Brassinolide

Alias: Brassinolide
Cat No.:V3417 Purity: ≥98.48%
Brassinolide, a plant growth modulator and a plant hormone, is a sterol compound first isolated from pollen of rape (Brassica napus L.
Brassinolide Chemical Structure CAS No.: 72962-43-7
Product category: Apoptosis
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
25mg
50mg
100mg
250mg
500mg
Other Sizes

Other Forms of Brassinolide:

  • Epibrassinolide (24-Epibrassinolide; B1105; BP55)
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98.48%

Purity: ≥98%

Product Description

Brassinolide, a plant growth modulator and a plant hormone, is a sterol compound first isolated from pollen of rape (Brassica napus L.). It might facilitate cell division and stem cell elongation. Brassinolide may also have anticancer properties. It might cause PC-3 cells to exhibit a time- and concentration-dependent cytotoxicity. The predominant mode of cell death seemed to be apoptosis. Treatment with brassinolide may boost Caspase-3 activity. According to Western blot analyses, treatment with brassinolide led to a time-dependent reduction in the expression of the anti-apoptotic protein Bcl-2. Therefore, brassinolide might cause cytotoxicity in PC-3 cells by inducing apoptosis. Therefore, brassinolide may be a promising candidate for the treatment of prostate cancer.

Biological Activity I Assay Protocols (From Reference)
Targets
plant growth modulator
ln Vitro
A naturally occurring plant hormone called brassinolide encourages growth, boosts crop yields for grains and fruits, and makes plants more resilient to drought and cold temperatures. In PC-3 cells, brassinolide may cause a cytotoxicity that is dependent on both time and concentration. A time-dependent reduction in the expression of the anti-apoptotic protein Bcl-2 and a significant accumulation in the G2/M phase of the cell cycle are brought on by brassinolide treatment[1]. The level of protein expression of p53 in resistant cells is higher than that of sensitive cells. After Brassinolide treatment, the expression of p53 in CCRF-VCR cells restored to the level of sensitive cells. Brassinolide can effectively reverse the resistance of CCRF-VCR cells by inhibiting the effusion of drug transported by P-glucoprotein. To down regulate the abnormal expression of p53 maybe one of the mechanisms of reversing MDR for Brassinolide. MTT method is used to detect the resistant factor of resistant cell line and the reversing fold after addition of Brassinolide. The intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein is detected by flow cytometry, the catalytic activity of topoisomerase II is assessed by Sulliven method to find the effect of Brassinolide on resistance. The protein expression of p53 is measured using Western blotting in the sensitive cells and resistant cells to explore the effect of Brassinolide.

ln Vivo

Cell Assay
MTT method is used to detect the resistant factor of resistant cell line and the reversing fold after addition of Brassinolide. The effect of Brassinolide on resistance is determined by flow cytometry, which measures the intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein. To investigate the impact of brassinolide, Western blotting is used to quantify the protein expression of p53 in both resistant and sensitive cells.
Animal Protocol


References

[1]. Pharmazie . 2007 May;62(5):392-5.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C28H48O6
Molecular Weight
480.68
Exact Mass
480.34508925
CAS #
72962-43-7
Appearance
Solid
SMILES
C[C@@H]([C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2COC(=O)[C@@H]4[C@@]3(C[C@H]([C@H](C4)O)O)C)C)[C@H]([C@@H]([C@@H](C)C(C)C)O)O
InChi Key
IXVMHGVQKLDRKH-KNBKMWSGSA-N
InChi Code
InChI=1S/C28H48O6/c1-14(2)15(3)24(31)25(32)16(4)18-7-8-19-17-13-34-26(33)21-11-22(29)23(30)12-28(21,6)20(17)9-10-27(18,19)5/h14-25,29-32H,7-13H2,1-6H3/t15-,16-,17-,18+,19-,20-,21+,22-,23+,24+,25+,27+,28+/m0/s1
Chemical Name
(1S,2R,4R,5S,7S,11S,12S,15R,16S)-15-[(2S,3R,4R,5S)-3,4-dihydroxy-5,6-dimethylheptan-2-yl]-4,5-dihydroxy-2,16-dimethyl-9-oxatetracyclo[9.7.0.02,7.012,16]octadecan-8-one
Synonyms
Brassinolide
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ≥ 5 mg/mL
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.0804 mL 10.4019 mL 20.8039 mL
5 mM 0.4161 mL 2.0804 mL 4.1608 mL
10 mM 0.2080 mL 1.0402 mL 2.0804 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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