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Purity: ≥98%
B02 (also called RAD51-IN-02)is a potent and specific inhibitor of human RAD51 with an IC50 of 27.4 μM. In many types of cancer cells, RAD51 is overexpressed. Chemo- or radio-sensitive cancer cells are enhanced when RAD51 is downregulated by siRNA. B02 induces a heightened sensitivity to various agents that damage DNA and inhibits HR repair in human embryonic kidney (HEK) and breast cancer cells. B02 also makes decitabine-induced apoptosis and DNA damage in MM cells more pronounced”. B02 exhibits a high degree of specificity for RAD51 and does not substantially inhibit RAD54 across a concentration range of 0 to 200 μM.
| Targets |
hRAD51 ( IC50 = 27.4 μM )
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| Cell Assay |
The WST-1 colorimetric cell count assay is used to track the proliferation of MM-cell lines. Approximately 8000 cells/well are seeded in 96-well plates with MM cell lines. After one hour of treatment with or without B02 (10 μM), the cells are treated for a further 72 hours with either vehicle (DMSO) or DOX (20–160 nM). Each well's culture medium receives a 1:10 addition of WST-1 reagent, and the incubation process lasts for four hours at 37°C. With a spectrophotometer, the absorbance at 450 nm is used to estimate the relative cell number.
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| Animal Protocol |
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| References |
| Molecular Formula |
C22H17N3O
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| Molecular Weight |
339.39
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| Exact Mass |
339.14
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| Elemental Analysis |
C, 77.86; H, 5.05; N, 12.38; O, 4.71
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| CAS # |
1290541-46-6
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| Appearance |
Solid powder
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| SMILES |
C1=CC=C(C=C1)CN2C(=NC3=CC=CC=C3C2=O)/C=C/C4=CN=CC=C4
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| InChi Key |
GEKDQXSPTHHANP-OUKQBFOZSA-N
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| InChi Code |
InChI=1S/C22H17N3O/c26-22-19-10-4-5-11-20(19)24-21(13-12-17-9-6-14-23-15-17)25(22)16-18-7-2-1-3-8-18/h1-15H,16H2/b13-12+
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| Chemical Name |
3-benzyl-2-[(E)-2-pyridin-3-ylethenyl]quinazolin-4-one
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (29.46 mM) in 20% DMSO 20% Cremophor EL + 60% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2 mg/mL (5.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2 mg/mL (5.89 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9465 mL | 14.7323 mL | 29.4646 mL | |
| 5 mM | 0.5893 mL | 2.9465 mL | 5.8929 mL | |
| 10 mM | 0.2946 mL | 1.4732 mL | 2.9465 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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