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    Siponimod (BAF312)
    Siponimod (BAF312)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1505
    CAS #: 1230487-00-9Purity ≥98%

    Description: Siponimod (BAF-312; WHO-9491; NVP-BAF-312; Mayzent) is a novel, potent and orally bioavailable S1P receptor modulator with immunomodulating activity. It has high selectivity for S1P1 and S1P5 receptors (EC50 of 0.39 nM and 0.98 nM) and has the potential for treating medical conditions characterized by significant immunopathology. It exhibits >1000-fold selectivity over S1P2, S1P3 and S1P4 receptors. In vitro metabolism studies with liver microsomes have shown that the metabolic clearance of BAF312 is high in rat, low to moderate in monkey and human being, and low in dog and mouse. 

    References: Br J Pharmacol. 2012 Nov;167(5):1035-47; J Immunol. 2013 Apr 1;190(7):3533-40. 

    Related CAS #: 1234627-85-0 (fumarate)   1230487-00-9 (free base) 

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    Molecular Weight (MW)516.6
    FormulaC29H35F3N2O3
    CAS No.1230487-00-9
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 100 mg/mL (193.6 mM)
    Water: <1 mg/mL
    Ethanol: 44 mg/mL (85.2 mM)
    Other info

    Chemical Name: (E)-1-(4-(1-(((4-cyclohexyl-3-(trifluoromethyl)benzyl)oxy)imino)ethyl)-2-ethylbenzyl)azetidine-3-carboxylic acid

    InChi Key: KIHYPELVXPAIDH-HNSNBQBZSA-N

    InChi Code: InChI=1S/C29H35F3N2O3/c1-3-21-14-23(10-11-24(21)15-34-16-25(17-34)28(35)36)19(2)33-37-18-20-9-12-26(22-7-5-4-6-8-22)27(13-20)29(30,31)32/h9-14,22,25H,3-8,15-18H2,1-2H3,(H,35,36)/b33-19+

    SMILES Code: CCC1=C(CN2CC(C(O)=O)C2)C=CC(/C(C)=N/OCC3=CC(C(F)(F)F)=C(C4CCCCC4)C=C3)=C1

    Synonyms

    BAF-312; WHO 9491; NVP-BAF-312; BAF 312; WHO-9491; NVP-BAF312; BAF312; NVP-BAF312-NX; WHO9491; NVP-BAF 312; NVP-BAF312-AEA; Siponimod; Mayzent.


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    In Vitro

    In vitro activity: BAF312 (Siponimod) is a potent and selective S1P receptor agonist, with EC50 of 0.39 nM and 0.98 nM for S1P1 and S1P5receptors, exhibits >1000-fold selectivity over S1P2, S1P3 and S1P4 receptors. BAF312 (1 h at 1 μM) promotes prominent internalization of S1P1 receptors by 91%.


    Kinase Assay: The cells are homogenized and centrifuged at 26900 × g for 30 min at 4°C. Membranes are re-suspended in 20 mM HEPES (pH 7.4), 100 mM NaCl, 10 mM MgCl2, 1 mM EDTA and 0.1% fat-free BSA at 2–3 mg protein/mL. GTPγ[35S] binding assay is performed with the membranes (75 mg protein /mL in 50 mM HEPES, 100 mM NaCl, 10 mM MgCl2, 20 μg/mL saponin and 0.1% fat-free BSA (pH 7.4), 5 mg/mL with wheat-germ agglutinin-coated scintillation proximity assay-bead, and 10 μM GDP for 10–15 min. The GTPγ[35S]-binding reaction is started by the addition of 200 pM GTPγ[35S]. After 120 min at room temperature, the plates are centrifuged for 10 min at 300 × g and counted.


    Cell Assay: Agonist-mediated internalization of S1P1 receptors in CHO cells analysed by flow cytometry Myc-tag hS1P1 cells are incubated for 1 h with agonist at 37°C in standard culture medium followed by a PBS wash. An aliquot is kept on ice for 3 h, while another aliquot is left for 3 (or 12) h in culture medium (no agonist) at 37°C. The cells are then incubated either with 4 μg/mL monoclonal mouse anti C-myc IgG1 antibody or with isotype control mouse IgG1 for 60 min at 4°C, followed by an incubation with 1 μg/mL of Alexa488-labelled goat anti-mouse secondary conjugates. The cells are subjected to flow cytometry measurements using 10000 viable cells per sample.

    In VivoBAF312 effectively suppresses encephalomyelitis (EAE) in rats by internalizing S1P1 receptors, rendering them insensitive to the egress signal from lymph nodes. BAF312 significantly reduces clinical scores when dosed prophylactically or therapeutically in mice at 0.3 mg/kg.
    Animal modelEncephalomyelitis (EAE) model rat 
    Formulation & DosageSuspended in 1% aqueous carboxy-methylcellulose; 0.03, 0.3 and 3 mg/kg; oral givage 
    References

    Br J Pharmacol. 2012 Nov;167(5):1035-47; J Immunol. 2013 Apr 1;190(7):3533-40. 


    These protocols are for reference only. InvivoChem does not independently validate these methods.

     

    BAF312 (Siponimod)

    BAF312 suppresses ongoing disease symptoms in rat EAE. Br J Pharmacol. 2012 Nov;167(5):1035-47.
     

    BAF312 (Siponimod)

    BAF312 activates human atrial myocytes via S1P1. Effects of BAF312 and for comparison S1P on GIRK current recorded from acutely isolated human atrial myocytes. Br J Pharmacol. 2012 Nov;167(5):1035-47.
     

    BAF312 (Siponimod)

    Mean changes in ALC after multiple daily doses of BAF312 in healthy subjects. Br J Pharmacol. 2012 Nov;167(5):1035-47.


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