Bezafibrate

Alias: BM 15075; BM-15075; Bezafibrate;BM15075;Benzofibrate; Bezalip; Bezatrol; Difaterol; Cedur; Bezafibratum
Cat No.:V0836 Purity: ≥98%
Bezafibrate (BM 15075; BM-15075; Benzofibrate;BM15075; Bezalip; Bezatrol; Difaterol; Cedur; Bezafibratum) is an anti-hypertriglyceridemic/lipid-lowering drug acting.
Bezafibrate Chemical Structure CAS No.: 41859-67-0
Product category: PPAR
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
100mg
500mg
1g
Other Sizes

Other Forms of Bezafibrate:

  • Bezafibrate-d6
  • Bezafibrate-d4
Official Supplier of:
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Top Publications Citing lnvivochem Products
Product Description

Bezafibrate (BM 15075; BM-15075; Benzofibrate; BM15075; Bezalip; Bezatrol; Difaterol; Cedur; Bezafibratum) is an anti-hypertriglyceridemic/lipid-lowering drug acting. It acts as a potent agonist of PPAR (peroxisome proliferator-activated receptor alpha) with EC50s of 50 μM, 60 μM, 20 μM for human PPARα, PPARγ and PPARδ.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Bezafibrate is a PPAR agonist. For mouse PPARα, PPARγ, and PPARδ, the corresponding EC50 values are 90 μM, 55 μM, and 110 μM. Human PPARα, PPARγ, and PPARδ have respective EC50 values of 50 μM, 60 μM, and 20 μM. is [1]. When applied to human retinal pigment epithelial ARPE-19 cells and human retinal microvascular endothelial cells (HRMEC), bezafibrate (>200 μM) exhibits notable cytotoxicity. In HRMEC, bezafibrate (30-100 μM) reduces TNFα-induced inflammatory factors and controls TNFα-induced nuclear factor (NF)-κB transactivation. Bezafibrate inhibits the migration of HRMECs caused by VEGF and the release of VEGF by ARPE-19 cells stimulated by interleukin (IL)-1β [2].
ln Vivo
In TallyHo mice, bezafibrate (0.5%) markedly decreased plasma lipid and glucose levels and increased the size of the pancreatic islet. Bezafibrate also enhances metabolic flexibility and energy expenditure. Bezafibrate also enhances steatosis, modifies the makeup of lipids, and boosts the mass of mitochondria in the liver [3].
Animal Protocol
TallyHo mice are bred in our animal facility. Only male mice are used in the study, and mice receive a standard diet (SD), which is supplemented with 0.5% (w/w) Bezafibrate for the Bezafibrate groups for 8 weeks. Animals are killed by isoflurane overdose, and dissected tissues are prepared as stated below. All data represent samples taken after 8 weeks of Bezafibrate (or SD) treatment unless otherwise stated
Rats and mice
References
[1]. Willson TM, et al. The PPARs: from orphan receptors to drug discovery. J Med Chem. 2000 Feb 24;43(4):527-50.
[2]. Usui-Ouchi A, et al. The peroxisome proliferator-activated receptor pan-agonist bezafibrate suppresses microvascular inflammatory responses of retinal endothelial cells and vascular endothelial growth factor production in retinal pigmented epithelial cells. Int Immunopharmacol. 2017 Nov;52:70-76.
[3]. Franko A, et al. Bezafibrate ameliorates diabetes via reduced steatosis and improved hepatic insulin sensitivity in diabetic TallyHo mice. Mol Metab. 2017 Jan 6;6(3):256-266
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C19H20CLNO4
Molecular Weight
361.82
CAS #
41859-67-0
SMILES
ClC1C([H])=C([H])C(=C([H])C=1[H])C(N([H])C([H])([H])C([H])([H])C1C([H])=C([H])C(=C([H])C=1[H])OC(C(=O)O[H])(C([H])([H])[H])C([H])([H])[H])=O
InChi Key
IIBYAHWJQTYFKB-UHFFFAOYSA-N
InChi Code
InChI=1S/C19H20ClNO4/c1-19(2,18(23)24)25-16-9-3-13(4-10-16)11-12-21-17(22)14-5-7-15(20)8-6-14/h3-10H,11-12H2,1-2H3,(H,21,22)(H,23,24)
Chemical Name
2-[4-[2-[(4-chlorobenzoyl)amino]ethyl]phenoxy]-2-methyl-propanoic acid
Synonyms
BM 15075; BM-15075; Bezafibrate;BM15075;Benzofibrate; Bezalip; Bezatrol; Difaterol; Cedur; Bezafibratum
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 72 mg/mL (199.0 mM)
Water:<1 mg/mL
Ethanol: 18 mg/mL (49.7 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.7638 mL 13.8190 mL 27.6381 mL
5 mM 0.5528 mL 2.7638 mL 5.5276 mL
10 mM 0.2764 mL 1.3819 mL 2.7638 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
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