Buspirone HCl (MJ90221)

Alias: Buspirone; MJ 9022 1; Buspar; MJ-9022-1; MJ90221; MJ9022 1; MJ9022-1
Cat No.:V0971 Purity: ≥98%
Buspirone HCl (MJ90221,MJ-90221,Buspar,MJ-9022-1, MJ9022 1, MJ9022-1), the hydrochloride salt of Buspirone which is anazapirone anolog,is a potent serotonergic 5HT1A receptor agonist with anti-psychotic effects.
Buspirone HCl (MJ90221) Chemical Structure CAS No.: 33386-08-2
Product category: 5-HT Receptor
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
100mg
250mg
500mg
1g
2g
5g
10g
Other Sizes

Other Forms of Buspirone HCl (MJ90221):

  • Buspirone free base
  • Buspirone-d8 hydrochloride (buspirone d8 hydrochloride)
Official Supplier of:
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Buspirone HCl (MJ90221, MJ-90221, Buspar, MJ-9022-1, MJ9022 1, MJ9022-1), the hydrochloride salt of Buspirone which is an azapirone anolog, is a potent serotonergic 5HT1A receptor agonist with anti-psychotic effects. Buspirone is a psychotropic anxiolytic that has been approved for use. Generalized anxiety disorder (GAD) is the main condition it is used to treat. Buspirone's pharmacology is unrelated to that of benzodiazepines or barbiturates, unlike most medications used primarily to treat anxiety, so there is no chance of physical dependence or withdrawal symptoms.

Biological Activity I Assay Protocols (From Reference)
Targets
5-HT1A
ln Vitro

In vitro activity: Buspirone is a non-benzodiazepine anxiolytic medication that is clinically effective. When administered intravenously (ED50 = 0.011 mg/kg, i.v.), intraperitoneally (ED50 = 0.088 mg/kg, i.p.), or intragastrically (effective dose = 1.0-20.0 mg/kg, i.g.), it inhibits the firing of these neurons. When administered externally via microiontophoresis to recorded neurons, buspirone also suppresses these cells.[1] Buspirone is mostly eliminated by oxidative metabolism, which results in the production of 1-pyrimidinylpiperazine and 5-hydroxy-buspirone, two hydroxylated metabolites.[2]

ln Vivo
Buspirone (3 mg/rat, i.p.) inhibits shock-induced ultrasonic vocalization both after systemic injection and after microinjection into the rat's dorsal raphe nucleus, which is home to a large number of somatodendritic 5-HT1A receptors. The inhibition is dose dependent and total. In the rat forced swim test, immobility is reduced by buspirone (20 mg/kg).[4] In a zebrafish model of anxiety, buspirone is a serotonergic (5HT(1A) receptor agonist) anxiolytic medication with some D(2) dopaminergic effect.[5] Buspirone acts at the 5-HT(1A) receptor in l-DOPA-primed rats, which reduces LID and improves l-DOPA-related motor performance in a dose-dependent manner. Buspirone improves the anti-parkinsonian efficacy of l-DOPA while delaying the development of LID, suggesting the potential long-term benefits of 5-HT(1A) agonists for lowering l-DOPA-related side effects in rats that have never taken the drug.[6]
Cell Assay
Cell Line: Lymphocytes
Concentration: 0, 4, 20, 40, 200 and 400 µg/mL
Incubation Time: 6 hours
Result: Decreased cell viability in a dose-dependent manner.
Animal Protocol
Male C57BL/6N mice
1 and 5 mg/kg
Oral gavage and intraperitoneal injection; for 5 days
References

[1]. Eur J Pharmacol . 1986 Sep 23;129(1-2):123-30.

[2]. Am J Med . 1986 Mar 31;80(3B):41-51.

[3]. Eur J Pharmacol . 1993 Nov 16;249(3):341-51.

[4]. Pharmacol Biochem Behav . 2009 Nov;94(1):75-80.

[5]. Pharmacol Biochem Behav . 2007 Aug-Sep;87(3):306-14.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C21H32CLN5O2
Molecular Weight
421.96
Exact Mass
421.22
Elemental Analysis
C, 59.77; H, 7.64; Cl, 8.40; N, 16.60; O, 7.58
CAS #
33386-08-2
Appearance
White crystalline powder
SMILES
C1CCC2(C1)CC(=O)N(C(=O)C2)CCCCN3CCN(CC3)C4=NC=CC=N4.Cl
InChi Key
RICLFGYGYQXUFH-UHFFFAOYSA-N
InChi Code
InChI=1S/C21H31N5O2.ClH/c27-18-16-21(6-1-2-7-21)17-19(28)26(18)11-4-3-10-24-12-14-25(15-13-24)20-22-8-5-9-23-20;/h5,8-9H,1-4,6-7,10-17H2;1H
Chemical Name
8-[4-(4-pyrimidin-2-ylpiperazin-1-yl)butyl]-8-azaspiro[4.5]decane-7,9-dione;hydrochloride
Synonyms
Buspirone; MJ 9022 1; Buspar; MJ-9022-1; MJ90221; MJ9022 1; MJ9022-1
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 50~84 mg/mL (118.5~199.1 mM)
Water: ~84 mg/mL (~199.1 mM)
Ethanol: ~36 mg/mL (~85.3 mM)
Solubility (In Vivo)
15% Captisol, pH 9: 10 mg/mL
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.3699 mL 11.8495 mL 23.6989 mL
5 mM 0.4740 mL 2.3699 mL 4.7398 mL
10 mM 0.2370 mL 1.1849 mL 2.3699 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
/

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
+
+
+

Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Clinical Trial Information
NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01850355 Active
Recruiting
Drug: Buspirone Anxiety
Autism Spectrum Disorders
Massachusetts General Hospital July 2013 Not Applicable
NCT02313194 Active
Recruiting
Device: Epidural Stimulation
Drug: Buspirone
Tetraparesis
Tetraplegia
Cervical Spinal Cord Injury
University of California,
Los Angeles
July 2013 Phase 1
Phase 2
NCT05041322 Recruiting Drug: Buspirone
Drug: Placebo
Spinal Cord Injuries Spaulding Rehabilitation Hospital November 29, 2020 Phase 2
NCT01821690 Recruiting Drug: Buspirone
Drug: Placebo
Traumatic Brain Injury Indiana University May 2013 Not Applicable
NCT05511909 Recruiting Drug: Buspirone
Drug: Lofexidine
Drug: Placebo
Opioid Use Disorder
Opioid Withdrawal
Opioid Craving
Anxiety
Johns Hopkins University December 15, 2022 Phase 2
Biological Data
  • Buspirone effects on bottom dwelling (sec/min) in zebrafish (mean ± sem). Pharmacol Biochem Behav . 2009 Nov;94(1):75-80.
  • Buspirone effects on swimming activity (log cm/min) in zebrafish (mean ± sem). Pharmacol Biochem Behav . 2009 Nov;94(1):75-80.
Contact Us