| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| ln Vitro |
According to ABTS and DPPH tests, bergaptol (0-800 ppm) exhibits definite activity [1]. In RAW264.7 cells, LPS-induced NO, IL-6, and TNF are inhibited by 50 μg/mL of Bergaptol for 24 hours. Additionally, LPS-induced MAPK phosphorylation and NF-κB activation are inhibited in RAW264.7 cells [3].
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| ln Vivo |
LPS-treated mice's cognitive function is improved when bergamot alcohol (10–40 mg/kg) is administered intraperitoneally once a day, during sleep [Disorder 4].
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| Cell Assay |
Western Blot Analysis[3]
Cell Types: Production of LPS--α[3]. Induction of RAW264.7 cells Tested Concentrations: 50 μg/mL Incubation Duration: 24 hrs (hours) Experimental Results: Inhibition of JNK phosphorylation and NF-κB activation. Inhibits the translocation of NF-κB P65 from the cytoplasm to the nucleus. |
| Animal Protocol |
Animal/Disease Models: LPS (40 μg/kg, icv) treated mice [4] ]
Doses: 10-40 mg/kg Route of Administration: intraperitoneal (ip) injection, one time/day for two weeks Experimental Results: diminished LPS-induced hippocampal CA1 area nerves Fixation and lysis of metanuclei (H&E staining). Increased dendritic spine density in mice. Inhibits LPS-induced neuroinflammation. Male C57BL/6 mice (6 weeks old) were randomly divided into 5 groups: Sham, LPS, LPS + Bergaptol (10 mg/kg), LPS + Bergaptol (20 mg/kg), and LPS + Bergaptol (40 mg/kg). Mice were anesthetized and fixed in a stereotaxic apparatus. LPS (40 μg/kg) or an equivalent volume of normal saline was injected into the hippocampal CA1 region (coordinates: 0.8 mm posterior to bregma, ±1.4 mm lateral, 4 mm depth) using a microsyringe. Bergaptol was dissolved in normal saline to 4 mg/mL and administered intraperitoneally once daily for two weeks. Sham and LPS groups received the same volume of normal saline [4]. After treatment, the Morris water maze test was performed to assess learning and memory. Mice were trained for 6 consecutive days to find a hidden platform, followed by a spatial probe test on day 7 where the platform was removed, and swimming tracks were recorded for 60 seconds [4]. Brain tissues were collected for histological analysis. For Golgi-Cox staining, brain tissue was immersed in impregnation solutions for two weeks, sectioned (100 μm), and stained. For H&E and Nissl staining, paraffin sections were prepared and stained with hematoxylin-eosin or 1% toluidine blue. For immunofluorescence, sections were incubated with primary antibodies against Iba-1, PSD-95, or synapsin-1, followed by fluorescent secondary antibodies, and observed by confocal microscopy [4]. Hippocampal tissue was homogenized for RNA extraction (RT-PCR) and protein extraction (ELISA) to measure TNF-α, IL-6, and IL-1β levels [4]. |
| References |
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| Additional Infomation |
Bergamot lactone belongs to the psoralen class of compounds and is a 5-hydroxyfuranocoumarin. It is the conjugated acid of bergamot lactone (1-). Bergamot lactone has been reported to exist in Angelica dahurica, plants of the genus Dorstenia, and other organisms with relevant data.
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| Molecular Formula |
C11H6O4
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|---|---|
| Molecular Weight |
202.165
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| Exact Mass |
202.026
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| CAS # |
486-60-2
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| PubChem CID |
5280371
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
311.9±11.0 °C at 760 mmHg
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| Melting Point |
287-290ºC
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| Flash Point |
142.4±19.3 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.711
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| LogP |
0.93
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
15
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| Complexity |
312
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
GIJHDGJRTUSBJR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H6O4/c12-10-2-1-6-9(15-10)5-8-7(11(6)13)3-4-14-8/h1-5,13H
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| Chemical Name |
4-hydroxyfuro[3,2-g]chromen-7-one
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| Synonyms |
Psoralin, 5-hydroxy- 5-Hydroxypsoralen
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 50 mg/mL (~247.33 mM)
H2O : ~0.67 mg/mL (~3.31 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (12.37 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9463 mL | 24.7317 mL | 49.4633 mL | |
| 5 mM | 0.9893 mL | 4.9463 mL | 9.8927 mL | |
| 10 mM | 0.4946 mL | 2.4732 mL | 4.9463 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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