Size | Price | Stock | Qty |
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50mg |
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100mg |
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250mg |
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500mg |
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Other Sizes |
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Purity: ≥98%
Capsaicin ((E)-Capsaicin), a mixture of Capsaicin and Dihydrocapsaicin, is an active component isolatedd from chili peppers, which are plants belonging to the genus Capsicum, acting as a TRPV1 agonist with an EC50 of 0.29 μM in HEK293 cells. It is an irritant for mammals, including humans, and produces a sensation of burning in any tissue with which it comes into contact. Pure capsaicin is a volatile, hydrophobic, colorless, odorless, crystalline to waxy compound.
ln Vitro |
In a dose- and time-dependent way, capsaicin (50–300 µM; 24-72 hours) significantly inhibited cell proliferation. It is estimated that the IC50 value is 150 µM[2]. Over a 24-72-hour period, capsaicin (50-300 µM) increases the expression of pro-apoptotic Bad/Bax and decreases anti-apoptotic Bcl-2 protein. It also activates caspase 3 and PARP (p85) levels in the cytosol[2]. Sub-G1 DNA concentration, nuclear condensation, and nuclear DNA fragmentation are all increased by capsaicin [2]. By downregulating the production of cyclin B1 and D1 regulatory factors as well as cyclin-dependent protein kinases cdk-1, cdk-2, and cdk-4, capsaicin prevents cell cycle progression in the G1/S phase in FaDu cells [2].
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ln Vivo |
By altering the protein expression of the apoptotic regulatory factors p53, Bcl-2, Bax, and caspase-3, capsaicin prevents the growth of lung cancer [2].
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Cell Assay |
Cell Viability Assay[2]
Cell Types: Human Pharyngeal Squamous Carcinoma Cells (FaDu) Tested Concentrations: 50 µM, 100 µM, 200 µM and 300 µM Incubation Duration: 24 hrs (hours), 48 hrs (hours) and 72 hrs (hours) Experimental Results: Cell growth shown. Apoptosis analysis[2] Cell Types: FaDu Cell Tested Concentrations: 50 µM, 100 µM and 200 µM Incubation Duration: 12 hrs (hours) Experimental Results: The activity of caspase 3 increased in a time-dependent manner. Western Blot Analysis[2] Cell Types: FaDu Cell Tested Concentrations: 200 µM Incubation Duration: 24 hrs (hours) Experimental Results: Activation of caspase 3 and PARP (p85) levels was observed. |
Animal Protocol |
Animal/Disease Models: Benzo(a)pyrene-induced Swiss albino mice (20-25 g; 8-10 weeks old) [3]
Doses: 10 mg/kg Route of Administration: intraperitonealadministration; intraperitonealadministration. Once a week for 14 consecutive weeks Experimental Results: Inhibits the development of lung cancer in mice. |
References |
[1]. McNamara FN, et al. Effects of piperine, the pungent component of black pepper, at the human vanilloid receptor (TRPV1). Br J Pharmacol. 2005 Mar;144(6):781-90.
[2]. Shin YH, et al. The Effect of Capsaicin on Salivary Gland Dysfunction. Molecules. 2016 Jun 25;21(7). [3]. Anandakumar P, et al. Capsaicin provokes apoptosis and restricts benzo(a)pyrene induced lung tumorigenesis in Swiss albino mice. Int Immunopharmacol. 2013 Jun 6;17(2):254-259. |
Molecular Formula |
C18H27NO3
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Molecular Weight |
293.4012
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CAS # |
404-86-4
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Related CAS # |
Capsaicinoid;404-86-4;(E/Z)-Capsaicin-d3;1185237-43-7;(Z)-Capsaicin;25775-90-0;Capsaicin-d3;1217899-52-9
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SMILES |
CC(C)/C=C/CCCCC(NCC1=CC=C(O)C(OC)=C1)=O
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InChi Key |
YKPUWZUDDOIDPM-SOFGYWHQSA-N
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InChi Code |
InChI=1S/C18H27NO3/c1-14(2)8-6-4-5-7-9-18(21)19-13-15-10-11-16(20)17(12-15)22-3/h6,8,10-12,14,20H,4-5,7,9,13H2,1-3H3,(H,19,21)/b8-6+
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Chemical Name |
8-Methyl-N-vanillyl-(trans)-6-nonenamide
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Synonyms |
(E)-Capsaicin Capsicine Capsicin PS C (E)Capsaicin
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~327.43 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 20 mg/mL (65.49 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 200.0 mg/mL clear EtOH stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.19 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.19 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: ≥ 2.5 mg/mL (8.19 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL corn oil and mix evenly. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.4083 mL | 17.0416 mL | 34.0832 mL | |
5 mM | 0.6817 mL | 3.4083 mL | 6.8166 mL | |
10 mM | 0.3408 mL | 1.7042 mL | 3.4083 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.