 

    References: Cancer Chemother Pharmacol. 2008 Oct;62(5):769-78; Mol Cancer Ther. 2012 Sep;11(9):1948-58.

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    Molecular Weight (MW)371.25
    FormulaC6H12N2O4Pt
    CAS No.41575-94-4
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO:<1 mg/mL
    Water: 14 mg/mL (37.7 mM)
    Ethanol:<1 mg/mL
    Solubility (In vivo)Water: 14 mg/mL 
    SynonymsJM-8; NSC-241240; JM8; NSC241240; JM 8; NSC 241240; CBDCA; Carboplatin Hexal; Carboplatino; US trade names: Paraplat; Paraplatin; Foreign brand names: Blastocarb; Carboplat; Carbosin; Carbosol; Carbotec; Displata; Ercar; Nealorin; Novoplatinum; Paraplatin AQ; Paraplatine; Platinwas; Ribocarbo


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    In Vitro

    In vitro activity: Carboplatin exhibits an inhibitory effect on cell proliferation in a human ovarian cancer cell line panel, including A2780, SKOV3, and IGROV-1 cells with IC50 of 6.1 μM, 12.4 μM and 2.2 μM, respectively. Carboplatin also show the anti-proliferative activities in lung carcinoid cell line, such as UMC-11, H727, and H835 cells with IC50 of 36.4 μM, 3.4 μM and 35.8 μM, respectively.


    Cell Assay: 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assays: Exponentially growing A2780, SKOV3, IGROV-1 and HX62 ovarian cancer cells are plated in 96 well plates. A range of drug concentrations are added and the plates are incubated for 72 hours to allow for 3–4 doubling times. Each experiment is carried out in triplicate. Sulforhodamine B (SRB) assays: Exponentially growing A2780 cells are plated in 96 well microtitre plates. For experiments studying concomitant exposure, cells are exposed to increasing concentrations of both drugs for 96 hours. For experiments studying the effect of sequence of exposure to 17-AAG or carboplatin cells are exposed to increasing concentrations of 17-AAG or carboplatin for 24 hours. A period of 24-hour exposure to the first agent is chosen so that the A2780 cells would be exposed to the first drug for at least one doubling time (18-24 hours). The cells are then washed with sterile phosphate buffered saline and the medium is replenished. Following this, the second drug (to which the cells are not exposed to in the first 24 hours) or medium is added for 96 hours. All experiments are carried out in triplicate. The results of combination studies are analyzed using the well-established principles of median effect analysis method. The effects of the combination are calculated using an in-house spreadsheet.

    In VivoIn A2780 tumor xenografts, Carboplatin (60 mg/kg via i.p.) given as single agents shows a modest antitumor effect with the relative tumor volumes on day 6 of 8.4 compared to the control of 11.9, and the day 6 tumor weights relative to control (T/C) of 67%. For the VC8 (Brca2-deficient) xenografts, Carboplatin treatment delays tumor growth and reduces tumor mass by 42% compared to the vehicle group.
    Animal modelFmale athymic NCr nude mice (nu/nu) with  A2780 human ovarian cancer xenograft
    Formulation & DosageDissolved in 43% ethanol, 33% polypropylene glycol and 24% cremaphor diluted 1:7 with sterile water; 60 mg/kg; i.p. injection
    References

    Cancer Chemother Pharmacol. 2008 Oct;62(5):769-78; Mol Cancer Ther. 2012 Sep;11(9):1948-58.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

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