Bromfenac Sodium (AHR 10282R)

Alias: AHR 10282R; Bromfenac sodium, Prolensa, AHR-10282R;AHR10282R;Bromday, Xibrom, AHR-10282, AHR10282, AHR 10282
Cat No.:V1048 Purity: ≥98%
Bromfenac Sodium (formerly AHR-10282R;AHR10282R;AHR-10282, AHR10282; trade names Prolensa,Bromday, Xibrom), an approved nonsteroidal anti-inflammatory drug (NSAID), is an orally bioavailable COX-1/2 inhibitor with anti-inflammatory activity.
Bromfenac Sodium (AHR 10282R) Chemical Structure CAS No.: 91714-93-1
Product category: COX
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
100mg
250mg
500mg
1g
2g
5g
10g
Other Sizes

Other Forms of Bromfenac Sodium (AHR 10282R):

  • Bromfenac Sodium Sesquihydrate
  • Bromfenac
  • Bromfenac-d4 sodium (bromfenac sodium-d4)
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Bromfenac Sodium (formerly AHR-10282R; AHR10282R; AHR-10282, AHR10282; trade names Prolensa, Bromday, Xibrom), an approved nonsteroidal anti-inflammatory drug (NSAID), is an orally bioavailable COX-1/2 inhibitor with anti-inflammatory activity. It can inhibit the biosynthesis of prostaglandin by blocking cyclooxygenase 1 and 2.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
In HLEC-B3, bromfenac (0-80 μg/mL; 24 h) can block the epithelial-mesenchymal transition triggered by transforming growth factor-β2 in a concentration-dependent way[2]. In human anterior capsules, the transforming growth factor-β2-induced epithelial-mesenchymal transition is inhibited by bromfenac (80 μg/Ml; 48 h)[2].
ln Vivo
In rats, bromfenac (0.0032-3.16%; 100 or 200 μL; applied topically) has notable anti-inflammatory effects at doses as low as 0.1% (4 hours before treatment) or 0.32% (18 hours before treatment)[3]. When applied topically to the paws, bromfenac (0.032-3.16%; 100 μL) has dose-dependent anti-inflammatory effects in rats[3]. Applying bromfenac (0.032-1.0%; 50 μL) directly to the skin area of guinea pigs exposed to UV light is 26 times more effective than indomethacin at preventing erythema[3]. The amount of paw volume in both hind limbs of rats is reduced in a dose and time dependent manner when bromfenac (0.0032-0.1%; 50 μL) is rubbed onto the uninjected paw for four hours a day, five days a week[3]. When applied topically to the abdomen, bromfenac (0.32%; 50 μL) significantly inhibits mice's abdominal constriction in response to an ACh challenge[3]. When applied as eyedrops, 1 μL (0.09%) of bromfenac is applied twice daily for four weeks, partially reducing corneal staining, which becomes less noticeable by the fourth week[4].
Cell Assay
Cell Viability Assay[2]
Cell Types: transforming growth factor-β2-treated human anterior capsules.
Tested Concentrations: 80 μg/mL
Incubation Duration: 48 hrs (hours)
Experimental Results: Suppressed transforming growth factor-β2-induced epithelial-mesenchymal transition in primary lens epithelial cells (LECs).

Cell Migration Assay [2]
Cell Types: HLEC-B3 cells
Tested Concentrations: 0, 20, 40, 60, and 80 μg/ mL
Incubation Duration: 24 hrs (hours)
Experimental Results: Suppressed transforming growth factor-β2-induced cell migration in HLEC-B3 cells, and demonstrated inhibition of the over-expression of epithelial-mesenchymal transition markers.
Animal Protocol
Animal/Disease Models: Male SD (Sprague-Dawley) rats (150-250 g) are injected carrageenan[2]
Doses: 0.0032, 0.01, 0.032, 0.1, 0.32, 1.0, 3.16% (100 or 200 μL)
Route of Administration: Rubbed onto the backs before 1-72 h of injected carrageenan
Experimental Results: Produced significant anti-inflammatory activity when applied 1, 2, and 4 h prior to carrageenan challenge at 0.32%. Applied 1 or 4 h prior to carrageenan challenge was active, but not when applied 24 h (or longer) prior to carrageenan challenge at 0.2%.
References
[1]. Tetsuo Kida, et al. Pharmacokinetics and efficacy of topically applied nonsteroidal anti-inflammatory drugs in retinochoroidal tissues in rabbits. PLoS One. 2014 May 5;9(5):e96481.
[2]. Xiaobo Zhang, et al. Drug-eluting intraocular lens with sustained bromfenac release for conquering posterior capsular opacification. Bioact Mater. 2021 Jul 23;9:343-357.
[3]. Nolan JC, et, al. The topical anti-inflammatory and analgesic properties of bromfenac in rodents. Agents Actions. 1988 Aug; 25(1-2): 77-85.
[4]. Kaevalin Lekhanont, et al. Effects of topical anti-inflammatory agents in a botulinum toxin B-induced mouse model of keratoconjunctivitis sicca. J Ocul Pharmacol Ther. 2007 Feb;23(1):27-34.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C15H11BRNO3.NA
Molecular Weight
356.15
CAS #
91714-93-1
SMILES
BrC1C([H])=C([H])C(=C([H])C=1[H])C(C1=C([H])C([H])=C([H])C(C([H])([H])C(=O)[O-])=C1N([H])[H])=O.[Na+]
InChi Key
HZFGMQJYAFHESD-UHFFFAOYSA-M
InChi Code
InChI=1S/C15H12BrNO3.Na/c16-11-6-4-9(5-7-11)15(20)12-3-1-2-10(14(12)17)8-13(18)19;/h1-7H,8,17H2,(H,18,19);/q;+1/p-1
Chemical Name
2-Amino-3-(4-bromobenzoyl)benzeneacetic acid sodium
Synonyms
AHR 10282R; Bromfenac sodium, Prolensa, AHR-10282R;AHR10282R;Bromday, Xibrom, AHR-10282, AHR10282, AHR 10282
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO:71 mg/mL (199.4 mM)
Water:71 mg/mL (199.4 mM)
Ethanol: 2 mg/mL (5.6 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.8078 mL 14.0390 mL 28.0781 mL
5 mM 0.5616 mL 2.8078 mL 5.6156 mL
10 mM 0.2808 mL 1.4039 mL 2.8078 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

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