Bleomycin Sulfate

Alias: NSC-125066; BLEO; BLM; NSC 125066; BLEO cell; BLEO-cell; NSC125066; BLEOcell; Bleolem; Bleomycin sulfate; Trade name: Blenoxane. Blanoxan
Cat No.:V1424 Purity: ≥98%
Bleomycin Sulfate (also known as NSC125066; NSC-125066; BLEO-cell; Bleolem; Blenoxane; Blanoxan), the sulfate salt of Bleomycin, is a glycopeptide antibiotic and an anticancer agent approved for treating a variety of cancers including Hodgkin's lymphoma, non-Hodgkin's lymphoma, ovarian cancer, testicular cancer, and cervical cancer.
Bleomycin Sulfate Chemical Structure CAS No.: 9041-93-4
Product category: DNA(RNA) Synthesis
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
25mg
50mg
100mg
250mg
500mg
Other Sizes
Official Supplier of:
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text

 

  • Business Relationship with 5000+ Clients Globally
  • Major Universities, Research Institutions, Biotech & Pharma
  • Citations by Top Journals: Nature, Cell, Science, etc.
Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Bleomycin Sulfate (also known as NSC125066; NSC-125066; BLEO-cell; Bleolem; Blenoxane; Blanoxan), the sulfate salt of Bleomycin, is a glycopeptide antibiotic and an anticancer agent approved for treating a variety of cancers including Hodgkin's lymphoma, non-Hodgkin's lymphoma, ovarian cancer, testicular cancer, and cervical cancer. It exhibits strong anti-proliferative activity in vitro against a range of cancer cell lines, including squamous cell carcinomas, in UT-SCC-19A cells, where the IC50 is 4 nM. Combining the sulfate salts of basic glycopeptide antineoplastic antibiotics that were extracted from Streptomyces verticillus is bleomycin sulfate. It combines with iron to form complexes that convert molecular oxygen to superoxide and hydroxyl radicals, which damage DNA strands one way or both, as well as causing lipid peroxidation and the oxidation of carbohydrates, among other things.

Biological Activity I Assay Protocols (From Reference)
Targets
DNA/RNA Synthesis
ln Vitro

UT-SCC-12A and UT-SCC-12B possess an IC50 of 13. nM and 14.2 nM, respectively, making them both more resistant to bleomycin sulfate. [1] Incubated alveolar macrophages with 0.01 μg/mLto 1 μg/mL When macrophages are incubated with bleomycin sulfate for 18 hours, they secrete a significantly higher amount of fibroblast growth factor than when they are not. Even after the Bleomycin sulfate is removed and replaced with fresh (Bleomycin sulfate-free) media, macrophages stimulated with Bleomycin sulfate continue to produce a significant amount of fibroblast growth factor. Cycloheximide, NDGA (nordihydroguaiaretic acid), and BW755c, which are 5-lipoxygenase inhibitors, all inhibit the secretion of fibroblast growth factor by Bleomycin sulfate-stimulated alveolar macrophages. This suggests that full expression of activity requires not only protein synthesis but also metabolites of the 5-lipoxygenase pathway of arachidonic acid metabolism.[2] After a 24-hour incubation period with 400 µg/mL of bleomycin sulfate, NTera-2 cell viability is reduced. Additionally, caspase-3, -8, and -9 activities, Bax, and cytoplasmic cytochrome c levels are increased, while Bcl-2 levels are decreased.[3] Bleomycin sulfate has a clastogenic effect on ADIPO-P2 cells that lasts for at least 10 days in terms of unstable aberrations. The telomere instability caused by bleomycin sulfate in mammalian cells lasts for multiple generations following exposure. Furthermore, the observation of telomere fusions in cells exposed to bleomycin sulfate 10 days post-treatment implies that bleomycin sulfate can cause delayed telomere instability.[4]

ln Vivo
Day 7 post-bleomycin sulfate, CD45+ cells in BALf in NOX-/-is 1.7-fold > WT, with 57% of these being Mf, which by Day 21 decreases by 67% in WT and 83% in NOX-/-.[5]
Cell Assay
ADIPO-P2 cells are cultured in D-MEM high glucose medium at 37 °C with 5% CO2 atmosphere, supplemented with 20% fetal calf serum, penicillin (100 U/mL), and streptomycin (100 μg/mL). 1.5 × 105 cells/mL are cultured as monolayers in TC25 Corning flasks. Two flasks are set up for each experiment: one for the treated culture and one for the control. ADIPO-P2 cells are exposed to a 30-minute pulse of 2.5 μg/mL bleomycin sulfate during the log phase of growth. Parallel cultures serving as controls are not subjected to bleomycin sulfate. The duration and concentration of bleomycin sulfate exposure are selected based on earlier research using bleomycin sulfate exposure in mammalian cells conducted in our lab. The cells are maintained in culture with fresh culture medium until harvesting after being twice washed with Hank's balanced salt solution following the completion of the Bleomycin sulfate pulse treatment. After treatment, cells are kept in culture continuously for five passages or subcultures. When the cultures reach confluency (approximately 4 × 105 cells/mL of culture medium), subcultivation is performed. At the time of subcultivation, cells are collected by trypsinization, and the number of viable cells is determined by staining an aliquot of approximately 200 μL with 0.4% trypan blue. This process allows for the estimation of cell growth. Subsequently, the cells are suspended in new culture medium and added to fresh culture flasks with a density of 1 × 1055 cells/mL to continue growing. After the treatments are over, the remaining cells are either thrown away or transferred to another flask for cytogenetic analysis, which takes place 18 hours and 10 days later. Colchicine (0.1 μg/mL) is added to cell cultures in the final three hours of culture to analyze chromosomal aberrations. Standard protocols are followed when preparing chromosomes. Following harvesting, cells undergo hypotonic shock, are fixed in a 3:1 methanol:acetic acid solution, are spread out onto glass slides, and then undergo PNA-FISH processing. There are two separate experiments conducted.
Animal Protocol
Male Fischer 344 rats, 8-10 week old, weighing 150-250 g
3.5-4 mg/kg
Intra-tracheal
References

[1]. Acta Otolaryngol Suppl . 1997:529:241-4.

[2]. Am J Pathol . 1989 Feb;134(2):355-63.

[3]. Mol Med Rep . 2012 Jun;5(6):1481-6.

[4]. Mutat Res . 2012 Jun 1;734(1-2):5-11.

[5]. Stem Cell Res Ther . 2012 May 29;3(3):21.

[6]. Mutat Res . 2012 Sep 18;747(2):228-33.

[7]. Int J Clin Exp Med . 2014 Sep 15;7(9):2645-50. eCollection 2014.

[8]. Mol Cytogenet . 2016 Jun 21:9:49.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C55H85N17O25S4
Molecular Weight
1512.62
Exact Mass
1511.48
Elemental Analysis
C, 43.67; H, 5.66; N, 15.74; O, 26.44; S, 8.48
CAS #
9041-93-4
Appearance
Solid powder
SMILES
CC1=C(N=C(N=C1N)C(CC(=O)N)NCC(C(=O)N)N)C(=O)NC(C(C2=CN=CN2)O[C@H]3[C@H]([C@H]([C@@H]([C@@H](O3)CO)O)O)O[C@@H]4[C@H]([C@H]([C@@H]([C@H](O4)CO)O)OC(=O)N)O)C(=O)NC(C)C(C(C)C(=O)NC(C(C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.OS(=O)(=O)[O-]
InChi Key
WUIABRMSWOKTOF-OCBSMOPSSA-N
InChi Code
InChI=1S/C55H83N17O21S3.H2O4S/c1-20-33(69-46(72-44(20)58)25(12-31(57)76)64-13-24(56)45(59)82)50(86)71-35(41(26-14-61-19-65-26)91-54-43(39(80)37(78)29(15-73)90-54)92-53-40(81)42(93-55(60)88)38(79)30(16-74)89-53)51(87)66-22(3)36(77)21(2)47(83)70-34(23(4)75)49(85)63-10-8-32-67-28(18-94-32)52-68-27(17-95-52)48(84)62-9-7-11-96(5)6;1-5(2,3)4/h14,17-19,21-25,29-30,34-43,53-54,64,73-75,77-81H,7-13,15-16,56H2,1-6H3,(H13-,57,58,59,60,61,62,63,65,66,69,70,71,72,76,82,83,84,85,86,87,88);(H2,1,2,3,4)/t21?,22?,23?,24?,25?,29-,30+,34?,35?,36?,37+,38+,39-,40-,41?,42-,43-,53+,54-;/m0./s1
Chemical Name
3-[[2-[2-[2-[[2-[[4-[[2-[[6-amino-2-[3-amino-1-[(2,3-diamino-3-oxopropyl)amino]-3-oxopropyl]-5-methylpyrimidine-4-carbonyl]amino]-3-[(2R,3S,4S,5S,6S)-3-[(2R,3S,4S,5R,6R)-4-carbamoyloxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3-(1H-imidazol-5-yl)propanoyl]amino]-3-hydroxy-2-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]ethyl]-1,3-thiazol-4-yl]-1,3-thiazole-4-carbonyl]amino]propyl-dimethylsulfanium;hydrogen sulfate
Synonyms
NSC-125066; BLEO; BLM; NSC 125066; BLEO cell; BLEO-cell; NSC125066; BLEOcell; Bleolem; Bleomycin sulfate; Trade name: Blenoxane. Blanoxan
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~100 mg/mL (~66.1 mM)
Water: ~100 mg/mL (~66.1 mM)
Ethanol: <1 mg/mL
Solubility (In Vivo)
Saline: 30 mg/mL
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 0.6611 mL 3.3055 mL 6.6110 mL
5 mM 0.1322 mL 0.6611 mL 1.3222 mL
10 mM 0.0661 mL 0.3306 mL 0.6611 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
/

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
+
+
+

Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Clinical Trial Information
NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01858922 Active
Recruiting
Drug: ABVE-PC
Drug: DECA
Hodgkin Disease Baylor College of Medicine December 19, 2012 Phase 2
NCT03159897 Active
Recruiting
Drug: Doxorubicin
Drug: Bleomycin
Hodgkin Lymphoma Fondazione Italiana Linfomi - ETS August 1, 2017 Phase 3
NCT01712490 Active
Recruiting
Drug: bleomycin
Drug: vinblastine
Hodgkin Lymphoma Takeda November 9, 2012 Phase 3
NCT01873326 Active
Recruiting
Drug: Paclitaxel
Drug: Bleomycin
Germ Cell Tumors Memorial Sloan Kettering Cancer
Center
June 2013 Phase 2
NCT01347294 Active
Recruiting
Drug: Bleomycin + Fibrovein
Drug: Bleomycin
Venous Malformation Oslo University Hospital August 2011 Phase 4
Biological Data
  • Chromosomal content of micronuclei (MN) in binucleated human leukocytes. The MN in mitomycin C- and bleomycin-treated human leukocytes were sequentially analyzed by three-color FISH using centromeric probes (cep) and whole chromosome paints (wcp). Mol Cytogenet . 2016 Jun 21:9:49.
  • Int J Clin Exp Med . 2014 Sep 15;7(9):2645-50. eCollection 2014.
  • Increase in collagen content of lung post-bleomycin in gp91phox-/- but not in MM12-gp91phox double knockout mice. Stem Cell Res Ther . 2012 May 29;3(3):21.
  • Study design of BrdU pulse chase of control and bleomycin-induced lung fibrosis model in mice. Stem Cell Res Ther . 2012 May 29;3(3):21.
  • Cellularity in bronchoalveolar lavage fluid and lung before and after bleomycin challenge at day 7 in wildtype vs. gp91phox-/- and MMP12-gp91phox double knockout mice. Stem Cell Res Ther . 2012 May 29;3(3):21.
Contact Us